Dmel\P{lacW}Stat92E^j6C8 Insertion

General Information
Symbol	Dmel\P{lacW}Stat92E^j6C8	Species	D. melanogaster
Name		FlyBase ID	FBti0004990
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, Stat92E	Viability / fertility
Causes allele(s)	Ecol\lacZ^Stat92E-j6C8, Stat92E^j6C8	Stock availability	none publicly available
LINE ID	l(3)j6C8
Genomic Location
Chromosomal location	3R ( 92F1 )	Sequence location
Member of Large Scale Dataset(s)
Dataset	TI_set_P{lacW}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800). Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	92F1 ( near gene of known cytology )
Cytological location (reported)	92E1-92E2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) Stat92E (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^Stat92E-j6C8 (BDGP Project Members, 1994-1999) Stat92E^j6C8 (BDGP Project Members, 1994-1999, Reynolds-Kenneally and Mlodzik, 2005, Spradling et al., 1999, Zeidler et al., 1999, Read et al., 2004)

Lethality References lethal (Singh et al., 2010) lethal \| embryonic stage (Ekas et al., 2010) lethal \| first instar larval stage (Ekas et al., 2010) lethal \| recessive (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Sterility References
Phenotype Manifest In
	antenna \| somatic clone (Read et al., 2004) cyst cell \| heat sensitive (Singh et al., 2010) cyst progenitor cell \| male \| heat sensitive (Singh et al., 2010) cyst progenitor cell \| male \| somatic clone (Singh et al., 2010) egg chamber (Torres et al., 2003) egg chamber \| somatic clone (McGregor et al., 2002) eye \| somatic clone (Read et al., 2004) follicle cell \| somatic clone (Torres et al., 2003) germarium \| cell non-autonomous \| somatic clone (Decotto and Spradling, 2005) interfollicle cell \| anterior \| cell autonomous (Torres et al., 2003) male germline stem cell \| germline clone (Tulina and Matunis, 2001) male germline stem cell \| heat sensitive (Singh et al., 2006, Singh et al., 2010) oocyte (Torres et al., 2003) ovarian basement membrane \| cell non-autonomous \| somatic clone (Decotto and Spradling, 2005) ovarian sheath cell \| cell non-autonomous \| somatic clone (Decotto and Spradling, 2005) polar follicle cell (Torres et al., 2003) spermatocyte \| heat sensitive (Singh et al., 2006) spermatogonium \| heat sensitive (Singh et al., 2006)
Detailed Description
Statement Reference 24% of Stat92E[j6C8]/Df(3R)H-B79 animals die during embryogenesis and the remainder die during the first larval instar. (Ekas et al., 2010) Expression of Stat92E[Scer\UAS.cSa] under the control of Scer\GAL4[C587] rescues the loss of somatic cyst progenitor cells that is seen after 4 days at 29[o]C in the testes of Stat92E[F]/Stat92E[j6C8] males (raised at 22[o]C). The rescued animals also have wild-type somatic cyst cells and most germline stem cells are also restored. (Singh et al., 2010) Marked homozygous somatic cyst progenitor cell clones in the testis are extremely unstable compared to wild-type control clones. Somatic cyst progenitor cells (CPCs) and germline stem cells are completely lost and the hub moves to the inside in most testes after 4 days at 29[o]C in Stat92E[F]/Stat92E[j6C8] males (raised at 22[o]C). 70% of the testes retain some somatic cyst cells. Marker analysis indicates that the CPCs differentiate into daughter somatic cyst cells in the mutant testes at 29[o]C. When the flies are shifted from 29[o]C back to 18[o]C for 3 days, marker analysis suggests that the daughter somatic cyst cells are able to de-differentiate back into CPCs. (Singh et al., 2010) Stat92E[F]/Stat92E[j6C8] males expressing Socs36E[Scer\UAS.P\T.cCa] under the control of Scer\GAL4[C587] show complete loss of somatic cyst progenitor cells, somatic cyst cells, germline stem cells, spermatogonia and spermatocytes after 2 days at 29[o]C. (Singh et al., 2010) Hyperplasia in adult Malpighian tubule clones expressing Ras85D[V12.Scer\UAS] (using the MARCM system, under the control of Scer\GAL80[αTub84B.PL] and Scer\GAL4[Scer\FRT.Act5C]) is not suppressed by making them also mutant for Stat92E[j6C8]. (Zeng et al., 2010) After shifting to the restrictive temperature of 29[o]C, germline stem cells, spermatogonia and spermatocytes are gradually lost over time in Stat92E[F]/Stat92E[j6C8] testes. After 4 days at 29[o]C, germline stem cells are completely lost and only a fraction of testes retain spermatogonia. After 7 days at 29[o]C, germline stem cells, spermatogonia and spermatocytes are completely lost in all Stat92E[F]/Stat92E[j6C8] testes. (Singh et al., 2006) Expression of BHD[Scer\UAS.cSa] under the control of Scer\GAL4[nos.PG] significantly slows down the loss of germ cell phenotype that is seen in Stat92E[F]/Stat92E[j6C8] testes at 29[o]C. After 4 days at 29[o]C, 26% of the double mutant testes contain one or more germline stem cells and most of the testes retain spermatogonia. After 7 days at 29[o]C, 8% of the double mutant testes have one or more germline stem cells and 64% retain spermatogonia. Expression of os[Scer\UAS.cCa] under the control of Scer\GAL4[nos.PG] does not suppress the loss of germ cell phenotype that is seen in Stat92E[F]/Stat92E[j6C8] testes at 29[o]C. Expression of dpp[Scer\UAS.cUa] under the control of Scer\GAL4[nos.PG] significantly slows down the loss of germ cell phenotype that is seen in Stat92E[F]/Stat92E[j6C8] testes at 29[o]C. After 4 days at 29[o]C, 41% of the double mutant testes contain one or more germline stem cells and all of the testes retain spermatogonia. After 7 days at 29[o]C, 17% of the double mutant testes have one or more germline stem cells and 88% retain spermatogonia. (Singh et al., 2006) Stat92E^j6C8 homozygous female germ line clones develop normally. Early oogenesis is unaffected when follicle stem cells are homozygous for Stat92E^j6C8 (as somatic clones) but cysts containing mutant follicle cells frerquently do not become encapsulated. In contarst, when escort cells are homozygous for Stat92E^j6C8 (as somatic clones), germaria become distorted close to the mutant cells, lack normal patterns of developing cysts, and are suurounded by an expanded muscle sheath and associated basement membrane. Numbers of germline stem cells are reduced in these germaria and mislocalised spectrosomes. (Decotto and Spradling, 2005) Heterozygosity for Stat92E^j6C8 fails to suppress the large eye phenotype of both Scer\GAL4^ey.PH > Ser^Scer\UAS.cSa and Scer\GAL4^ey.PH > Dl^Scer\UAS.cHa flies. (Reynolds-Kenneally and Mlodzik, 2005) When homozygous mutant eyes are made using somatic clones, the resultant eyes are mostly normal showing a slight reduction in size, some misaligned ommatidia and infrequently, missing antennal structures. (Read et al., 2004) The enlarged eye phenotype seen in flies carrying one copy of os^GMR.PB is strongly suppressed by one copy of Stat92E^j6C8. (Bach et al., 2003) Follicle cell clones of Stat92E^j6C8 cause a cell-autonomous loss-of-stalk fate. When such Stat92E^j6C8 clones prevent the formation of the anterior stalk of an cyst, this chamber fuses with the adjacent anterior cyst. The oocyte of the anterior egg chamber is then mispositioned. In contrast, when all of the epithelial follicle cells and the polar cells are Stat92E^j6C8 clones, but the stalk cells are wild-type, the oocyte is positioned correctly. (Torres et al., 2003) Females with homozygous clones in the follicle cells show egg chamber fusions. (McGregor et al., 2002) Homozygous germ line stem cells are frequently detected in the testis two days after clone induction. However, the percentage of testes containing germ line stem cell clones decreases over time and they are absent by 5 days after clone induction. (Tulina and Matunis, 2001)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)j6C8 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu). (FlyBase, 1992-)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}Stat92E^j6C8 (BDGP Project Members, 1994-1999, FlyBase, 1992-, ) STAT92E^J6C8 (Reynolds-Kenneally and Mlodzik, 2005)
Secondary FlyBase IDs

References ( 19 )
Research paper	Ekas et al., 2010, Dev. Biol. 344(2): 621--636 Characterization of a dominant-active STAT that promotes tumorigenesis in Drosophila. [FBrf0211400] Singh et al., 2010, J. Cell. Physiol. 223(2): 500--510 Competitiveness for the niche and mutual dependence of the germline and somatic stem cells in the Drosophila testis are regulated by the JAK/STAT signaling. [FBrf0210092] Zeng et al., 2010, J. Cell. Physiol. 224(3): 766--774 Tumor suppressors Sav/scrib and oncogene ras regulate stem-cell transformation in adult Drosophila malpighian tubules. [FBrf0211181] Singh et al., 2006, Oncogene 25(44): 5933--5941 The Drosophila homolog of the human tumor suppressor gene BHD interacts with the JAK-STAT and Dpp signaling pathways in regulating male germline stem cell maintenance. [FBrf0194250] Decotto and Spradling, 2005, Dev. Cell 9(4): 501--510 The Drosophila ovarian and testis stem cell niches: similar somatic stem cells and signals. [FBrf0190200] Reynolds-Kenneally and Mlodzik, 2005, Dev. Biol. 285(1): 38--48 Notch signaling controls proliferation through cell-autonomous and non-autonomous mechanisms in the Drosophila eye. [FBrf0187397] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Read et al., 2004, Mol. Cell. Biol. 24(15): 6676--6689 Drosophila C-terminal Src kinase negatively regulates organ growth and cell proliferation through inhibition of the Src, Jun N-terminal kinase, and STAT pathways. [FBrf0179404] Bach et al., 2003, Genetics 165(3): 1149--1166 A sensitized genetic screen to identify novel regulators and components of the Drosophila janus kinase/signal transducer and activator of transcription pathway. [FBrf0167623] Torres et al., 2003, Dev. Cell 5(4): 547--558 A Notch/Delta-dependent relay mechanism establishes anterior-posterior polarity in Drosophila. [FBrf0167471] McGregor et al., 2002, Development 129(3): 705--717 JAK signaling is somatically required for follicle cell differentiation in Drosophila. [FBrf0144841] Tulina and Matunis, 2001, Science 294(5551): 2546--2549 Control of stem cell self-renewal in Drosophila spermatogenesis by JAK-STAT signaling. [FBrf0141754] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Zeidler et al., 1999, Genes Dev. 13(10): 1342--1353 Polarity determination in the Drosophila eye: a novel role for unpaired and JAK/STAT signaling. [FBrf0109174] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lacW}Stat92Ej6C8 Insertion

Dmel\P{lacW}Stat92E^j6C8 Insertion