A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{lacW}l(2)35Dfk14423 Insertion

General Information
Symbol Dmel\P{lacW}l(2)35Dfk14423 Species D. melanogaster
Name FlyBase ID FBti0004835
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data
Affected gene(s) Ecol\lacZ, l(2)35Df Viability / fertility
Causes allele(s) Ecol\lacZl(2)35Df-k14423, l(2)35Dfk14423 Stock availability 2 publicly available
LINE ID l(2)k14423
Genomic Location
Chromosomal location 2L ( 35D4 ) Sequence location 2L:15,752,033..15,752,476 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0004218 FBti0132624 FBti0057583 FBti0043943 FBti0043493 FBti0106823 FBti0047589 FBti0056371 FBti0005357 FBti0011240 FBti0107784 FBti0068645 FBti0054553 FBti0013258 FBti0110451 FBti0112384 FBti0026216 FBti0013257 FBti0005358 FBti0007244 FBti0124810_2 FBti0124810_1 FBti0105306 FBti0024958 FBti0105378 FBti0107506 FBti0036402 FBti0048689 FBti0035289 FBti0018550 FBti0111861 FBti0115656 FBti0047170 FBti0004835 FBti0025982 FBti0114821 FBti0065821 FBti0057653 FBti0143741 FBti0143724
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Spradling et al., 1999, Bellen et al., 2004)
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Description
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FB2013_03
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:15,752,033..15,752,476 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
35D4 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
35D5-35D7 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ073304
(BDGP Project Members, 1994-1999)
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999)
l(2)35Df
(Ashburner et al., 1999, Roote, 1995.5.4)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZl(2)35Df-k14423
(BDGP Project Members, 1994-1999)
l(2)35Dfk14423
(Roote, 1995.5.4, BDGP Project Members, 1994-1999, Spradling et al., 1999, Ashburner et al., 1999.10.12, Ashburner et al., 1999, Misra and Rehm, 1999.3.12, Ashburner et al., 1999)
Lethality
References
lethal
(Ashburner et al., 1999)
lethal | recessive
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Ashburner et al., 1999)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k14423
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
12177
y1 w67c23; P{lacW}l(2)35Dfk14423/CyO
Kyoto
111433
yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}l(2)35Dfk14423 P{neoFRT}40A/CyO y+
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FlyBase curator comment: The sequence accession record G01316 includes the description \'fruit fly STS Dm2137 clone l(2)k14423\' however the sequence stated in G01316 BLASTs to the heix gene, not l(2)35Df which has been shown genetically to correspondThe actual flanking sequence is:GCGCTGCTTTTTGCCTGATAGAAGCTGCCAATATATTATTACTATATATATATATACATGTTATTAGTCTTCGAATTAAATTCGAATATACCAATCATATTTCCATAATAAAGTACAACATAATTTCTAACTATTATATTTTTTTTTCTTTTTTTGAACTAATTCAAAAATAGCTTAATTTCCCCTCAAAATAGTCAGATGGTCTGCACTGACGATGGTCGGCAGTATCGATAACCATGTCTATCGCGTTATCGGGCCTTCACATGTTTTGCGGCGTGCGTTGAAATAGTTCGTAAAAA
(Misra and Rehm, 1999.3.12)
Accession G01316 appears to be erroneously identified as l(2)k14423.
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}l(2)35Dfk14423
(Misra and Rehm, 1999.3.12, Roote, 1995.5.4, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 12 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Ashburner et al., 1999, Genetics 153(1): 179--219
An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster. The Adh region. [FBrf0111326]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material
Ashburner et al., 1999, Genetics 153(1):
Table S1. P-element insertions in the Adh region. [FBrf0112150]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
Ashburner et al., 1999.10.12, Adh.
Adh. [FBrf0112153]
Misra and Rehm, 1999.3.12, k14423 insertion.
k14423 insertion. [FBrf0183223]
Roote, 1995.5.4, Genetics 126: 679 help please.
Genetics 126: 679 help please. [FBrf0079819]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]