A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{lacW}Uch-L3j2B8 Insertion

General Information
Symbol Dmel\P{lacW}Uch-L3j2B8 Species D. melanogaster
Name FlyBase ID FBti0004923
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, Uch-L3 Viability / fertility
Causes allele(s) Ecol\lacZUch-L3-j2B8, Uch-L3j2B8 Stock availability 1 publicly available
LINE ID l(3)j2B8
Genomic Location
Chromosomal location 3L ( 67B9 ) Sequence location 3L:9,470,134..9,470,134 [+]
Map ( GBrowse ) detailed view FBti0048108 FBti0070858 FBti0027398 FBti0114166 FBti0004923 FBti0024074 FBti0106715 FBti0106382 FBti0070985 FBti0100030 FBti0112371 FBti0040084 FBti0048111 FBti0076381
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Description
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This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:9,470,134..9,470,134 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
67B9 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
67B10-67B11 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026303
(FlyBase, 1992-)
AQ034128
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999)
Uch-L3
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZUch-L3-j2B8
(BDGP Project Members, 1994-1999)
Uch-L3j2B8
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Fernandez-Funez et al., 2000, Beaton, 2000.1.31)
Lethality
References
lethal | recessive
(Spradling et al., 1999, Ren et al., 2005, Beaton, 2000.1.31)
semi-lethal
 
semi-lethal | recessive
(Berkeley Drosophila Genome Project, 1993.6.1)
Sterility
References
hide Phenotype Manifest In
arista | pharate adult stage
(Ren et al., 2005)
eye
(Berkeley Drosophila Genome Project, 1993.6.1)
fourth segment of antenna | pharate adult stage
(Ren et al., 2005)
leg | pharate adult stage
(Ren et al., 2005)
tarsal segment & joint & pharate adult
(Ren et al., 2005)
wing | P-stage
(Ren et al., 2005)
wing hair | P-stage
(Ren et al., 2005)
hide Detailed Description
Statement
Reference
Homozygous pharate adults have defects such as loss of tarsal leg joints, shorter and fatter leg segments than normal, the loss of a discrete antennal segment 4 and a fatter arista than normal. Homozygous pupal wings are wider and shorter than normal and have regions with a loss of hair.
(Ren et al., 2005)
Homozygotes have eye defects.
(Berkeley Drosophila Genome Project, 1993.6.1)
visible eye defects
 
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage  
imaginal disc  
(Meister and Braun, 1995.10)
Additional Information
Statement
Reference
In wandering third instar larva, expression in imaginal discs (which discs not specified).
(Meister and Braun, 1995.10)
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)j2B8
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
12078
y1 w*; P{lacW}Uch-L3j2B8/TM3, Sb1
hide Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P2078
(Meister and Braun, 1995.10)
P{lacW}l(3)j2B8j2B8
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{lacW}Uch-L3j2B8
(FlyBase, 1992-)
P{w+mC=lacW}l(3)j2B8j2B8
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0001169
hide References ( 11 )
Research paper
Ren et al., 2005, Genetics 171(2): 625--638
Gene expression during Drosophila wing morphogenesis and differentiation. [FBrf0190767]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Fernandez-Funez et al., 2000, Nature 408(6808): 101--106
Identification of genes that modify ataxin-1-induced neurodegeneration. [FBrf0131287]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase
Beaton, 2000.1.31, BDGP P element Update.
BDGP P element Update. [FBrf0126832]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
Berkeley Drosophila Genome Project, 1993.6.1, Insertion alleles.
Insertion alleles. [FBrf0122635]
FlyBase analysis
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]