A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{hsneo}l(3)neo191 Insertion

General Information
Symbol Dmel\P{hsneo}l(3)neo191 Species D. melanogaster
Name FlyBase ID FBti0005026
Feature type transposable_element_insertion_site
Description
Inserted element P{hsneo} Expression data
Affected gene(s) l(3)neo19 Viability / fertility
Causes allele(s) l(3)neo191 Stock availability 1 publicly available
LINE ID l(3)neo19
Genomic Location
Chromosomal location 3L ( 70A8 ) Sequence location 3L:13,405,975..13,405,975 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0037520 FBti0124383 FBti0066087 FBti0077114 FBti0039014 FBti0027382 FBti0113256 FBti0005026 FBti0035203 FBti0099723 FBti0048143 FBti0052116 FBti0101665 FBti0066710
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{hsneo}; most lines have a lethal or sterile phenotype. The P{hsneo} construct carries a neomycin-resistance gene with a heat-shock promoter (allowing chemical selection in flies using G418), and bacterial sequences that allow plasmid rescue. First dataset of this type.
Insertion lines from this collection were assessed for inclusion in the BDGP Gene Disruption Project collection.
(Cooley et al., 1988, Spradling et al., 1999)
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Description
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What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Chromosome (arm)
Sequence Location
3L:13,405,975..13,405,975 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
70A8 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
70A1-70A8 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026338
(FlyBase, 1992-,  
hide Inserted Element
Construct P{hsneo}
Location-dependent
role
Size 5.075Kb
Associated alleles
Tn\neoRHsp70Bb.PS
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
l(3)neo19
(Cooley et al., 1988)
hide Alleles and Phenotypes
Causes alleles
l(3)neo191
(Cooley et al., 1988, Spradling et al., 1999, Nolo et al., 2000, Nolo et al., 2001)
Lethality
References
lethal | recessive
(Cooley et al., 1988, Spradling et al., 1999)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)neo19
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
10257
mwh1 P{hsneo}l(3)neo191 red1 e1/TM3, ryRK Sb1 Ser1
hide Linkouts
hide Comments
Location 3L:13386822-13386823 confirmed by FlyBase alignment of dbGSS accession AQ026338 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P257
(Nolo et al., 2001)
P{hsneo}l(3)neo191
(FlyBase, 2005, Cooley et al., 1988, FlyBase, 1992-, )
P{l(3)neo19}
(Nolo et al., 2000)
Secondary FlyBase IDs
  • FBti0000068
hide References ( 10 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Nolo et al., 2001, Genetics 157(1): 307--315
Drosophila Lyra mutations are gain-of-function mutations of senseless. [FBrf0132337]
Nolo et al., 2000, Cell 102(3): 349--362
Senseless, a Zn finger transcription factor, is necessary and sufficient for sensory organ development in Drosophila. [FBrf0129996]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Cooley et al., 1988, Science 239: 1121--1128
Insertional mutagenesis of the Drosophila genome with single P-elements. [FBrf0049003]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]