Dmel\P{hsneo}Hsrω^neo55 Insertion

General Information
Symbol	Dmel\P{hsneo}Hsrω^neo55	Species	D. melanogaster
Name		FlyBase ID	FBti0005054
Feature type	transposable_element_insertion_site
Description
Inserted element	P{hsneo}	Expression data
Affected gene(s)	Hsrω	Viability / fertility
Causes allele(s)	Hsrω^neo55	Stock availability	none publicly available
LINE ID	l(3)neo55
Genomic Location
Chromosomal location	3R ( 93D4 )	Sequence location	3R:17,122,223..17,122,223 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{hsneo}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{hsneo}; most lines have a lethal or sterile phenotype. The P{hsneo} construct carries a neomycin-resistance gene with a heat-shock promoter (allowing chemical selection in flies using G418), and bacterial sequences that allow plasmid rescue. First dataset of this type. Insertion lines from this collection were assessed for inclusion in the BDGP Gene Disruption Project collection. (Cooley et al., 1988, Spradling et al., 1999)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3R:17,122,223..17,122,223 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	93D4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	93D1-93D10 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ026350
Inserted Element
Construct	P{hsneo}
Location-dependent role
Size	5.075Kb
Associated alleles	Tn\neoR^Hsp70Bb.PS
Molecular map
Affected Gene(s)
Insertion may affect gene	Hsrω (Cooley et al., 1988)
Alleles and Phenotypes
Causes alleles	Hsrω^neo55 (Cooley et al., 1988, Fernandez-Funez et al., 2000)

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference The rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is not modified if the flies are also carrying Hsrω^neo55. (Shulman and Feany, 2003) Expression of Hsrω^neo55 under the control of Scer\GAL4^GMR.PF does not result in a rough eye phenotype. (Shulman and Feany, 2003)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)neo55 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	Location 3R:17122223-17122224 confirmed by FlyBase alignment of dbGSS accession AQ026350 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. (FlyBase, 2005) This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu). (FlyBase, 1992-)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P292 (Fernandez-Funez et al., 2000) P{hsneo}Hsrω^neo55 (FlyBase, 2005, FlyBase, 1992-, ) P{hsneo}l(3)neo55¹ (Cooley et al., 1988, FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000100
References ( 10 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Shulman and Feany, 2003, Genetics 165(3): 1233--1242 Genetic modifiers of tauopathy in Drosophila. [FBrf0167629] Fernandez-Funez et al., 2000, Nature 408(6808): 101--106 Identification of genes that modify ataxin-1-induced neurodegeneration. [FBrf0131287] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Cooley et al., 1988, Science 239: 1121--1128 Insertional mutagenesis of the Drosophila genome with single P-elements. [FBrf0049003]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{hsneo}Hsrωneo55 Insertion

Dmel\P{hsneo}Hsrω^neo55 Insertion