A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{lacW}l(3)j8B6j8B6 Insertion

General Information
Symbol Dmel\P{lacW}l(3)j8B6j8B6 Species D. melanogaster
Name FlyBase ID FBti0005004
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data
Affected gene(s) Ecol\lacZ, l(3)j8B6 Viability / fertility
Causes allele(s) Ecol\lacZj8B6, l(3)j8B6j8B6 Stock availability 1 publicly available
LINE ID l(3)j8B6
Genomic Location
Chromosomal location 3R ( 86B1 ) Sequence location 3R:6,182,225..6,182,225 [+]
Map ( GBrowse ) detailed view FBti0019353 FBti0054522 FBti0115177 FBti0005555 FBti0029242 FBti0126022 FBti0034403 FBti0113348 FBti0040133 FBti0059000 FBti0048459 FBti0129381 FBti0005550 FBti0037494 FBti0005004 FBti0007805 FBti0016472 FBti0099906 FBti0055534 FBti0050883 FBti0053264 FBti0046055 FBti0047961 FBti0045632 FBti0043345 FBti0045610
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Description
What does this section display?
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This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:6,182,225..6,182,225 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
86B1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
86B1-86B2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026332
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999)
l(3)j8B6
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZj8B6
(BDGP Project Members, 1994-1999)
l(3)j8B6j8B6
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Vernooy et al., 2002)
Lethality
References
lethal | recessive
(Spradling et al., 1999, BDGP Project Members, 1994-1999)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
When l(3)j8B6j8B6 is added to WGMR.PH, grimGMR.PH or rprGMR.PH no effect is seen on the cell death phenotype in the eye.
(Vernooy et al., 2002)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)j8B6
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
10238
y1 w1118; P{lacW}l(3)j8B6j8B6/TM3, Sb1
hide Linkouts
hide Comments
Location 3R:6182225-6182226 confirmed by FlyBase alignment of dbGSS accession AQ026332 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.
(FlyBase, 2005)
The insertion sites of P{Mae-GMR.E}BruceGMREP-86a-3, P{Mae-GMR.E}BruceGMREP-86a-4, P{Mae-GMR.E}BruceGMREP-86a-5, P{EP}stich1EP359, P{EP}jumuEP739, P{PZ}jumu06142 and P{lacW}l(3)j8B6j8B6 are within 6 base pairs of each other.
(Vernooy et al., 2002)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}l(3)j8B6j8B6
(FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 8 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Vernooy et al., 2002, Curr. Biol. 12(13): 1164--1168
Drosophila bruce can potently suppress rpr- and grim-dependent but not hid-dependent cell death. [FBrf0151242]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]