A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{PZ}kermit02045 Insertion

General Information
Symbol Dmel\P{PZ}kermit02045 Species D. melanogaster
Name FlyBase ID FBti0005206
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, kermit Viability / fertility
Causes allele(s) Ecol\lacZkermit-02045, kermit02045 Stock availability 1 publicly available
LINE ID l(2)02045
Genomic Location
Chromosomal location 2R ( 44B5 ) Sequence location 2R:4,039,778..4,039,778 [+]
Map ( GBrowse ) detailed view FBti0132689 FBti0066593 FBti0110188 FBti0125030 FBti0046584 FBti0069195 FBti0109399 FBti0021332 FBti0112313 FBti0055342 FBti0106813 FBti0104751 FBti0011125 FBti0105518 FBti0113004 FBti0057082 FBti0040152 FBti0066747 FBti0143321 FBti0056124 FBti0110911 FBti0026347 FBti0005206 FBti0007609 FBti0105058 FBti0111068 FBti0107164 FBti0057567 FBti0033624 FBti0125031_2 FBti0125031_1 FBti0108198 FBti0111605 FBti0106904 FBti0029699 FBti0077947 FBti0021334 FBti0044645 FBti0047716 FBti0047571 FBti0067447 FBti0053213 FBti0024524
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:4,039,778..4,039,778 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
44B5 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
44B5-44B9 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ025593
(FlyBase, 1992-,  
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999, Vosshall, 1998.8.10)
kermit
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZkermit-02045
(BDGP Project Members, 1994-1999, Vosshall, 1998.8.10)
kermit02045
(BDGP Project Members, 1994-1999, Misra, 2000.8.25, Spradling et al., 1999)
Lethality
References
lethal | recessive
(Spradling et al., 1999)
semi-lethal
 
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
In wandering third instar larva, no β-gal staining observed.
(Meister and Braun, 1995.10)
Marker for
Reflects
expression of
kermit
(Meister and Braun, 1995.10)
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)02045
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11140
cn1 P{PZ}kermit02045/CyO; ry506
hide Linkouts
hide Comments
Location 2R:3664086-3664087 confirmed by FlyBase alignment of dbGSS accession AQ025593 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P1140
(Meister and Braun, 1995.10)
P{PZ}kermit02045
(FlyBase, 1992-)
P{PZ}l(2)0204502045
(FlyBase, 1992-, FlyBase, 2005, BDGP Project Members, 1994-1999)
P{ry+t7.2=PZ}l(2)0204502045
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0000285
hide References ( 10 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase
Misra, 2000.8.25, nc5 report, second installment.
nc5 report, second installment. [FBrf0131112]
Vosshall, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103142]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]