A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{PZ}Nipped-B02047 Insertion

General Information
Symbol Dmel\P{PZ}Nipped-B02047 Species D. melanogaster
Name FlyBase ID FBti0005207
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, Nipped-B Viability / fertility
Causes allele(s) Ecol\lacZNipped-B-02047, Nipped-B02047 Stock availability 1 publicly available
LINE ID l(2)02047
Genomic Location
Chromosomal location 2R ( 41B3-41C1 ) Sequence location
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Description
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location
(computed by FlyBase)
41B3-41C1 ( near gene of known cytology )
Cytological location
(reported)
41C1-41C6 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
h46 (in situ hybridization reported)
(Corradini et al., 2003)
Comments concerning
location
hide Sequence Data
Flanking sequence
G00522
(FlyBase, 1992-,  
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999, Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10)
Nipped-B
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZNipped-B-02047
(BDGP Project Members, 1994-1999, Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10)
Nipped-B02047
(BDGP Project Members, 1994-1999, Corradini et al., 2003, Spradling et al., 1999, Rollins et al., 1999, Jackle and Janning, 1998.8.10)
Lethality
References
lethal
(Coulthard et al., 2010)
lethal | recessive
(Rollins et al., 1999, Corradini et al., 2003, Jackle and Janning, 1998.8.10, Spradling et al., 1999)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
Neuroblast metaphase nuclei show precocious sister chromatid separation in animals heteroallelic for Nipped-B[02047] and Nipped-B[NC6], Nipped-B[NC7], Nipped-B[NC23], Nipped-B[NC39], Nipped-B[NC41], Nipped-B[NC59], Nipped-B[NC71], Nipped-B[NC77] or Nipped-B[NC78].
(Gause et al., 2008)
Approximately 60% of metaphase nuclei in the brains of Nipped-B[407]/Nipped-B[02047] second instar larvae show precocious sister chromatid separation, in which the centromeric regions of the sister chromatids are somewhat detached from each other. 50% of metaphase nuclei in the brains of Nipped-B[292.1]/Nipped-B[02047] and Nipped-B[02047] homozygous second instar larvae show precocious sister chromatid separation, in which the centromeric regions of the sister chromatids are somewhat detached from each other.
(Rollins et al., 2004)
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage  
epidermis  
(Meister and Braun, 1995.10)
embryonic/larval cuticle  
(Meister and Braun, 1995.10)
embryonic/larval midgut  
(Meister and Braun, 1995.10)
embryonic/larval somatic muscle  
(Meister and Braun, 1995.10)
Additional Information
Statement
Reference
Marker for
Reflects
expression of
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)02047
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11143
P{PZ}Nipped-B02047 cn1/CyO; ry506
hide Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P1143
(Meister and Braun, 1995.10)
P{PZ}l(2)02047
(Corradini et al., 2003)
P{PZ}l(2)0204702047
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{PZ}Nipped-B02047
(FlyBase, 1992-)
P{ry+t7.2=PZ}l(2)0204702047
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0000286
hide References ( 14 )
Research paper
Coulthard et al., 2010, Genetics 185(2): 479--495
Essential loci in centromeric heterochromatin of Drosophila melanogaster. I: the right arm of chromosome 2. [FBrf0211235]
Gause et al., 2008, Chromosoma 117(1): 51--66
Functional links between Drosophila Nipped-B and cohesin in somatic and meiotic cells. [FBrf0203143]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Rollins et al., 2004, Molec. Cell. Biol. 24(8): 3100--3111
Drosophila nipped-B protein supports sister chromatid cohesion and opposes the stromalin/Scc3 cohesion factor to facilitate long-range activation of the cut gene. [FBrf0174962]
Corradini et al., 2003, Chromosoma 112(1): 26--37
FISH analysis of Drosophila melanogaster heterochromatin using BACs and P elements. [FBrf0160459]
Rollins et al., 1999, Genetics 152(2): 577--593
Nipped-B, a Drosophila homologue of chromosomal adherins, participates in activation by remote enhancers in the cut and Ultrabithorax genes. [FBrf0109032]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase
Jackle and Janning, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103143]
Vosshall, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103142]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]