A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{hsneo}l(3)neo381 Insertion

General Information
Symbol Dmel\P{hsneo}l(3)neo381 Species D. melanogaster
Name FlyBase ID FBti0005041
Feature type transposable_element_insertion_site
Description
Inserted element P{hsneo} Expression data
Affected gene(s) l(3)neo38 Viability / fertility
Causes allele(s) l(3)neo381 Stock availability 1 publicly available
LINE ID l(3)neo38
Genomic Location
Chromosomal location 3R ( 86E18 ) Sequence location 3R:7,603,634..7,603,634 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0107095 FBti0105651 FBti0021674 FBti0102567 FBti0109410 FBti0005607 FBti0099765 FBti0111904 FBti0106369 FBti0069086 FBti0028140 FBti0068649 FBti0021417 FBti0043501 FBti0064857 FBti0044406 FBti0036083 FBti0131578 FBti0047123 FBti0005041 FBti0100772 FBti0069677 FBti0070573 FBti0036227 FBti0055160 FBti0034080 FBti0100345 FBti0029737 FBti0029571 FBti0055707 FBti0105759 FBti0108955 FBti0103143 FBti0126541 FBti0044918 FBti0024783 FBti0007998 FBti0112347 FBti0126042_1 FBti0052647 FBti0047400 FBti0052368 FBti0126042_2 FBti0075484 FBti0041587 FBti0066196 FBti0045848 FBti0129415 FBti0130100
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{hsneo}; most lines have a lethal or sterile phenotype. The P{hsneo} construct carries a neomycin-resistance gene with a heat-shock promoter (allowing chemical selection in flies using G418), and bacterial sequences that allow plasmid rescue. First dataset of this type.
Insertion lines from this collection were assessed for inclusion in the BDGP Gene Disruption Project collection.
(Cooley et al., 1988, Spradling et al., 1999)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:7,603,634..7,603,634 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
86E18 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
86F1-86F11 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ034103
(FlyBase, 1992-,  
hide Inserted Element
Construct P{hsneo}
Location-dependent
role
Size 5.075Kb
Associated alleles
Tn\neoRHsp70Bb.PS
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
l(3)neo38
(Cooley et al., 1988, Spradling et al., 1999, Levis, 2003.3.6)
hide Alleles and Phenotypes
Causes alleles
l(3)neo381
(Cooley et al., 1988, Spradling et al., 1999, Levis, 2003.3.6)
Lethality
References
lethal | recessive
(Spradling et al., 1999, Cooley et al., 1988)
partially lethal - majority die | recessive
(Cooley and Spradling, 1990.8.1)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)neo38
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
10275
mwh1 P{hsneo}l(3)neo381 red1 e1/TM3, ryRK Sb1 Ser1
hide Linkouts
hide Comments
Location 3R:7603634-7603635 confirmed by FlyBase alignment of dbGSS accession AQ034103 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{hsneo}l(3)neo381
(FlyBase, 2005, Cooley et al., 1988, FlyBase, 1992-, )
Secondary FlyBase IDs
  • FBti0000085
hide References ( 10 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Cooley et al., 1988, Science 239: 1121--1128
Insertional mutagenesis of the Drosophila genome with single P-elements. [FBrf0049003]
Personal communication to FlyBase
Levis, 2003.3.6, P{hsneo}l(3)neo38<up>1</up> is inserted in the CG31364 gene.
P{hsneo}l(3)neo381 is inserted in the CG31364 gene. [FBrf0155402]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
Cooley and Spradling, 1990.8.1, Insertions and alleles.
Insertions and alleles. [FBrf0122639]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]