A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{PZ}Su(var)2-1003697 Insertion

General Information
Symbol Dmel\P{PZ}Su(var)2-1003697 Species D. melanogaster
Name FlyBase ID FBti0005246
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, Su(var)2-10 Viability / fertility
Causes allele(s) Ecol\lacZSu(var)2-10-03697, Su(var)2-1003697 Stock availability 1 publicly available
LINE ID l(2)03697
Genomic Location
Chromosomal location 2R ( 45A8 ) Sequence location 2R:5,004,359..5,004,359 [+]
Map ( GBrowse ) detailed view FBti0051152 FBti0125046_2 FBti0125046_1 FBti0099840 FBti0105963 FBti0023430 FBti0010713 FBti0039662 FBti0021167 FBti0106076 FBti0111167 FBti0027368 FBti0034794 FBti0026152 FBti0026000 FBti0049605 FBti0125047_1 FBti0125047_2 FBti0108242 FBti0108405 FBti0039654 FBti0107557 FBti0110596 FBti0030173 FBti0005246 FBti0099859 FBti0072267 FBti0145536 FBti0112236 FBti0048492 FBti0038094
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Description
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:5,004,359..5,004,359 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
45A8 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
45A4-45A8 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ025614
(FlyBase, 1992-,  
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999, Vosshall, 1998.8.10)
Su(var)2-10
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZSu(var)2-10-03697
(BDGP Project Members, 1994-1999, Vosshall, 1998.8.10)
Su(var)2-1003697
(BDGP Project Members, 1994-1999, Mohr and Boswell, 2002, Spradling et al., 1999, Mohr and Boswell, 1999, Hari et al., 2001)
Lethality
References
lethal | embryonic stage | recessive
(Mohr and Boswell, 1999)
lethal | recessive
(Spradling et al., 1999, Hari et al., 2001)
Sterility
References
hide Phenotype Manifest In
eye | somatic clone
(Betz et al., 2001)
lens | somatic clone
(Betz et al., 2001)
ommatidium | somatic clone
(Betz et al., 2001)
photoreceptor cell | somatic clone
(Betz et al., 2001)
hide Detailed Description
Statement
Reference
The addition of Su(var)2-1003697 to os1 mutants leads to a slight suppression of the small eye phenotype seen in os1 flies. The further addition of Stat92E06346 increases the suppression.
(Betz et al., 2001)
When homozygous mutant clones are made in the eye, the lens structure completely fails to develop and is replaced by a heterogeneous bulged-out surface, lacking bristles. Partially differentiated lenses surround the border of the clone. Cellular differentiation into photoreceptors and other cell types fails, especially inn the centre of the clones. Along the clonal borders partially differentiated ommatidia can be seen, with incomplete sets of photoreceptors. Other clones apparently undergo necrotic and/or apoptotic cell death, because scars in the eyes are frequently found.
(Betz et al., 2001)
Fails to complement Su(var)2-101 and Su(var)2-102 for lethality.
(Hari et al., 2001)
Cuticles of homozygous embryos are indistinguishable from wild-type (97%) or occasionally have minor defects (3%).
(Mohr and Boswell, 1999)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
In wandering third instar larva, no detectable β-gal staining.
(Meister and Braun, 1995.10)
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)03697
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11344
cn1 P{PZ}Su(var)2-1003697/CyO; ry506
hide Linkouts
hide Comments
Location 2R:4628667-4628668 confirmed by FlyBase alignment of dbGSS accession AQ025614 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P1344
(Meister and Braun, 1995.10)
P{PZ}l(2)0369703697
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{PZ}Su(var)2-1003697
(FlyBase, 2005, FlyBase, 1992-)
P{PZ}zimp03697
(FlyBase, 1992-)
P{ry+t7.2=PZ}l(2)0369703697
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0000329
hide References ( 13 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Mohr and Boswell, 2002, Genetics 160(4): 1503--1510
Genetic analysis of Drosophila melanogaster polytene chromosome region 44D-45F. Loci required for viability and fertility. [FBrf0147061]
Betz et al., 2001, Proc. Natl. Acad. Sci. USA 98(17): 9563--9568
A Drosophila PIAS homologue negatively regulates stat92E. [FBrf0137351]
Hari et al., 2001, Genes Dev. 15(11): 1334--1348
The Drosophila Su(var)2-10 locus regulates chromosome structure and function and encodes a member of the PIAS protein family. [FBrf0136940]
Mohr and Boswell, 1999, Gene 229(1-2): 109--116
Zimp encodes a homologue of mouse Miz1 and PIAS3 and is an essential gene in Drosophila melanogaster. [FBrf0108240]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase
Vosshall, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103142]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]