A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{lacW}DNApol-α60j10B2 Insertion

General Information
Symbol Dmel\P{lacW}DNApol-α60j10B2 Species D. melanogaster
Name FlyBase ID FBti0004882
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data lacZ reporter
Affected gene(s) DNApol-α60, Ecol\lacZ Viability / fertility
Causes allele(s) DNApol-α60j10B2, Ecol\lacZDNApol-α60-j10B2 Stock availability 1 publicly available
LINE ID l(3)j10B2
Genomic Location
Chromosomal location 3L ( 77B6 ) Sequence location 3L:20,354,650..20,354,650 [+]
Map ( GBrowse ) detailed view FBti0125825_2 FBti0125825_1 FBti0042510 FBti0004882 FBti0065451 FBti0046980 FBti0041239 FBti0127654 FBti0057904
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Description
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:20,354,650..20,354,650 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
77B6 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026282
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
DNApol-α60
(BDGP Project Members, 1994-1999)
Ecol\lacZ
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
DNApol-α60j10B2
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Beaton, 2000.1.31, Chen et al., 2000)
Ecol\lacZDNApol-α60-j10B2
(BDGP Project Members, 1994-1999)
Lethality
References
lethal | recessive
(Spradling et al., 1999, BDGP Project Members, 1994-1999, Beaton, 2000.1.31)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
Expression of DNAprimScer\UAS.cCa under the control of Scer\GAL4Act5C.PI partially rescues DNAprimj10B2 homozygotes; flies are viable but nearly sterile. Expression of DNAprimScer\UAS.cCa under the control of Scer\GAL4Act5C.PI partially rescues DNAprimj10B2/DNAprimSfaf240 flies; a few adult escapers are seen that die after 1 day.
(Chen et al., 2000)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
In wandering third instar larva, no detectable β-gal staining.
(Meister and Braun, 1995.10)
Marker for
Reflects
expression of
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)j10B2
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
12108
y1 w*; P{lacW}DNApol-α60j10B2/TM3, Sb1
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hide Comments
Location 3L:20295391-20295392 confirmed by FlyBase alignment of dbGSS accession AQ026282 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P2108
(Meister and Braun, 1995.10)
P{lacW}DNApol-α60j10B2
 
P{lacW}DNAprimj10B2
(FlyBase, 1992-, FlyBase, 2005)
P{lacW}j10B2
(Chen et al., 2000)
P{lacW}l(3)j10B2j10B2
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{w+mC=lacW}l(3)j10B2j10B2
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0001156
hide References ( 10 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Chen et al., 2000, Genetics 156(4): 1787--1795
Genetic analysis of the Drosophila DNAprim gene: The function of the 60-kd primase subunit of DNA polymerase opposes the fat facets signaling pathway in the developing eye. [FBrf0131274]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase
Beaton, 2000.1.31, BDGP P element Update.
BDGP P element Update. [FBrf0126832]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]