A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{PZ}Cam03909 Insertion

General Information
Symbol Dmel\P{PZ}Cam03909 Species D. melanogaster
Name FlyBase ID FBti0005254
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) Cam, Ecol\lacZ Viability / fertility
Causes allele(s) Cam03909, Ecol\lacZCam-03909 Stock availability 1 publicly available
LINE ID l(2)03909
Genomic Location
Chromosomal location 2R ( 48F1 ) Sequence location 2R:8,146,856..8,146,856 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0051821 FBti0055754 FBti0024737 FBti0108569 FBti0056881 FBti0033503 FBti0051340 FBti0070279 FBti0101467 FBti0146104 FBti0105409 FBti0011308 FBti0005254 FBti0054422 FBti0039984 FBti0108491 FBti0072667 FBti0077048 FBti0007755 FBti0044849 FBti0044846 FBti0048596 FBti0143662 FBti0024572 FBti0010858 FBti0109514 FBti0010996 FBti0007725 FBti0059078 FBti0026178 FBti0077047 FBti0111542 FBti0054660 FBti0028110 FBti0007017 FBti0068907 FBti0130043 FBti0077046 FBti0107505 FBti0033517 FBti0099780 FBti0112249 FBti0006907 FBti0036060 FBti0029143 FBti0143630 FBti0044346 FBti0125129 FBti0069071
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Spradling et al., 1999, Bellen et al., 2004)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:8,146,856..8,146,856 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
48F1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
48F1--48F2 (in situ hybridization reported)
(Bogart and Cook, 2000.11.8)
48F1-48F2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AF006643
(FlyBase, 1992-)
AQ073279
(FlyBase, 1992-,  
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Cam
(BDGP Project Members, 1994-1999)
Ecol\lacZ
(BDGP Project Members, 1994-1999, Harvie et al., 1998, Vosshall, 1998.8.10)
hide Alleles and Phenotypes
Causes alleles
Cam03909
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Harvie et al., 1998)
Ecol\lacZCam-03909
(BDGP Project Members, 1994-1999, Harvie et al., 1998, Vosshall, 1998.8.10)
Lethality
References
lethal | recessive
(Spradling et al., 1999, Harvie et al., 1998)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
Heterozygosity for Cam[03909] partially rescues the flightless phenotype caused by expression of either Hsap\MYBPC3[Scer\UAS.T:Hsap\MYC], Hsap\MYBPC3[M6.Scer\UAS.T:Hsap\MYC] or Hsap\MYBPC3[M0.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[sd-SG29.1].
(Manh et al., 2005)
The amount of backward movement seen in Cam03909/Camn339 larvae is increased by high light intensity.
(Wang et al., 2003)
Shows an extended third instar phenotype.
(Harvie et al., 1998)
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage  
embryonic/larval corpus allatum  
(Harvie et al., 1998)
prothoracic gland  
(Harvie et al., 1998)
wandering third instar larval stage  
imaginal disc  
(Harvie et al., 1998)
embryonic/larval optic lobe  
(Harvie et al., 1998)
ventral nerve cord  
(Harvie et al., 1998)
adult stage  
adult brain | restricted  
(Vosshall, 1998.8.10)
adult central complex | restricted  
(Vosshall, 1998.8.10)
Additional Information
Statement
Reference
During all larval stages, expressed in lateral secretory cells (prothoracic gland) and medial secretory cells (corpus allatum) of the ring gland.
(Harvie et al., 1998)
In the adult, expressed in a discrete region of the central brain.
(Vosshall, 1998.8.10)
Marker for
Reflects
expression of
Cam
(Harvie et al., 1998)
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)03909
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11356
w1118; cn1 P{PZ}Cam03909/CyO, P{sevRas1.V12}FK1
hide Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{PZ}Cam03909
(BDGP Project Members, 1994-1999, FlyBase, 1992-, )
P{PZ}l(2)0390903909
(FlyBase, 1992-)
P{ry+t7.2=PZ}l(2)0390903909
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0000338
hide References ( 13 )
Research paper
Manh et al., 2005, Hum. Mol. Genet. 14(1): 7--17
Expression of cardiac myosin-binding protein-C (cMyBP-C) in Drosophila as a model for the study of human cardiomyopathies. [FBrf0187722]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Wang et al., 2003, Genetics 165(3): 1255--1268
Drosophila calmodulin mutants with specific defects in the musculature or in the nervous system. [FBrf0167631]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Harvie et al., 1998, Genetics 149(1): 217--231
Genes expressed in the ring gland, the major endocrine organ of Drosophila melanogaster. [FBrf0102844]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Supplementary material
Parks et al., 2004, Nature Genetics 36(3):
Supplementary Table 1: Deletions generated at Bloomington between trans-heterozygous P element insertions. [FBrf0179070]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
Bogart and Cook, 2000.11.8, Isolation of Df(2R)BSC3.
Isolation of Df(2R)BSC3. [FBrf0132168]
Vosshall, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103142]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]