Dmel\P{lacW}l(3)01239^j9B4 Insertion

General Information
Symbol	Dmel\P{lacW}l(3)01239^j9B4	Species	D. melanogaster
Name		FlyBase ID	FBti0005011
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, l(3)01239	Viability / fertility
Causes allele(s)	Ecol\lacZ^j9B4, l(3)01239^j9B4	Stock availability	1 publicly available
LINE ID	l(3)j9B4
Genomic Location
Chromosomal location	3L ( 68A4 )	Sequence location	3L:11,067,448..11,067,448 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:11,067,448..11,067,448 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	68A4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	68A4-68A5 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ026336 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) l(3)01239 (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^j9B4 (BDGP Project Members, 1994-1999) l(3)01239^j9B4 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Makunin et al., 2002)

Lethality References lethal \| recessive (Perrimon et al., 1996, Spradling et al., 1999)
Sterility References female sterile (Perrimon et al., 1996)
Phenotype Manifest In
	oocyte \| germline clone (Perrimon et al., 1996) ovariole \| germline clone (Perrimon et al., 1996)
Detailed Description
Statement Reference Df(3L)BSC817 failed to complement female sterility of l(3)01239[j9B4]. (Christensen et al., 2009.6.15) Germline clones produce abnormal eggs due to abnormal oogenesis: no eggs laid, vitellogenic oocytes. Lethality occurs during the larval and pupal stages. (Perrimon et al., 1996)
Expression Data
Reporter Expression

Additional Information
Statement Reference In wandering third instar larva, no detectable β-gal staining. (Meister and Braun, 1995.10)

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)j9B4 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	12082 y¹ w^*; P{lacW}l(3)01239^j9B4/TM3, Sb¹
Linkouts
Comments
	Location 3L:11048295-11048296 confirmed by FlyBase alignment of dbGSS accession AQ026336 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu). (FlyBase, 1992-)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P2082 (Meister and Braun, 1995.10) P{lacW}l(3)01239^j9B4 (FlyBase, 2005, FlyBase, 1992-) P{lacW}l(3)j9B4^j9B4 (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{w^+mC=lacW}l(3)j9B4^j9B4 (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0001201
References ( 13 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Makunin et al., 2002, Genetics 160(3): 1023--1034 The Drosophila suppressor of underreplication protein binds to late-replicating regions of polytene chromosomes. [FBrf0147054] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Perrimon et al., 1996, Genetics 144(4): 1681--1692 Zygotic lethal mutations with maternal effect phenotypes in Drosophila melanogaster. [FBrf0091142] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material	Parks et al., 2004, Nature Genetics 36(3): Supplementary Table 1: Deletions generated at Bloomington between trans-heterozygous P element insertions. [FBrf0179070]
Personal communication to FlyBase	Christensen et al., 2009.6.15, Isolation and characterization of Df(3L)BSC817. Isolation and characterization of Df(3L)BSC817. [FBrf0208101] Deal and Cook, 2002.2.24, Isolation and characterization of Df(3L)BSC14. Isolation and characterization of Df(3L)BSC14. [FBrf0145645] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}l(3)01239j9B4 Insertion

Dmel\P{lacW}l(3)01239^j9B4 Insertion