A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{PZ}Rcd5rG166 Insertion

General Information
Symbol Dmel\P{PZ}Rcd5rG166 Species D. melanogaster
Name FlyBase ID FBti0005061
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, Rcd5 Viability / fertility
Causes allele(s) Ecol\lacZrG166, Rcd5rG166 Stock availability 1 publicly available
LINE ID l(3)rG166
Genomic Location
Chromosomal location 3L ( 64A4 ) Sequence location 3L:4,042,236..4,042,236 [+]
Map ( GBrowse ) detailed view FBti0075706 FBti0126405 FBti0115964 FBti0042836 FBti0064683 FBti0066402 FBti0005061
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Description
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FB2012_01
Controlled Vocabulary Terms
third instar larval stage
References
Andersen et al., 2010, Mol. Cell. Biol. 30(19): 4744--4755
Genes
Rcd5
FB2011_10
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:4,042,236..4,042,236 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
64A4 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
64A4-64A5 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026355
(FlyBase, 1992-,  
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999)
Rcd5
(BDGP Project Members, 1994-1999, Andersen et al., 2010)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZrG166
(BDGP Project Members, 1994-1999)
Rcd5rG166
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Andersen et al., 2010)
Lethality
References
lethal | recessive
(Spradling et al., 1999, BDGP Project Members, 1994-1999)
lethal | third instar larval stage
(Andersen et al., 2010)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
The lethality found in Rcd5[rG166] mutant third instar larvae can be rescued through expression of Rcd5[Scer\UAS.cAa] under the control of Scer\GAL4[Scer\FRT.Rnor\CD2.Act5C].
(Andersen et al., 2010)
Rcd5[rG166]/Rcd5[c00114] transheterozygotes exhibit severe growth defects, and although they survive for an extended period in the third instar, they fail to pupariate. Rcd5[c07041]/Rcd5[rG166] transheterozygotes exhibit severe growth defects, and although they survive for an extended period in the third instar, they fail to pupariate.
(Andersen et al., 2010)
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage  
imaginal disc | restricted  
(Meister and Braun, 1995.10)
Additional Information
Statement
Reference
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)rG166
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
12065
P{PZ}Rcd5rG166 ry506/TM3, Sb1
hide Linkouts
hide Comments
Location 3L:4025768-4025769 confirmed by FlyBase alignment of dbGSS accession AQ026355 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P2065
(Meister and Braun, 1995.10)
P{PZ}l(3)rG166rG166
(FlyBase, 2005, BDGP Project Members, 1994-1999, Andersen et al., 2010, FlyBase, 1992-)
P{PZ}Rcd5rG166
 
P{ry+t7.2=PZ}l(3)rG166rG166
(FlyBase, 1992-)
rG166
(Andersen et al., 2010)
Secondary FlyBase IDs
  • FBti0000672
hide References ( 9 )
Research paper
Andersen et al., 2010, Mol. Cell. Biol. 30(19): 4744--4755
Drosophila MCRS2 Associates with RNA Polymerase II Complexes To Regulate Transcription. [FBrf0211794]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]