Dmel\P{PZ}ttv^00681b Insertion

General Information
Symbol	Dmel\P{PZ}ttv^00681b	Species	D. melanogaster
Name		FlyBase ID	FBti0005174
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, ttv	Viability / fertility
Causes allele(s)	Ecol\lacZ^ttv-00681b, ttv^00681b	Stock availability	1 publicly available
LINE ID	l(2)00681
Genomic Location
Chromosomal location	2R ( 51A7-51B4 )	Sequence location
Member of Large Scale Dataset(s)
Dataset	TI_set_P{PZ}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue. Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	51A7-51B4 ( near gene of known cytology )
Cytological location (reported)	51B1-51B5 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999, Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10, Russell et al., 1998) ttv (BDGP Project Members, 1994-1999, Ren et al., 2009, Baron et al., 2009, Gurudatta et al., 2010)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^ttv-00681b (BDGP Project Members, 1994-1999, Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10, Russell et al., 1998) ttv^00681b (BDGP Project Members, 1994-1999, Spradling et al., 1999, Fox and Zinn, 2005, The et al., 1999, Schulze et al., 2005, Gallet et al., 2006, Misra, 2000.8.25, Bellaiche et al., 1998, Ren et al., 2009, Baron et al., 2009, Gurudatta et al., 2010)

Lethality References lethal (Gurudatta et al., 2010, Schulze et al., 2005) lethal \| embryonic stage \| fully \| rescuable maternal effect (Perrimon et al., 1996) lethal \| pupal stage (Ren et al., 2009) lethal \| recessive (Spradling et al., 1999, Perrimon et al., 1996) viable (Schulze et al., 2005)
Sterility References
Phenotype Manifest In
	anterior fascicle & bouton (Kraut et al., 2001) bouton (Ren et al., 2009) cuticle (Perrimon et al., 1996) denticle \| germline clone \| maternal effect (Gallet et al., 2003) denticle belt (Perrimon et al., 1996) embryonic/first instar larval cuticle \| germline clone (The et al., 1999) embryonic/first instar larval cuticle \| germline clone \| maternal effect (Gallet et al., 2003) eye \| somatic clone (Amanai and Jiang, 2001) wing disc (Bellaiche et al., 1998)
Detailed Description
Statement Reference ttv[00681b] Ext2[1.8.1] double heterozygotes exhibit increased median survival time compared to controls when infected with group B Streptococcus (both wild-type and GAG-binding impaired mutants) and lower colony counts after infection. (Wang et al., 2011) ttv[00681b] Ext2[1.8.1] double heterozygotes exhibit reduced susceptibility to infection with the A909 strain of group B streptococcus. (Baron et al., 2009) Expression of ttv[Scer\UAS.T:Hsap\MYC] with Scer\GAL4[G14] leads to rescue of ttv[00681b] mutant larvae to adulthood or late pupal stages. The number of boutons per unit muscle area and bouton budding are increased to normal levels. Expression of ttv[Scer\UAS.T:Hsap\MYC] with Scer\GAL4[elav-C155] improves viability of ttv[00681b] mutant larvae. The number of boutons per unit muscle area are increased to normal levels while bouton budding is elevated above wild-type levels. Expression of ttv[Scer\UAS.T:Hsap\MYC] with Scer\GAL4[da.G32] fails to improve viability of ttv[00681b] mutant larvae but rescues the abnormally elevated mEJP frequency (but not the EJP amplitude) found in these larvae. The number of boutons relative to muscle area is significantly reduced compared with ttv[00681b] mutants. ttv[00681b] mutants exhibit morphological changes in somatic musculature, with mitochondrial distribution affected and altered Golgi organisation. (Ren et al., 2009) In response to nerve stimulation, ttv[00681b] mutants exhibit a significantly increased EJP compared with wild-type or heterozygous control larvae (20-25% increase). Spontaneous release events (mEJPs) are substantially reduced compared with heterozygous or wild-type controls, suggesting that loss of Heparan Sulfate biosynthesis affects the spontaneous vesicle release probability in the motoneuron. Post-synaptic mEJP amplitudes are significantly elevated in ttv[00681b] mutants relative to heterozygous or wild-type controls. ttv[00681b] mutant larval exhibit locomotion defects, with a significantly reduced number of peristaltic contractions per minute. ttv[00681b] mutant synapses are reduced in size and have significantly fewer boutons per unit muscle area compared with controls. ttv[00681b] motoneurons are bigger than normal, with a reduced number of \'buds\'. ttv[00681b] synapses consist primarily of large boutons without any buds. ttv[00681b] mutants exhibit an increase in activity-dependent endocytosis at the neuromuscular junction. (Ren et al., 2009) ttv^00681b mutants display ISNb bypass at a similar rate to wild-type embryos. (Fox and Zinn, 2005) Expression of hh^Scer\UAS.cIa with Scer\GAL4^en-e16E in ttv^00681b germ-line clone embryos doesn\'t rescue naked cuticle formation, but does increase denticle diversity in the ventral cuticle: denticles corresponding to row 2,3,4, and 5 types are all present. (Gallet et al., 2003) ttv^00681b germ-line clone embryos do not display any naked cuticle formation, although the resulting denticle lawn has some diversity. (Gallet et al., 2003) ttv^{Scer\UAS.T:Hsap\MYC} with Scer\GAL4^wg.PM completely rescues the cuticle phenotype of ttv^00681b germ-line clone embryos. (Gallet et al., 2003) ttv^00681b/+; trol¹³/trol¹³ larvae have increased numbers of S phase larval neuroblasts per brain lobe than wild-type. Of ttv^00681b/+; trol^p4/trol^p4 larvae, 29% show increased numbers of S phase neuroblasts per brain lobe and while 5% show decreased numbers. (Park et al., 2003) Mosaic flies with eyes homozygous for ttv^00681b (clones generated using the "eyFLP" system) have small eyes. (Amanai and Jiang, 2001) Homozygous mutant larvae have abnormally large boutons at the terminal arbor synapses of the ISN. (Kraut et al., 2001) Homozygous larvae have a marked reduction in heparan sulphate levels, to at least 10-fold less than wild type. Heterozygotes show a slight reduction in heparan sulphate levels. Chondroitin sulphate is unaffected by ttv^00681b. (Toyoda et al., 2000) ttv^00681b/Df(2R)trix embryos derived from homozygous female germline clones show absence of naked cuticle. (The et al., 1999) Clonal analysis in the wing reveals that ttv is required in the receiving cell for the movement of hh gene product from the sending to the receiving cell. (Bellaiche et al., 1998) Germline clones produce eggs with patterning defects: unrescued embryos exhibit mirror image duplication of denticle belts, rescued embryos are wild type. Lethality occurs during the larval and pupal stages. (Perrimon et al., 1996)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage embryonic/larval pharynx (Jackle and Janning, 1998.8.10) presumptive embryonic/larval central nervous system \| restricted (Jackle and Janning, 1998.8.10) presumptive embryonic/larval peripheral nervous system \| segmentally repeated (Jackle and Janning, 1998.8.10) adult stage optic lobe (Vosshall, 1998.8.10) adult brain \| restricted (Vosshall, 1998.8.10) adult central complex (Vosshall, 1998.8.10)
Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)00681 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	10949 cn¹ P{PZ}Cyt-b5^00681a P{PZ}ttv^00681b/CyO; ry⁵⁰⁶
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	00681b (Baron et al., 2009) P{PZ}l(2)00681⁰⁰⁶⁸¹ (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{PZ}ttv⁰⁰⁶⁸¹ (FlyBase, 1992-) P{PZ}ttv^00681b (FlyBase, 1992-, ) P{ry^+t7.2=PZ}l(2)00681⁰⁰⁶⁸¹ (FlyBase, 1992-) ttv^00681b (Ren et al., 2009) unnamed (Gallet et al., 2006)
Secondary FlyBase IDs
	FBti0000249
References ( 26 )

Generate a list of	All references relating to this insertion ( 26 )

List References by type	Research paper ( 18 ) Supplementary material ( 1 ) Personal communication to FlyBase ( 5 ) FlyBase analysis ( 2 )
Recent research papers ( 1 )
	Wang et al., 2011, Infect. Immun. 79(2): 606--616 Host and Pathogen Glycosaminoglycan-Binding Proteins Modulate Antimicrobial Peptide Responses in Drosophila melanogaster. [FBrf0212806] Show all research papers ...

Dmel\P{PZ}ttv00681b Insertion

Dmel\P{PZ}ttv^00681b Insertion