Dmel\P{PZ}lola⁰⁰⁶⁴² Insertion

General Information
Symbol	Dmel\P{PZ}lola⁰⁰⁶⁴²	Species	D. melanogaster
Name		FlyBase ID	FBti0005173
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, lola	Viability / fertility
Causes allele(s)	Ecol\lacZ^lola-00642, lola⁰⁰⁶⁴²	Stock availability	1 publicly available
LINE ID	l(2)00642
Genomic Location
Chromosomal location	2R ( 47A11 )	Sequence location	2R:6,421,917..6,421,917 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:6,421,917..6,421,917 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	47A11 ( inferred by FlyBase from sequence location )
Cytological location (reported)	47A11-47A12 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ073258 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Braun et al., 1997, Vosshall, 1998.8.10) lola (BDGP Project Members, 1994-1999, Paige Bass et al., 2007, Otte et al., 2009)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^lola-00642 (Braun et al., 1997, Vosshall, 1998.8.10) lola⁰⁰⁶⁴² (BDGP Project Members, 1994-1999, Zhang et al., 2003, Spradling et al., 1999, Paige Bass et al., 2007, Braun et al., 1997, Otte et al., 2009)

Lethality References lethal (Paige Bass et al., 2007, Strub et al., 2008) lethal \| recessive (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Sterility References
Phenotype Manifest In
	egg chamber \| germline clone (Paige Bass et al., 2007) nurse cell \| germline clone (Paige Bass et al., 2007)
Detailed Description
Statement Reference Females containing homozygous germline clones show defects in stage 14 egg chambers, including dumpless egg chambers (egg chambers in which the nurse cells have failed to transfer their cytoplasmic contents to the oocyte, have intact nuclei and do not appear to be undergoing programmed cell death) and egg chambers with persisting nurse cell nuclei (egg chambers in which nurse cells have transferred their cytoplasmic contents to the oocyte, but their nuclei have failed to undergo programmed cell death including nuclear condensation). Mutants have a strong mutant neuronal phenotype. (Paige Bass et al., 2007) lola⁰⁰⁶⁴²/+, SoxN^GA1192/+ double heterozygous embryos show defects in axon scaffolding, such as fusion of commissures, axonal tangles, disruption and/or reduction of longitudinal connectives. This phenotype is not seen in either single mutant heterozygote. (Girard et al., 2006) The hatch rate of lola⁰⁰⁶⁴²/+ ; JIL-1^EP3657/JIL-1^EP3657 embryos is 20%. (Zhang et al., 2003)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference wandering third instar larval stage imaginal disc (Meister and Braun, 1995.10) embryonic/larval fat body (Meister and Braun, 1995.10) epidermis (Meister and Braun, 1995.10) embryonic/larval cuticle (Meister and Braun, 1995.10) embryonic/larval somatic muscle (Meister and Braun, 1995.10) adult stage adult brain \| restricted (Vosshall, 1998.8.10) adult central complex (Vosshall, 1998.8.10)
Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)00642 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	10946 cn¹ P{PZ}lola⁰⁰⁶⁴²/CyO; ry⁵⁰⁶
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P946 (Meister and Braun, 1995.10) P{PZ}l(2)00642⁰⁰⁶⁴² (FlyBase, 1992-) P{PZ}lola⁰⁰⁶⁴² (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{ry^+t7.2=PZ}lola⁰⁰⁶⁴² (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000687
References ( 14 )
Research paper	Otte et al., 2009, D. I. S. 92: 63--69 A genetic analysis of the cytological region 46F-47B containing the Drosophila melanogaster homolog of the ELAC2 PCA susceptibility gene. [FBrf0212196] Strub et al., 2008, Genome 51(6): 409--420 Mutations of the withered (whd) gene in Drosophila melanogaster confer hypersensitivity to oxidative stress and are lesions of the carnitine palmitoyltransferase I (CPT I) gene. [FBrf0204832] Paige Bass et al., 2007, Dev. Biol.: The axon guidance gene lola is required for programmed cell death in the Drosophila ovary. [FBrf0194763] Girard et al., 2006, Dev. Biol. 299(2): 530--542 Chromatin immunoprecipitation reveals a novel role for the Drosophila SoxNeuro transcription factor in axonal patterning. [FBrf0194303] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Zhang et al., 2003, J. Biol. Chem. 278(13): 11696--11704 A developmentally regulated splice variant from the complex lola locus encoding multiple different zinc finger domain proteins interacts with the chromosomal kinase JIL-1. [FBrf0159022] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Braun et al., 1997, Genetics 147(2): 623--634 Drosophila immunity: analysis of larval hemocytes by P-element-mediated enhancer trap. [FBrf0098749] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase	Vosshall, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103142] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{PZ}lola00642 Insertion

Dmel\P{PZ}lola⁰⁰⁶⁴² Insertion