Dmel\P{lacW}Nmt^j1C7 Insertion

General Information
Symbol	Dmel\P{lacW}Nmt^j1C7	Species	D. melanogaster
Name		FlyBase ID	FBti0004908
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, Nmt	Viability / fertility
Causes allele(s)	Ecol\lacZ^Nmt-j1C7, Nmt^j1C7	Stock availability	2 publicly available
LINE ID	l(3)j1C7
Genomic Location
Chromosomal location	3L ( 66B11 )	Sequence location	3L:8,171,259..8,171,259 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{lacW}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800). Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:8,171,259..8,171,259 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	66B11 ( inferred by FlyBase from sequence location )
Cytological location (reported)	66B10-66B11 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ026293 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) Nmt (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^Nmt-j1C7 (BDGP Project Members, 1994-1999) Nmt^j1C7 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Beaton, 2000.1.31, Ntwasa et al., 2001, Nicholls and Gelbart, 1998)

Lethality References lethal \| embryonic stage \| recessive (Ntwasa et al., 2001) lethal \| recessive (BDGP Project Members, 1994-1999, Beaton, 2000.1.31, Spradling et al., 1999)
Sterility References
Phenotype Manifest In
	cephalopharyngeal skeleton (Ntwasa et al., 2001) denticle belt (Ntwasa et al., 2001) dorsal closure embryo (Ntwasa et al., 2001) embryonic epidermis (Ntwasa et al., 2001) embryonic leading edge cell (Ntwasa et al., 2001) extended germ band embryo (Ntwasa et al., 2001) filzkorper (Ntwasa et al., 2001) presumptive embryonic/larval central nervous system (Ntwasa et al., 2001) presumptive embryonic/larval digestive system (Ntwasa et al., 2001) ventral nerve cord (Ntwasa et al., 2001)
Detailed Description
Statement Reference Homozygous embryos show a spectrum of developmental defects. Some embryos form the normal number of segments but have severely compressed ventral denticle belts, indicating that head involution is defective. Some embryos lack some denticle belts and have misplaced filzkorper, indicating defects in head involution and germ-band retraction. The sclerotised mouthparts sometimes show chaotic positioning. The posterior spiracles are sometimes found in the middle of the embryo. Failure of dorsal closure and stalling of gut development are seen in mutant embryos. Some embryos show degeneration or general thinning of the central nervous system (CNS), while in others the CNS appears largely normal except for a constriction at parasegment 10. This constriction is progressive and eventually results in the complete scission of the ventral nerve chord. Cells associated with the constriction are seen to undergo apoptosis. Mutant embryos in which dorsal closure has failed have abnormal actin patterns, with severe disruptions of the leading edge cells. Mutant embryos show widespread ectopic apoptosis compared to wild-type embryos. (Ntwasa et al., 2001)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference wandering third instar larval stage gonad (Meister and Braun, 1995.10)
Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)j1C7 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	12071 y¹ w^*; P{lacW}Nmt^j1C7/TM3, Sb¹
Kyoto	111383 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}Nmt^j1C7 P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb¹
Linkouts
Comments
	Location 3L:8152106-8152107 confirmed by FlyBase alignment of dbGSS accession AQ026293 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P2071 (Meister and Braun, 1995.10) P{lacW}l(3)j1C7^j1C7 (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{lacW}Nmt^j1C7 (FlyBase, 2005, FlyBase, 1992-, ) P{w^+mC=lacW}l(3)j1C7^j1C7 (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0001163
References ( 14 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Ntwasa et al., 2001, Exp. Cell Res. 262(2): 134--144 Drosophila embryos lacking N-myristoyltransferase have multiple developmental defects. [FBrf0132312] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Nicholls and Gelbart, 1998, Genetics 149(1): 203--215 Identification of chromosomal regions involved in decapentaplegic function in Drosophila. [FBrf0102843] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material	Parks et al., 2004, Nature Genetics 36(3): Supplementary Table 1: Deletions generated at Bloomington between trans-heterozygous P element insertions. [FBrf0179070]
Personal communication to FlyBase	Deal and Cook, 2002.2.25, Isolation and characterization of Df(3L)BSC13. Isolation and characterization of Df(3L)BSC13. [FBrf0145643] Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] Beaton, 2000.1.31, BDGP P element Update. BDGP P element Update. [FBrf0126832] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lacW}Nmtj1C7 Insertion

Dmel\P{lacW}Nmt^j1C7 Insertion