Dmel\P{lacW}scrib^j7B3 Insertion

General Information
Symbol	Dmel\P{lacW}scrib^j7B3	Species	D. melanogaster
Name		FlyBase ID	FBti0004997
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, scrib	Viability / fertility
Causes allele(s)	Ecol\lacZ^scrib-j7B3, scrib^j7B3	Stock availability	2 publicly available
LINE ID	l(3)j7B3
Genomic Location
Chromosomal location	3R ( 97B9 )	Sequence location	3R:22,364,905..22,364,905 [+]
Map ( GBrowse )
Recent Updates
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	3R:22,364,905..22,364,905 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	97B9 ( inferred by FlyBase from sequence location )
Cytological location (reported)	97B8-97B9 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ034098 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) scrib (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^scrib-j7B3 (BDGP Project Members, 1994-1999) scrib^j7B3 (BDGP Project Members, 1994-1999, Li et al., 2001, Bilder and Perrimon, 2000, Ganguly et al., 2003, Spradling et al., 1999)

Lethality References lethal \| larval stage (Dow et al., 2003) lethal \| recessive (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Sterility References
Phenotype Manifest In
	egg \| maternal effect (Cook and Carpenter, 2002) wing disc (Zeitler et al., 2004) wing disc \| third instar larval stage (Dow et al., 2003)
Detailed Description
Statement Reference The trachea of sinu^nwu7 scrib^j7B3 double mutants have the same phenotype as sinu^nwu7 single mutants. (Wu et al., 2004) Expression of scrib^{ΔLRR.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^69B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib⁶⁷³/scrib^j7B3 transheterozygotes. Expression of scrib^{ΔLRR.Scer\UAS.P\T.T:Myr6,T:Avic\GFP} under the control of Scer\GAL4^69B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib⁶⁷³/scrib^j7B3 transheterozygotes. Expression of scrib^{4.Scer\UAS.P\T.T:Avic\GFP} or scrib^{ΔPDZs.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^69B provides a more complete rescue of the size phenotype of scrib⁶⁷³/scrib^j7B3 transheterozygous discs compared to scrib null discs. Increasing the level of transgene expression via raising larvae at 29 ^oC further increases this rescue. (Zeitler et al., 2004) Wing discs from larvae homozygous for scrib^j7B3 show normal epithelial organisation but are increased in size compared to wild type. (Zeitler et al., 2004) Ubiquitous expression of Hsap\Scrib[Scer\UAS.T:Avic\GFP-EGFP] (under the control of Scer\GAL4[Act.PU]) effectively suppresses the larval overgrowth phenotype found in scrib[1]/scrib[j7B3] mutants. Expression of Hsap\Scrib[Scer\UAS.T:Avic\GFP-EGFP] in scrib[1]/scrib[j7B3] mutant third instar imaginal wings suppresses the tumorous overgrowth of the epithelial tissues and allows the formation of a monolayered epithelial structure closely resembling that of wild-type discs. Expression of Hsap\Scrib[Scer\UAS.T:Avic\GFP-EGFP] (under the control of Scer\GAL4[Act.PU]) rescues larval lethality in scrib[1]/scrib[j7B3] mutants to allow 59% to form adult structures (pharate adults) and 23% to eclose as mature adult flies. These adult flies exhibit some developmental defects in the eyes and wings on a proportion of rescued animals as well as ectopic scutellar bristles, suggesting the rescue effect is not fully penetrant. Both sexes appear to be sterile. (Dow et al., 2003) Less than 9% of scrib[1]/scrib[j7B3] mutant larvae survive to pupation. At the third instar stage of larval development, scrib[1]/scrib[j7B3] mutants fail to pupate and continue to grow to form giant larvae. The third instar imaginal wings taken from scrib[1]/scrib[j7B3] mutants show loss of monolayered structure and growth in three dimensions. (Dow et al., 2003) The avoidance response to benzaldehyde of scrib^j7B3/scrib^smi97B adults is not significantly different from that of controls. (Ganguly et al., 2003) scrib^j7B3/T(Y;3)G75 females lay eggs that do not hatch. (Cook and Carpenter, 2002) Some scrib⁸⁸²/scrib^j7B3 animals survive to adulthood. (Wu et al., 2001)
Expression Data
Reporter Expression

Additional Information
Statement Reference In wandering third instar larva, no detectable β-gal staining. (Meister and Braun, 1995.10)

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)j7B3 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	12159 y¹ w^*; P{lacW}scrib^j7B3/TM3, Sb¹
Kyoto	111422 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}82B P{lacW}scrib^j7B3/TM6B, P{Car20y}TPN1, Tb¹
Linkouts
Comments
	Location 3R:22364905-22364906 confirmed by FlyBase alignment of dbGSS accession AQ034098 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P2159 (Meister and Braun, 1995.10) P{lacW}l(3)j7B3^j7B3 (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{lacW}scrib^j7B3 (FlyBase, 2005, FlyBase, 1992-) P{w^+mC=lacW}l(3)j7B3^j7B3 (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0001198
References ( 16 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Wu et al., 2004, J. Cell Biol. 164(2): 313--323 Sinuous is a Drosophila claudin required for septate junction organization and epithelial tube size control. [FBrf0167773] Zeitler et al., 2004, J. Cell Biol. 167(6): 1137--1146 Domains controlling cell polarity and proliferation in the Drosophila tumor suppressor Scribble. [FBrf0184060] Dow et al., 2003, Oncogene 22: 9225--9230 hScrib is a functional homologue of the Drosophila tumour suppressor Scribble. [FBrf0185149] Ganguly et al., 2003, Genetics 164(4): 1447--1457 Scribble is essential for olfactory behavior in Drosophila melanogaster. [FBrf0162145] Cook and Carpenter, 2002, D. I. S. 85: 34--37 Characterization of Tp(3;3)ME61 and genetic observations on chromosomal regions 96 and 97 of Drosophila melanogaster. [FBrf0179696] Li et al., 2001, Mech. Dev. 108(1-2): 185--190 Differential expression of two scribble isoforms during Drosophila embryogenesis. [FBrf0139743] Wu et al., 2001, Genetics 159(1): 189--199 Drosophila immunity. Genes on the third chromosome required for the response to bacterial infection. [FBrf0138410] Bilder and Perrimon, 2000, Nature 403(6770): 676--680 Localization of apical epithelial determinants by the basolateral PDZ protein Scribble. [FBrf0125422] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase	Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}scribj7B3 Insertion

Dmel\P{lacW}scrib^j7B3 Insertion