A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{PZ}crol04418 Insertion

General Information
Symbol Dmel\P{PZ}crol04418 Species D. melanogaster
Name FlyBase ID FBti0005267
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) crol, Ecol\lacZ Viability / fertility
Causes allele(s) crol04418, Ecol\lacZcrol-04418 Stock availability 1 publicly available
LINE ID l(2)04418
Genomic Location
Chromosomal location 2L ( 33A1 ) Sequence location 2L:11,803,899..11,806,047 [-]
Map ( GBrowse )
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Description
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This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:11,803,899..11,806,047 [-]
(D'Avino and Thummel, 1998)
2L:11,805,385..11,805,385 [+]
Orientation
Cytological location
(computed by FlyBase)
33A1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
33A1-33A2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
G00541
(FlyBase, 1992-,  
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
crol
(BDGP Project Members, 1994-1999, D'Avino and Thummel, 1998)
Ecol\lacZ
(BDGP Project Members, 1994-1999, Vosshall, 1998.8.10, Jackle and Janning, 1998.8.10, D'Avino and Thummel, 1998)
hide Alleles and Phenotypes
Causes alleles
crol04418
(BDGP Project Members, 1994-1999, D'Avino and Thummel, 2000, D'Avino and Thummel, 1998, Spradling et al., 1999)
Ecol\lacZcrol-04418
(BDGP Project Members, 1994-1999, Vosshall, 1998.8.10, Jackle and Janning, 1998.8.10, D'Avino and Thummel, 1998)
Lethality
References
lethal | pupal stage | recessive
(D'Avino and Thummel, 1998)
lethal | recessive
(Spradling et al., 1999)
Sterility
References
hide Phenotype Manifest In
adult head
(D'Avino and Thummel, 1998)
leg
(D'Avino and Thummel, 1998)
metathoracic femur
(D'Avino and Thummel, 1998)
metathoracic leg
(D'Avino and Thummel, 1998)
metathoracic leg sensillum
(D'Avino and Thummel, 1998)
pupa
(D'Avino and Thummel, 1998)
stage P5 pupa
(D'Avino and Thummel, 1998)
stage P14 pharate adult
(D'Avino and Thummel, 1998)
ventral thoracic disc
(D'Avino and Thummel, 1998)
hide Detailed Description
Statement
Reference
The addition of crol04418 enhances the wing blistering phenotype of if3/Y flies by 3-4 fold. The addition of crol04418 does not enhance the wing blistering phenotype of mys8/Y flies.
(D'Avino and Thummel, 2000)
Homozygotes survive through embryogenesis and hatch to form first instar larvae. Homozygous larvae progress normally through prepupal development, showing no delay in puparium formation, but die before adult eclosion. Homozygotes die during two stages of pupal development. The first lethal phase occurs at the beginning of pupal development, during stage P5. Pupae that arrest at this stage have severe defects in head eversion and leg elongation. Approximately 40-50% of homozygotes die at stage P14, at the end of pupal development, with a microcephalic phenotype and malformed legs. The penetrance of the microcephalic phenotype is variable, while the "crooked legs" phenotype is highly penetrant and similar in all legs examined. The third pair of legs are smaller than wild-type, have a kink near the middle of the femur and lack some bristles in hemizygotes. Hemizygous leg discs show a range of defects from partial elongation to severe malformation. Legs showing partial elongation also have slightly more expanded 2nd to 5th tarsal segments compared to wild-type legs. Imaginal discs are normal in size and morphology.
(D'Avino and Thummel, 1998)
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage  
organism | segmentally repeated  
(Jackle and Janning, 1998.8.10)
embryonic mandibular segment  
(Jackle and Janning, 1998.8.10)
presumptive embryonic/larval central nervous system  
(Jackle and Janning, 1998.8.10)
embryonic/larval brain | presumptive  
(Jackle and Janning, 1998.8.10)
organism | anterior  
(Jackle and Janning, 1998.8.10)
presumptive embryonic/larval peripheral nervous system | segmentally repeated  
(Jackle and Janning, 1998.8.10)
third instar larval stage  
embryonic/larval corpus allatum  
(D'Avino and Thummel, 1998)
embryonic/larval optic lobe  
(D'Avino and Thummel, 1998)
ventral thoracic disc  
(D'Avino and Thummel, 1998)
ventral nerve cord  
(D'Avino and Thummel, 1998)
tarsal segment | precursor  
(D'Avino and Thummel, 1998)
prepupal stage  
embryonic/larval corpus allatum  
(D'Avino and Thummel, 1998)
embryonic/larval fat body  
(D'Avino and Thummel, 1998)
trachea  
(D'Avino and Thummel, 1998)
femur | precursor  
(D'Avino and Thummel, 1998)
tibia | precursor  
(D'Avino and Thummel, 1998)
prepupal stage P1  
embryonic/larval salivary gland  
(D'Avino and Thummel, 1998)
Additional Information
Statement
Reference
Reporter gene expression is observed in leg imaginal discs isolated from late third instar larvae. Expression is initially restricted to the precursors of the tarsal segments. In prepupal leg discs, the expression expands to the precursors of the tibia and the femur. Expression is induced in the salivary glands at puparium formation. Expression is also observed in the fat body and in the trachea. Expression is observed in the ventral ganglion and in the presumptive optic lobes of the CNS in mid-third instar larvae and increases noticably at puparium formation. Finally, expression is observed in the corpus allatum of the ring gland.
(D'Avino and Thummel, 1998)
In early embryo, expression in an 'anterior ring of cells'; expression in mandibular segment not certain.
(Jackle and Janning, 1998.8.10)
In wandering third instar larva, no detectable β-gal staining.
(Meister and Braun, 1995.10)
Marker for
Reflects
expression of
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)04418
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11374
P{PZ}crol04418 cn1/CyO; ry506
hide Linkouts
hide Comments
insertion mapped to second intron
(D'Avino and Thummel, 1998)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P1374
(Meister and Braun, 1995.10)
P{PZ}crol04418
(FlyBase, 1992-, D'Avino and Thummel, 1998)
P{PZ}l(2)0441804418
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{ry+t7.2=PZ}l(2)0441804418
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0000352
hide References ( 11 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
D'Avino and Thummel, 2000, Dev. Biol. 220(2): 211--224
The ecdysone regulatory pathway controls wing morphogenesis and integrin expression during Drosophila metamorphosis. [FBrf0127048]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
D'Avino and Thummel, 1998, Development 125(9): 1733--1745
crooked legs encodes a family of zinc finger proteins required for leg morphogenesis and ecdysone-regulated gene expression during Drosophila metamorphosis. [FBrf0102633]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase
Jackle and Janning, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103143]
Vosshall, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103142]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]