Dmel\P{PZ}Rca1⁰³³⁰⁰ Insertion

General Information
Symbol	Dmel\P{PZ}Rca1⁰³³⁰⁰	Species	D. melanogaster
Name		FlyBase ID	FBti0005236
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, Rca1	Viability / fertility
Causes allele(s)	Ecol\lacZ^Rca1-03300, Rca1⁰³³⁰⁰	Stock availability	2 publicly available
LINE ID	l(2)03300
Genomic Location
Chromosomal location	2L ( 27C1 )	Sequence location	2L:6,852,365..6,852,365 [+]
Map ( GBrowse )
Recent Updates
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:6,852,365..6,852,365 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	27C1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	27B4-27C1 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ025609 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999, Vosshall, 1998.8.10, Russell et al., 1998) Rca1 (Dong et al., 1997)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^Rca1-03300 (BDGP Project Members, 1994-1999, Vosshall, 1998.8.10, Russell et al., 1998) Rca1⁰³³⁰⁰ (Dong et al., 1997, BDGP Project Members, 1994-1999, Han and Bodmer, 2003, Spradling et al., 1999)

Lethality References lethal \| recessive (Spradling et al., 1999) semi-lethal
Sterility References
Phenotype Manifest In
	abdominal segment 2 & cardioblast (Han and Bodmer, 2003) abdominal segment 3 & cardioblast (Han and Bodmer, 2003) abdominal segment 4 & cardioblast (Han and Bodmer, 2003) abdominal segment 5 & cardioblast (Han and Bodmer, 2003) abdominal segment 6 & cardioblast (Han and Bodmer, 2003) abdominal segment 7 & cardioblast (Han and Bodmer, 2003) aCC neuron \| precursor (Wai et al., 1999) EL neuron (Lear et al., 1999) embryonic pericardial cell (Han and Bodmer, 2003) ganglion mother cell (Wai et al., 1999) ganglion mother cell GMC1-1a (Lear et al., 1999) ganglion mother cell GMC4-2a (Wai et al., 1999, Lear et al., 1999) pCC neuron (Wai et al., 1999) pCC neuron \| precursor (Wai et al., 1999) RP2sib neuron (Wai et al., 1999) U neuron (Wai et al., 1999)
Detailed Description
Statement Reference 86% of mutant embryos lack eve expressing pericardial cells (EPC), the rest show one EPC per hemisegment (instead of the two seen in wild-type). DA1 muscles form as in wild-type. A halving is also seen in the number of myocardial cells seen in segments A2 to A7, though the number seen in segments T3 to A1 remain normal. (Han and Bodmer, 2003) In mutant embryos, the ganglion mother cells, GMC1-1a and GMC4-2a fail to divide into their normal progeny. The non-dividing GMC1-1a and GMC4-2a cells are larger than normal and sit where its offspring should normally be. A loss of approximately 10% of EL neurons is also seen. (Lear et al., 1999) In mutant embryos the division of neurons are affected in a lineage specific manner. The ganglion mother cell (GMC) of the RP2/sib lineage fails to divide, and assumes the identity of the RP2 neuron. The GMC of the aCC/pCC lineage is similarly affected; it fails to divide and assumes the identity of the aCC. These phenotypes are fully penetrant. Timing and axonal projections from these neurons continue as if they were wildtype. Other lineages are also affected in mutant embryos. The CQ lineage fails to divide in about 50% of hemisegments and the U lineage fails to divide in 65% of hemisegments. The effect on the EL line is minimal. (Wai et al., 1999) Dominantly enhances the rough eye phenotype of CycE^JP homozygotes. One copy of Rca1⁰³³⁰⁰ has no effect on the reduction in the number of cells entering S phase posterior to the morphogenetic furrow seen in CycE^JP homozygotes. (Secombe et al., 1998)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference adult stage optic lobe (Vosshall, 1998.8.10) adult brain \| restricted (Vosshall, 1998.8.10) adult central complex (Vosshall, 1998.8.10)
Additional Information
Statement Reference In the adult brain, expressed in the central brain and the optic lobe. (Vosshall, 1998.8.10) In wandering third instar larva, no detectable β-gal staining. (Meister and Braun, 1995.10)

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)03300 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	11294 P{PZ}Rca1⁰³³⁰⁰ cn¹/CyO; ry⁵⁰⁶
Kyoto	108868 P{PZ}Rca1⁰³³⁰⁰ cn¹/CyO; ry⁵⁰⁶
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{PZ}l(2)03300⁰³³⁰⁰ (FlyBase, 1992-) P{PZ}Rca1⁰³³⁰⁰ (Dong et al., 1997, FlyBase, 1992-) P{ry^+t7.2=PZ}l(2)03300⁰³³⁰⁰ (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000315
References ( 14 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Han and Bodmer, 2003, Development 130(13): 3039--3051 Myogenic cells fates are antagonized by Notch only in asymmetric lineages of the Drosophila heart, with or without cell division. [FBrf0160590] Lear et al., 1999, Mech. Dev. 88(2): 207--219 Neural cell fate in rca1 and cycA mutants: the roles of intrinsic and extrinsic factors in asymmetric division in the Drosophila central nervous system. [FBrf0111940] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Wai et al., 1999, Development 126(12): 2759--2770 Cell division genes promote asymmetric interaction between numb and Notch in the Drosophila CNS. [FBrf0109136] Russell et al., 1998, Genome 41(1): 7--13 Lethal P-lacZ insertion lines expressed during pattern respecification in the imaginal discs of Drosophila. [FBrf0102000] Secombe et al., 1998, Genetics 149(4): 1867--1882 Analysis of a Drosophila cyclin E hypomorphic mutation suggests a novel role for cyclin E in cell proliferation control during eye imaginal disc development. [FBrf0103367] Dong et al., 1997, Genes Dev. 11(1): 94--105 Control of G1 in the developing Drosophila eye: rca1 regulates Cyclin A. [FBrf0091048] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase	Vosshall, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103142] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{PZ}Rca103300 Insertion

Dmel\P{PZ}Rca1⁰³³⁰⁰ Insertion