Dmel\P{PZ}dve⁰¹⁷³⁸ Insertion

General Information
Symbol	Dmel\P{PZ}dve⁰¹⁷³⁸	Species	D. melanogaster
Name		FlyBase ID	FBti0005198
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	dve, Ecol\lacZ	Viability / fertility
Causes allele(s)	dve⁰¹⁷³⁸, Ecol\lacZ^dve-01738	Stock availability	1 publicly available
LINE ID	l(2)01738
Genomic Location
Chromosomal location	2R ( 58D2 )	Sequence location	2R:18,158,428..18,158,435 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{PZ}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue. Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:18,158,428..18,158,435 [+] 2R:18,158,421..18,158,421 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	58D2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	58D1-58D2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ034163 (FlyBase, 1992-, G00411 (FlyBase, 1992-)
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	dve (BDGP Project Members, 1994-1999, Nakagawa et al., 2011) Ecol\lacZ (BDGP Project Members, 1994-1999, Kolzer et al., 2003, Carr et al., 2005, Vosshall, 1998.8.10, Jackle and Janning, 1998.8.10, Nakagoshi et al., 1998, Terrell et al., 2012, Ciechanska et al., 2007, Nakagawa et al., 2011, Veenstra, 2009)
Alleles and Phenotypes
Causes alleles	dve⁰¹⁷³⁸ (BDGP Project Members, 1994-1999, Carr et al., 2005, Fuss and Hoch, 1998, Nakagoshi et al., 1998, Spradling et al., 1999, Nakagawa et al., 2011) Ecol\lacZ^dve-01738 (BDGP Project Members, 1994-1999, Kolzer et al., 2003, Carr et al., 2005, Vosshall, 1998.8.10, Jackle and Janning, 1998.8.10, Nakagoshi et al., 1998, Terrell et al., 2012, Ciechanska et al., 2007, Nakagawa et al., 2011, Veenstra, 2009)

Lethality References lethal (Kolzer et al., 2003, Carr et al., 2005) lethal \| larval stage \| recessive (Nakagoshi et al., 1998, Fuss and Hoch, 1998, Nakagoshi et al., 1997) lethal \| recessive (Spradling et al., 1999)
Sterility References
Phenotype Manifest In
	cuprophilic cell (Nakagoshi et al., 1998) embryonic/larval proventriculus \| embryonic stage (Nakagoshi et al., 1998, Nakagoshi et al., 1997) joint \| ectopic \| somatic clone (Ciechanska et al., 2007) midgut (Nakagawa et al., 2011) proventriculus (Nakagawa et al., 2011) proventriculus inner layer (Fuss and Hoch, 1998) proventriculus outer layer (Fuss and Hoch, 1998) tarsal segment \| somatic clone (Ciechanska et al., 2007) wing (Kolzer et al., 2003) wing \| proximal (Kolzer et al., 2003) wing \| somatic clone (Nakagoshi et al., 2002) wing disc (Kolzer et al., 2003) wing disc \| somatic clone (Kolzer et al., 2003) wing margin bristle \| ectopic \| somatic clone (Nakagoshi et al., 2002) wing vein L2 \| proximal (Kolzer et al., 2003) wing vein L5 \| distal (Kolzer et al., 2003)
Detailed Description
Statement Reference dve[01738]/dve[NP3060] trans-heterozygotes show normal morphology and function. dve[01738]/dve[E181] and dve[01738]/Df(2R)01D01W-L186 trans-heterozygotes exhibit substantially impaired proventriculus function. dve[01738] homozygotes exhibit a reduction in copper absorption and acid secretion in the midgut. dve[01738]/dve[E181] trans-heterozygotes exhibit a milder phenotype, with dve[01738]/dve[NP3060] trans-heterozygotes milder still, while dve[01738] heterozygotes exhibit a mild reduction in copper absorption and acid secretion compared to wild-type. (Nakagawa et al., 2011) dve[01738] clones in the leg are frequently associated with ectopic joint structures in the tarsal segments, whereas clones in the femur or tibia are not associated with ectopic joints. Clones encompassing multiple tarsal segments induce extra joints in each segment, though clones spanning the joint have no effect on endogenous joint morphology. Clones spanning several joints induce only one ectopic partial joint per segment, which tends to appear in the middle of the segment. The curvature of the ectopic joints is reversed relative to the endogenous joints. No obvious effects on bristle polarity are observed. (Ciechanska et al., 2007) Wings of mutant flies are smaller and shorter than normal. The wings lack a region that encompasses most of the distal costa and a small part of the adjacent wing blade. The mutant wings are also reduced in size along the anterior-posterior axis. Wing vein 2 is interrupted in the proximal part and the distal part of wing vein 5 is missing. The size of the area of the wing outlined by wing veins 3 and 4, and the anterior crossvein and wing margin is similar in size in mutant and wild-type animals. The cell density in the area of the wing outlined by wing veins 3 and 4 is very similar in mutant and wild-type animals. The distance between wing vein 3 and 4, measured by the numbers of cells between them, is the same in mutant and wild-type animals. The cell density in the region anterior to wing vein 3 is similar in mutant and wild-type animals, but the distance between wing vein 3 and the anterior margin is reduced in mutant animals, indicating that this area consists of fewer cells in the mutant. The distance from vein 4 to the posterior wing margin is reduced in mutant animals compared to wild type, and cell density in this region is also lower than wild-type in the mutant animals, indicating that this region consists of fewer, larger cells in mutant animals compared to wild type. Mutant third larval instar wing discs are abnormal; the anlage of the dorsal part of the wing pouch is shorter than normal. In early pupae, the wing pouch is smaller than normal and has a small indentation at the anterior wing margin. The fold adjacent to the wing blade appears to be reduced in mutant wing discs. Homozygous clones induced in the wing disc are smaller than their wild-type twin clones. Clones in the regions of the disc extending from the anterior wing margin to vein 3 and from vein 4 to the posterior margin are about half the size of their wild-type counterparts. In addition, nearly half the wild-type clones in these regions do not have a mutant counterpart (suggesting that the mutant cells have died). Clones in the region from wing vein 3 to 4 are about two-thirds the size of their wild-type counterparts. (Kolzer et al., 2003) Homozygous clones in the wing result in ectopic margin bristles (which can be derived from mutant or wild-type tissue) and wing margin notches. (Nakagoshi et al., 2002) Homozygotes die as first instar larvae. Passage of food is blocked at the proventriculus in these larvae. The specification of the proventriculus primordium occurs normally in homozygous embryos. At stage 17, the endoderm outer wall structure of the proventriculus shows a collapsed phenotype and the ingrowth of the ectodermal valve cells into the endoderm fails. The number of outer wall cells is wild-type and no cell death occurs in the defective outer wall. (Fuss and Hoch, 1998) Homozygous larvae show normal locomotion behaviour immediately after hatching, however they develop into small larvae and die within a day of hatching. Food accumulates in the proventriculus, in contrast to control larvae where it is present throughout the length of the gut. The proventriculus is not correctly formed; cell migration of the foregut epithelium into the anteriormost midgut is greatly delayed and the internalisation is only temporary. Constriction of the midgut normally occurs in the midgut of homozygous embryos and the arrangement of the stage 17 midgut appears normal. The arrangement of copper cells appears disorganised in hemizygous larvae. (Nakagoshi et al., 1998) Larvae have a nonfunctional proventriculus, leading to lethality at the first instar stage. (Nakagoshi et al., 1997)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage posterior embryonic/larval midgut (Jackle and Janning, 1998.8.10) embryonic/larval brain \| presumptive (Jackle and Janning, 1998.8.10) organism \| segmentally repeated (Jackle and Janning, 1998.8.10) embryonic/larval pharynx (Jackle and Janning, 1998.8.10) presumptive embryonic/larval central nervous system \| restricted (Jackle and Janning, 1998.8.10) wandering third instar larval stage imaginal disc (Meister and Braun, 1995.10) embryonic/larval midgut (Meister and Braun, 1995.10)
Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)01738 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	11073 cn¹ P{PZ}dve⁰¹⁷³⁸/CyO; ry⁵⁰⁶
Linkouts
Comments
	8 bp insertion associated host repeat
Synonyms & Secondary IDs
Reported As
Symbol Synonym	dve¹ (Nakagawa et al., 2011) dve¹-lacZ (Nakagawa et al., 2011) dve⁰¹⁷³⁸LacZ (Terrell et al., 2012) dve-lacZ (Kolzer et al., 2003, Ciechanska et al., 2007) line 11073 (Carr et al., 2005) P1073 (Meister and Braun, 1995.10) P{PZ}^dve01738 (Veenstra, 2009) P{PZ}dve⁰¹⁷³⁸ (FlyBase, 1992-, ) P{PZ}l(2)01738⁰¹⁷³⁸ (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{PZ}l(2)dve⁰¹⁷³⁸ (Ciechanska et al., 2007) P{ry[+t7.2]=PZ}dve[01738] (Carr et al., 2005) P{ry^+t7.2=PZ}l(2)01738⁰¹⁷³⁸ (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000276
References ( 20 )
Research paper	Terrell et al., 2012, Dev. Dyn. 241(1): 215--228 OTX2 and CRX rescue overlapping and photoreceptor-specific functions in the Drosophila eye. [FBrf0216943] Nakagawa et al., 2011, Mech. Dev. 128(5-6): 258--267 Spatial and temporal requirement of Defective proventriculus activity during Drosophila midgut development. [FBrf0214038] Veenstra, 2009, Cell Tissue Res. 336(2): 309--323 Peptidergic paracrine and endocrine cells in the midgut of the fruit fly maggot. [FBrf0207758] Ciechanska et al., 2007, Genome 50(8): 693--705 dAP-2 and defective proventriculus regulate Serrate and Delta expression in the tarsus of Drosophila melanogaster. [FBrf0201939] Carr et al., 2005, Dev. Genes Evol. 215(8): 402--409 Expression of defective proventriculus during head capsule development is conserved in Drosophila and stalk-eyed flies (Diopsidae). [FBrf0187434] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Kolzer et al., 2003, Development 130(17): 4135--4147 defective proventriculus is required for pattern formation along the proximodistal axis, cell proliferation and formation of veins in the Drosophila wing. [FBrf0160689] Nakagoshi et al., 2002, Dev. Biol. 249(1): 44--56 Refinement of Wingless expression by a Wingless- and Notch-responsive homeodomain protein, defective proventriculus. [FBrf0151715] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Fuss and Hoch, 1998, Mech. Dev. 79(1-2): 83--97 Drosophila endoderm development requires a novel homeobox gene which is a target of Wingless and Dpp signalling. [FBrf0108180] Nakagoshi et al., 1998, Genes Dev. 12(17): 2724--2734 A novel homeobox gene mediates the dpp signal to establish functional specificity within target cells. [FBrf0104547] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] Jackle and Janning, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103143] Vosshall, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103142] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
Abstract	Nakagoshi et al., 1997, A. Dros. Res. Conf. 38: 230B defective proventriculus encodes a homeodomain protein required for midgut development. [FBrf0092263]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{PZ}dve01738 Insertion

Dmel\P{PZ}dve⁰¹⁷³⁸ Insertion