Dmel\P{PZ}wb⁰⁹⁴³⁷ Insertion

General Information
Symbol	Dmel\P{PZ}wb⁰⁹⁴³⁷	Species	D. melanogaster
Name		FlyBase ID	FBti0005158
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, wb	Viability / fertility
Causes allele(s)	Ecol\lacZ^wb-09437, wb⁰⁹⁴³⁷	Stock availability	1 publicly available
LINE ID	l(2)09437
Genomic Location
Chromosomal location	2L ( 35A3-35A4 )	Sequence location	2L:14,288,960..14,319,552 [+]
Map ( GBrowse )
Recent Updates
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:14,288,960..14,319,552 [+] (BDGP Project Members, 1994-1999, Martin et al., 1999) 2L:14,300,958..14,300,958 [+]
Orientation
Cytological location (computed by FlyBase)	35A3-35A4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	35A1-35A2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	G00417 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999, Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10) wb (BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Martin et al., 1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^wb-09437 (BDGP Project Members, 1994-1999, Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10) wb⁰⁹⁴³⁷ (BDGP Project Members, 1994-1999, Spradling et al., 1999, Ashburner et al., 1999.10.12, Ashburner et al., 1999, Martin et al., 1999, Brown and Freeman, 2003, Ashburner et al., 1999)

Lethality References lethal (Ashburner et al., 1999) lethal \| embryonic stage \| recessive (Martin et al., 1999) lethal \| recessive (Spradling et al., 1999, Ashburner et al., 1999, Roote, 1995.5.15)
Sterility References
Phenotype Manifest In
	wing (Martin et al., 1999)
Detailed Description
Statement Reference wb⁰⁹⁴³⁷ has no effect on the S⁰⁵⁶⁷¹/+ ommatidial polarity phenotype. (Brown and Freeman, 2003) S^48-5/wb⁴⁴¹⁸ mutants show 7.17%+-0.60 misrotated ommatidia compared to 9.55%+-1.43 seen in S^48-5 mutants alone. (Gaengel and Mlodzik, 2003) wb¹/wb⁰⁹⁴³⁷ flies show blistering of the wing. (Martin et al., 1999)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage epidermis \| restricted (Jackle and Janning, 1998.8.10) embryonic/larval dorsal vessel (Jackle and Janning, 1998.8.10) embryonic/larval visceral muscle (Jackle and Janning, 1998.8.10) presumptive embryonic/larval peripheral nervous system (Jackle and Janning, 1998.8.10) embryonic/larval pharynx (Jackle and Janning, 1998.8.10) anal imaginal ring (Jackle and Janning, 1998.8.10)
Additional Information
Statement Reference In embryo, expression in PNS not certain. (Jackle and Janning, 1998.8.10)

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)09437 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	12362 cn¹ P{PZ}wb⁰⁹⁴³⁷/CyO; ry⁵⁰⁶
Linkouts
Comments
	P{PZ}wb⁰⁹⁴³⁷ maps to the fourth intron of wb. The insertion maps roughly 12500 bases from the 5' end of the intron based on sequence information but the exact insertion location is unknown. (BDGP Project Members, 1994-1999, Martin et al., 1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P2362 (Meister and Braun, 1995.10) P{PZ}09437 (Ashburner et al., 1999) P{PZ}l(2)09437⁰⁹⁴³⁷ (FlyBase, 1992-) P{PZ}wb⁰⁹⁴³⁷ (FlyBase, 1992-, BDGP Project Members, 1994-1999, Martin et al., 1999) P{PZ}wb^PZ12 (FlyBase, 1992-) P{ry^+t7.2=PZ}wb⁰⁹⁴³⁷ (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000750
References ( 16 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Brown and Freeman, 2003, Development 130(22): 5401--5412 Egfr signalling defines a protective function for ommatidial orientation in the Drosophila eye. [FBrf0167498] Gaengel and Mlodzik, 2003, Development 130(22): 5413--5423 Egfr signaling regulates ommatidial rotation and cell motility in the Drosophila eye via MAPK/Pnt signaling and the Ras effector Canoe/AF6. [FBrf0167499] Ashburner et al., 1999, Genetics 153(1): 179--219 An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster. The Adh region. [FBrf0111326] Martin et al., 1999, J. Cell Biol. 145(1): 191--201 wing blister, a new Drosophila laminin alpha chain required for cell adhesion and migration during embryonic and imaginal development. [FBrf0108234] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Supplementary material	Ashburner et al., 1999, Genetics 153(1): Table S1. P-element insertions in the Adh region. [FBrf0112150]
Personal communication to FlyBase	Ashburner et al., 1999.10.12, Adh. Adh. [FBrf0112153] Jackle and Janning, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103143] Vosshall, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103142] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] Roote, 1995.5.15, PZ alleles. PZ alleles. [FBrf0080527]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{PZ}wb09437 Insertion

Dmel\P{PZ}wb⁰⁹⁴³⁷ Insertion