A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{lacW}smidj6B8 Insertion

General Information
Symbol Dmel\P{lacW}smidj6B8 Species D. melanogaster
Name FlyBase ID FBti0004987
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, smid Viability / fertility
Causes allele(s) Ecol\lacZsmid-j6B8, smidj6B8 Stock availability 2 publicly available
LINE ID l(3)j6B8
Genomic Location
Chromosomal location 3L ( 65F10 ) Sequence location 3L:7,375,041..7,375,041 [+]
Map ( GBrowse ) detailed view FBti0020059 FBti0050082 FBti0125549_1 FBti0125549_2 FBti0058259 FBti0035116 FBti0039295 FBti0110970 FBti0044109 FBti0007958 FBti0103263 FBti0105143 FBti0004987 FBti0110736 FBti0126450 FBti0025196
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:7,375,041..7,375,041 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
65F10 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
66A1-66A2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ034093
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999)
smid
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZsmid-j6B8
(BDGP Project Members, 1994-1999)
smidj6B8
(BDGP Project Members, 1994-1999, Spradling et al., 1999)
Lethality
References
lethal | recessive
(BDGP Project Members, 1994-1999, Spradling et al., 1999)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
In wandering third instar larva, no detectable β-gal staining.
(Meister and Braun, 1995.10)
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)j6B8
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
12070
y1 w*; P{lacW}smidj6B8/TM3, Sb1
Kyoto
111382
yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}smidj6B8 P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb1
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hide Comments
Location 3L:7355888-7355889 confirmed by FlyBase alignment of dbGSS accession AQ034093 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P2070
(Meister and Braun, 1995.10)
P{lacW}l(3)j6B8j6B8
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{lacW}smidj6B8
(FlyBase, 2005, FlyBase, 1992-)
P{w+mC=lacW}l(3)j6B8j6B8
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0001192
hide References ( 8 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]