FlyBase Insertion Report: Dmel\P{PZ}smt3[04493]

General Information

Symbol

Dmel\P{PZ}smt3⁰⁴⁴⁹³

Species

D. melanogaster

Name

FlyBase ID

FBti0005271

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

Uses

lacZ reporter

Affected gene(s)

Ecol\lacZ, smt3

Viability / fertility

Associated allele(s)

Ecol\lacZ^smt3-04493, smt3⁰⁴⁴⁹³

Stock availability

1 publicly available

LINE ID

l(2)04493

Genomic Location

Chromosomal Location

2L ( 27C7 )

Sequence Location

2L:6,967,613..6,967,613 [+]

Target / Docking site

Genomic Maps

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Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:6,967,613..6,967,613 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

27C7 (inferred by FlyBase from sequence location)

Cytological location (reported)

27C7-27C8 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Genomic location previously reported as 2L:6967604..6967612 .

Sequence Data

Flanking sequence

AQ025627

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Location-dependent role

lacZ enhancer trap

(Mlodzik and Hiromi, 1992)

Size (Kb)

14.545

Associated alleles

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

smt3

(Saja et al., 2010, Sánchez et al., 2010, Chen et al., 2008, BDGP Project Members, 1994-1999)

Alleles and Phenotypes

Allele

Ecol\lacZ^smt3-04493

Reference(s)

(Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

Phenotype Data

Phenotypic Class Phenotype Manifest in Detailed Description

Allele

smt3⁰⁴⁴⁹³

Reference(s)

(Saja et al., 2010, Sánchez et al., 2010, Chen et al., 2008, Schnorr et al., 2001, Misra, 2000.8.25, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Phenotype Data

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval brain | presumptive