FB2025_01 , released February 20, 2025
Insertion: Dmel\P{PZ}Eb104524
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General Information
Symbol
Dmel\P{PZ}Eb104524
Species
D. melanogaster
Name
FlyBase ID
FBti0005272
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{PZ}
LINE ID

l(2)04524

Affected gene(s)
Eb1, Ecol\lacZ, Mccc2
Viability / fertility
Associated allele(s)
Eb104524, Ecol\lacZEb1-04524, Mccc204524
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
2R ( 42C8 )
Sequence Location
2R:6,749,376..6,749,376 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{PZ}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:6,749,376..6,749,376 [+]
(FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)
42C8 (inferred by FlyBase from sequence location)
Cytological location (reported)
42F1-42F2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Inserted Element
Construct
P{PZ}
Size (Kb)

14.545

Component Allele(s)
l(3)87Df7.2
ry+t7.2
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
ring gland
(Harvie et al., 1998)
wandering third instar larval stage
imaginal disc
(Meister and Braun, 1995.10)
embryonic/larval optic lobe
(Harvie et al., 1998)
larval ventral nerve cord
(Harvie et al., 1998)
Additional Information
Statement
Reference

Expressed in ring gland during all larval stagess; expression observed in lateral secretory cells (prothoracic gland), medial secretory cells (corpus allatum) and corpus cardiacum cells.

(Harvie et al., 1998)

In wandering third instar larva, strong expression in imaginal discs (which discs not specified).

(Meister and Braun, 1995.10)
 
Marker for
Reflects expression of
Eb1
(Harvie et al., 1998)
Reporter construct used in assay
Data on Genetic Line
Line ID

l(2)04524

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Location 2R:2261188-2261189 determined by FlyBase alignment of dbGSS accession AQ025628 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
4524
(Harvie et al., 1998)
P{PZ}Eb104524
(FlyBase, 2005, FlyBase, 1992-)
P{PZ}l(2)0452404524
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{ry+t7.2=PZ}l(2)0452404524
(FlyBase, 1992-)
eb104524
(Bulgakova et al., 2013)
l(2)04524
(Elliott et al., 2005)
Name Synonyms
Secondary FlyBase IDs
  • FBti0000357
References (18)