Dmel\P{lacW}SmD3^k13107 Insertion

General Information
Symbol	Dmel\P{lacW}SmD3^k13107	Species	D. melanogaster
Name		FlyBase ID	FBti0004847
Feature type	transposable_element_insertion_site	Created / Updated	1998-01-03/2003-04-03
Description
Inserted element	P{lacW}	Localized function
Affected gene(s)	Oda, SmD3	Expression data
Causes allele(s)	Oda^k13107, SmD3^k13107	Viability / fertility
LINE ID	l(2)k13107	Stock availability
Genomic Location
Chromosomal location	2R ( 48E4 )	Sequence location
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	48E4 ( near gene of known cytology )
Cytological location (reported)	48E4-48E7 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Chromosome in situ
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lacW}
Location-dependent role
Size	10.691Kb
Associated alleles	w^+mC Ecol\lacZ^P\T.W
Molecular map
Transposon class
Element type
Affected Gene(s)
Insertion within gene
Insertion may affect gene	Oda (Torok et al., 1993) SmD3 (Schenkel et al., 1999, BDGP Project Members, 1994-1999, Torok et al., 1993, Schenkel et al., 2002, Roch et al., 1998)
Orientation (relative to gene)	Gene Orientation References
Alleles and Phenotypes
Causes alleles	Oda^k13107 (Torok et al., 1993, BDGP Project Members, 1994-1999, Schenkel et al., 2002) SmD3^k13107 (Schenkel et al., 1999, BDGP Project Members, 1994-1999, Torok et al., 1993, Schenkel et al., 2002, Roch et al., 1998)

Lethality References lethal \| larval stage (Schenkel et al., 2002) lethal \| larval stage \| recessive (Schenkel et al., 2002) lethal \| recessive (Roch et al., 1998) lethal \| pupal stage (Torok et al., 1993)
Sterility References
Phenotype Manifest In
	imaginal disc (Roch et al., 1998) lymph gland (Torok et al., 1993) dorsal mesothoracic disc (Torok et al., 1993) larval brain (Torok et al., 1993)
Detailed Description
Statement Reference Brain is of normal size but abnormal shape. Wing and haltere discs rarely showing hyperplastic outgrowths, folding abnormal. Lymph gland retains lobular structure. (Torok et al., 1993) Brain, imaginal disc and blood cell neoplastic phenotype. (Mechler, 1994) Lethality occurs during third instar larval or pupal stages. Mutants exhibit visible disc abnormalities. In tergites, clones with normal size and frequency are recovered. Clones cannot be recovered in the wing disc. (Roch et al., 1998)
Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k13107 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line	t (Gene Disruption Project members, 2001-)
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu). (FlyBase, 1992-) NOTE: derived from line carrying multiple insertions. (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	l(2)k13107 (FlyBase, 1992-) P{lacW}guf^k13107 (Torok et al., 1993, FlyBase, 1992-) P{lacW}l(2)k09037^k13107 (Torok et al., 1993, FlyBase, 1992-) P{lacW}SmD3^k13107 (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0003186
References ( 12 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Schenkel et al., 2002, Genetics 161(2): 763--772 P elements inserted in the vicinity of or within the Drosophila snRNP SmD3 gene nested in the first intron of the Ornithine decarboxylase antizyme gene affect only the expression of SmD3. [FBrf0149027] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Ivanov et al., 1998, Molec. Cell. Biol. 18(3): 1553--1561 The Drosophila gene for antizyme requires ribosomal frameshifting for expression and contains an intronic gene for snRNP Sm D3 on the opposite strand. [FBrf0100578] Roch et al., 1998, Molec. gen. Genet. 257(2): 103--112 Screening of larval/pupal P-element induced lethals on the second chromosome in Drosophila melanogaster: clonal analysis and morphology of imaginal discs. [FBrf0100624] Salzberg et al., 1996, Genetics 144(1): 183--196 gutfeeling, a Drosophila gene encoding an antizyme-like protein, is required for late differentiation of neurons and muscles. [FBrf0089808] Mechler, 1994, J. Biosci., Bangalore 19(5): 537--556 When the control is lacking-the role of tumour suppressor genes in cancer development. [FBrf0080244] Torok et al., 1993, Genetics 135(1): 71--80 P-lacW insertional mutagenesis on the second chromosome of Drosophila melanogaster: isolation of lethals with different overgrowth phenotypes. [FBrf0058618]
Abstract	Schenkel et al., 1999, Europ. Dros. Res. Conf. 16: 214 Down-regulation of the expression of the spliceosome core protein SmD3 gene results in tissue overgrowth. [FBrf0110736]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}SmD3k13107 Insertion

Dmel\P{lacW}SmD3^k13107 Insertion