A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{PZ}ena02029 Insertion

General Information
Symbol Dmel\P{PZ}ena02029 Species D. melanogaster
Name FlyBase ID FBti0005205
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) CG15118, Ecol\lacZ, ena Viability / fertility
Causes allele(s) CG1511802029, Ecol\lacZena-02029, ena02029 Stock availability 1 publicly available
LINE ID l(2)02029
Genomic Location
Chromosomal location 2R ( 56B6 ) Sequence location 2R:15,029,836..15,029,843 [+]
Map ( GBrowse )
hide Recent Updates
Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
Update Feed
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
atom feed
FB2012_01
FB2011_10
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:15,029,836..15,029,843 [+]
(Spradling et al., 1999)
2R:15,029,834..15,029,834 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
56B6 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
56C1-56C2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ025592
(FlyBase, 1992-,  
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
CG15118
(FlyBase, 1996-)
Ecol\lacZ
(BDGP Project Members, 1994-1999, Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10)
ena
(BDGP Project Members, 1994-1999, Matusek et al., 2008, Spradling et al., 1999)
hide Alleles and Phenotypes
Causes alleles
CG1511802029
(FlyBase, 1996-)
Ecol\lacZena-02029
(BDGP Project Members, 1994-1999, Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10)
ena02029
(BDGP Project Members, 1994-1999, Gao et al., 1999, FlyBase, 1996-, Spradling et al., 1999, Matusek et al., 2008)
Lethality
References
lethal | recessive
(Spradling et al., 1999)
semi-lethal
 
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
An ena[02029] heterozygous background increases the proportion of DAAM[Ex68] mutant embryos that exhibit CNS defects.
(Matusek et al., 2008)
Stage 15 transheterozygous Df(2R)en-SFX31/ena[02029] embryos exhibit defects in central nervous system scaffolding with gaps in longitudinal tracts, loss of posterior commissures, or fusion of commissures in about 74.5% of the segments.
(Joly et al., 2007)
Heterozygosity for ena02029 does not interfere with the Scer\GAL4P2.4.Pdf>Hsap\APPScer\UAS.T:Hsap\MYC-induced increase in sLNv axonal arborization.
(Leyssen et al., 2005)
S48-5/ena02029 mutants show 7.77%+-2.95 misrotated ommatidia compared to 9.55%+-1.43 seen in S48-5 mutants alone.
(Gaengel and Mlodzik, 2003)
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage  
organism | ubiquitous  
(Jackle and Janning, 1998.8.10)
wandering third instar larval stage  
imaginal disc  
(Meister and Braun, 1995.10)
embryonic/larval brain  
(Meister and Braun, 1995.10)
embryonic/larval midgut  
(Meister and Braun, 1995.10)
Additional Information
Statement
Reference
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)02029
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11114
cn1 P{PZ}ena02029 CG1511802029/CyO; ry506
hide Linkouts
hide Comments
Location 2R:14657192-14657193 confirmed by FlyBase alignment of dbGSS accession AQ025592 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
insertion associated host repeat
(Spradling et al., 1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P1114
(Meister and Braun, 1995.10)
P{PZ}ena02029
(FlyBase, 2005, FlyBase, 1992-, Spradling et al., 1999)
P{PZ}enb02029
(FlyBase, 1992-)
P{PZ}l(2)0202902029
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{ry+t7.2=PZ}l(2)0202902029
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0000284
hide References ( 16 )
Research paper
Matusek et al., 2008, J. Neurosci. 28(49): 13310--13319
Formin proteins of the DAAM subfamily play a role during axon growth. [FBrf0206320]
Joly et al., 2007, Dev. Biol. 301(2): 542--554
Engrailed controls the organization of the ventral nerve cord through frazzled regulation. [FBrf0194180]
Leyssen et al., 2005, EMBO J. 24(16): 2944--2955
Amyloid precursor protein promotes post-developmental neurite arborization in the Drosophila brain. [FBrf0187512]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Gaengel and Mlodzik, 2003, Development 130(22): 5413--5423
Egfr signaling regulates ommatidial rotation and cell motility in the Drosophila eye via MAPK/Pnt signaling and the Ras effector Canoe/AF6. [FBrf0167499]
Gao et al., 1999, Genes Dev. 13(19): 2549--2561
Genes regulating dendritic outgrowth, branching, and routing in Drosophila. [FBrf0111874]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase
Jackle and Janning, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103143]
Vosshall, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103142]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1996-, FlyBase inference based on genome sequence analysis.
FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]