A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{lacW}dsx-c73Aj10E8 Insertion

General Information
Symbol Dmel\P{lacW}dsx-c73Aj10E8 Species D. melanogaster
Name FlyBase ID FBti0004886
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data lacZ reporter
Affected gene(s) CG33158, dsx-c73A, Ecol\lacZ Viability / fertility
Causes allele(s) CG33158j10E8, dsx-c73Aj10E8, Ecol\lacZj10E8 Stock availability 2 publicly available
LINE ID l(3)j10E8
Genomic Location
Chromosomal location 3L ( 73A1 ) Sequence location 3L:16,444,836..16,444,836 [+]
Map ( GBrowse ) detailed view FBti0020123 FBti0143633 FBti0141077 FBti0052678 FBti0068019 FBti0004886 FBti0028581 FBti0029262 FBti0027931 FBti0029345 FBti0056342 FBti0011503 FBti0045924 FBti0058277 FBti0144096
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Description
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:16,444,836..16,444,836 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
73A1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
73A1-73A2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ034081
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
CG33158
(FlyBase Curators, 2008)
dsx-c73A
(BDGP Project Members, 1994-1999)
Ecol\lacZ
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
CG33158j10E8
(FlyBase Curators, 2008)
dsx-c73Aj10E8
(BDGP Project Members, 1994-1999, Spradling et al., 1999)
Ecol\lacZj10E8
(BDGP Project Members, 1994-1999)
Lethality
References
lethal
(Andrew and Baker, 2008)
lethal | recessive
(Spradling et al., 1999, BDGP Project Members, 1994-1999)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
Larval cuticle from dsx-c73A[j10E8] mutant larvae have a completely normal pattern of denticles and hairs, and the A4 denticles appear morphologically normal.
(Andrew and Baker, 2008)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
In wandering third instar larva, no detectable β-gal staining.
(Meister and Braun, 1995.10)
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)j10E8
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
12097
y1 w*; P{lacW}dsx-c73Aj10E8, CG33158j10E8/TM3, Sb1
Kyoto
111400
yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}dsx-c73Aj10E8 P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb1
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hide Comments
Location 3L:16416995-16416996 confirmed by FlyBase alignment of dbGSS accession AQ034081 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P2097
(Meister and Braun, 1995.10)
P{lacW}dsx-c73Aj10E8
 
P{lacW}l(3)j10E8j10E8
(FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{w+mC=lacW}l(3)j10E8j10E8
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0001157
hide References ( 10 )
Research paper
Andrew and Baker, 2008, Dev. Dyn. 237(4): 1198--1206
Expression of the Drosophila secreted cuticle protein 73 (dsc73) requires shavenbaby. [FBrf0204322]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis
FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations.
Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]