A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{lacW}l(3)j2E11j2E11 Insertion

General Information
Symbol Dmel\P{lacW}l(3)j2E11j2E11 Species D. melanogaster
Name FlyBase ID FBti0004932
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data lacZ reporter
Affected gene(s) btl, Ecol\lacZ, l(3)j2E11 Viability / fertility
Causes allele(s) Ecol\lacZj2E11, l(3)j2E11j2E11 Stock availability 2 publicly available
LINE ID l(3)j2E11
Genomic Location
Chromosomal location 3L ( 70D2 ) Sequence location
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Description
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location
(computed by FlyBase)
70D2 ( near gene of known cytology )
Cytological location
(reported)
70C5-70C6 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026309
(FlyBase, 1992-)
AQ034129
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
btl
(BDGP Project Members, 1994-1999)
Ecol\lacZ
(BDGP Project Members, 1994-1999, Netter et al., 1998)
l(3)j2E11
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Burmester et al., 2000, Netter et al., 1998)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZj2E11
(BDGP Project Members, 1994-1999, Netter et al., 1998)
l(3)j2E11j2E11
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Burmester et al., 2000, Netter et al., 1998)
Lethality
References
lethal | recessive
(BDGP Project Members, 1994-1999, Spradling et al., 1999)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
In wandering third instar larva, no detectable β-gal staining.
(Meister and Braun, 1995.10)
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)j2E11
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
12086
y1 w*; P{lacW}l(3)j2E11j2E11/TM3, Sb1
Kyoto
111391
yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}l(3)j2E11j2E11 P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb1
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hide Comments
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).
(FlyBase, 1992-)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P2086
(Meister and Braun, 1995.10)
P{lacW}devj2E11
(FlyBase, 1992-)
P{lacW}l(3)j2E11j2E11
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{w+mC=lacW}l(3)j2E11j2E11
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0001174
hide References ( 9 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Burmester et al., 2000, Gene 246(1-2): 157--167
Genetic and molecular analysis in the 70CD region of the third chromosome of Drosophila melanogaster. [FBrf0127030]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Netter et al., 1998, Genetics 149(1): 257--275
white+ transgene insertions presenting a dorsal/ventral pattern define a single cluster of homeobox genes that is silenced by the polycomb-group proteins in Drosophila melanogaster. [FBrf0102846]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]