Dmel\P{hsneo}l(3)neo27¹ Insertion

General Information
Symbol	Dmel\P{hsneo}l(3)neo27¹	Species	D. melanogaster
Name		FlyBase ID	FBti0005033
Feature type	transposable_element_insertion_site
Description
Inserted element	P{hsneo}	Expression data
Affected gene(s)	l(3)neo27	Viability / fertility
Causes allele(s)	l(3)neo27¹	Stock availability	1 publicly available
LINE ID	l(3)neo27
Genomic Location
Chromosomal location	3L ( 76A1 )	Sequence location	3L:19,163,739..19,163,739 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{hsneo}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{hsneo}; most lines have a lethal or sterile phenotype. The P{hsneo} construct carries a neomycin-resistance gene with a heat-shock promoter (allowing chemical selection in flies using G418), and bacterial sequences that allow plasmid rescue. First dataset of this type. Insertion lines from this collection were assessed for inclusion in the BDGP Gene Disruption Project collection. (Cooley et al., 1988, Spradling et al., 1999)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:19,163,739..19,163,739 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	76A1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	76A1-76A7 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ026341 (FlyBase, 1992-,
Inserted Element
Construct	P{hsneo}
Location-dependent role
Size	5.075Kb
Associated alleles	Tn\neoR^Hsp70Bb.PS
Molecular map
Affected Gene(s)
Insertion may affect gene	l(3)neo27 (Cooley et al., 1988)
Alleles and Phenotypes
Causes alleles	l(3)neo27¹ (Cooley et al., 1988, Spradling et al., 1999)

Lethality References lethal \| recessive (Spradling et al., 1999, Cooley et al., 1988)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)neo27 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	12223 mwh¹ P{hsneo}l(3)neo27¹ red¹ e¹/TM3, ry^RK Sb¹ Ser¹
Linkouts
Comments
	Location 3L:19135898-19135899 confirmed by FlyBase alignment of dbGSS accession AQ026341 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{hsneo}l(3)neo27¹ (FlyBase, 2005, Cooley et al., 1988, FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 8 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Cooley et al., 1988, Science 239: 1121--1128 Insertional mutagenesis of the Drosophila genome with single P-elements. [FBrf0049003]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{hsneo}l(3)neo271 Insertion

Dmel\P{hsneo}l(3)neo27¹ Insertion