A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Dmel\P{lacW}SmD3k15401 Insertion

General Information
Symbol Dmel\P{lacW}SmD3k15401 Species D. melanogaster
Name FlyBase ID FBti0004848
Feature type transposable_element_insertion_site Created / Updated 1998-01-03/2003-04-03
Description
Inserted element P{lacW} Localized function
Affected gene(s) Oda, SmD3 Expression data
Causes allele(s) Odak15401, SmD3k15401 Viability / fertility
LINE ID l(2)k15401 Stock availability
Genomic Location
Chromosomal location 2R ( 48E4 ) Sequence location
hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location
(computed by FlyBase)
48E4 ( near gene of known cytology )
Cytological location
(reported)
Chromosome in situ
hide Sequence Data
Flanking sequence
hide Inserted Element
Construct P{lacW}
Location-dependent
role 		 
Size 10.691Kb
Associated alleles
w+mC
Ecol\lacZP\T.W
Molecular map
Transposon class
Element type
hide Affected Gene(s)
Insertion within gene
Insertion may
affect gene
Oda
(Torok et al., 1993)
SmD3
(Schenkel et al., 1999, BDGP Project Members, 1994-1999, Schenkel et al., 2002, Torok et al., 1993)
Orientation
(relative to gene)
Gene
Orientation
References
 
 
 
hide Alleles and Phenotypes
Causes alleles
Odak15401
(Torok et al., 1993, BDGP Project Members, 1994-1999, Schenkel et al., 2002)
SmD3k15401
(Schenkel et al., 1999, BDGP Project Members, 1994-1999, Schenkel et al., 2002, Torok et al., 1993)
Lethality
References
lethal | larval stage
(Schenkel et al., 2002)
lethal
(Torok et al., 1993)
lethal | larval stage | recessive
(Schenkel et al., 2002)
Sterility
References
hide Phenotype Manifest In
lymph gland
(Torok et al., 1993)
larval brain
(Torok et al., 1993)
hide Detailed Description
Statement
Reference
Lethality occurs during larval and pupal stages. Brain is smaller than wild type with abnormal shape. Discs are smaller than wild type with abnormal folding. Both discs and brain can exceed normal size at the end of larval life. Lymph gland retains lobular structure.
(Torok et al., 1993)
Brain, imaginal disc and blood cell neoplastic phenotype.
(Mechler, 1994)
hide Expression Data
Reporter Expression
Stage
Tissue/Position
Reference
Additional Information
Statement
Reference
Assay mode
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k15401
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Kyoto
102962
Df(1)w67c23, y1 ; P{lacW}SmD3k15401 / CyO
hide Linkouts
hide Comments
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).
(FlyBase, 1992-)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
l(2)k15401
(FlyBase, 1992-)
P{lacW}gufk15401
(Torok et al., 1993, FlyBase, 1992-)
P{lacW}l(2)k09037k15401
(Torok et al., 1993, FlyBase, 1992-)
P{lacW}SmD3k15401
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0003187
hide References ( 11 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Schenkel et al., 2002, Genetics 161(2): 763--772
P elements inserted in the vicinity of or within the Drosophila snRNP SmD3 gene nested in the first intron of the Ornithine decarboxylase antizyme gene affect only the expression of SmD3. [FBrf0149027]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Ivanov et al., 1998, Molec. Cell. Biol. 18(3): 1553--1561
The Drosophila gene for antizyme requires ribosomal frameshifting for expression and contains an intronic gene for snRNP Sm D3 on the opposite strand. [FBrf0100578]
Salzberg et al., 1996, Genetics 144(1): 183--196
gutfeeling, a Drosophila gene encoding an antizyme-like protein, is required for late differentiation of neurons and muscles. [FBrf0089808]
Mechler, 1994, J. Biosci., Bangalore 19(5): 537--556
When the control is lacking-the role of tumour suppressor genes in cancer development. [FBrf0080244]
Torok et al., 1993, Genetics 135(1): 71--80
P-lacW insertional mutagenesis on the second chromosome of Drosophila melanogaster: isolation of lethals with different overgrowth phenotypes. [FBrf0058618]
Abstract
Schenkel et al., 1999, Europ. Dros. Res. Conf. 16: 214
Down-regulation of the expression of the spliceosome core protein SmD3 gene results in tissue overgrowth. [FBrf0110736]
FlyBase analysis
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]