Dmel\P{lacW}how^j5B5 Insertion

General Information
Symbol	Dmel\P{lacW}how^j5B5	Species	D. melanogaster
Name		FlyBase ID	FBti0004971
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, how	Viability / fertility
Causes allele(s)	Ecol\lacZ^how-j5B5, how^j5B5	Stock availability	2 publicly available
LINE ID	l(3)j5B5
Genomic Location
Chromosomal location	3R ( 94A1 )	Sequence location	3R:17,867,954..17,867,954 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	3R:17,867,954..17,867,954 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	94A1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	94A1-94A2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ034089 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) how (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^how-j5B5 (BDGP Project Members, 1994-1999) how^j5B5 (BDGP Project Members, 1994-1999, Spradling et al., 1999)

Lethality References lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference wandering third instar larval stage embryonic/larval trachea (Meister and Braun, 1995.10) embryonic/larval cuticle (Meister and Braun, 1995.10) epidermis (Meister and Braun, 1995.10) embryonic/larval somatic muscle (Meister and Braun, 1995.10) embryonic/larval salivary gland (Meister and Braun, 1995.10) Malpighian tubule (Meister and Braun, 1995.10) embryonic/larval hindgut (Meister and Braun, 1995.10)
Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)j5B5 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	12151 y¹ w^*; P{lacW}how^j5B5/TM3, Sb¹
Kyoto	111421 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}82B P{lacW}how^j5B5/TM6B, P{Car20y}TPN1, Tb¹
Linkouts
Comments
	Location 3R:17867954-17867955 confirmed by FlyBase alignment of dbGSS accession AQ034089 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P2151 (Meister and Braun, 1995.10) P{lacW}how^j5B5 (FlyBase, 2005, FlyBase, 1992-) P{lacW}l(3)j5B5^j5B5 (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{w^+mC=lacW}l(3)j5B5^j5B5 (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0001186
References ( 10 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material	Parks et al., 2004, Nature Genetics 36(3): Supplementary Table 1: Deletions generated at Bloomington between trans-heterozygous P element insertions. [FBrf0179070]
Personal communication to FlyBase	Andrade et al., 2003.5.20, Isolation and Characterization of Df(3R)BSC34. Isolation and Characterization of Df(3R)BSC34. [FBrf0159866] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}howj5B5 Insertion

Dmel\P{lacW}how^j5B5 Insertion