FlyBase Insertion Report: Dmel\P{PZ}Cka[05836]

General Information

Symbol

Dmel\P{PZ}Cka⁰⁵⁸³⁶

Species

D. melanogaster

Name

FlyBase ID

FBti0005298

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)05836

Affected gene(s)

Cka, Ecol\lacZ

Viability / fertility

Associated allele(s)

Cka⁰⁵⁸³⁶, Ecol\lacZ^Cka-05836

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2L ( 28D3 )

Sequence Location

2L:8,031,104..8,031,104 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:8,031,104..8,031,104 [+]

(FlyBase, 2005)

Orientation

Cytological location (computed by FlyBase)

28D3 (inferred by FlyBase from sequence location)

Cytological location (reported)

28E1-28E2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ025643

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Cka

(Liaw and Chiang, 2019, BDGP Project Members, 1994-1999)

Ecol\lacZ

(Harvie et al., 1998, Jackle and Janning, 1998.8.10, Russell et al., 1998, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Cka⁰⁵⁸³⁶

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ^Cka-05836

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval hindgut

(Meister and Braun, 1995.10)

embryonic/larval optic lobe

(Harvie et al., 1998)

larval ventral nerve cord

(Harvie et al., 1998)

imaginal disc

(Meister and Braun, 1995.10)

gonad

(Meister and Braun, 1995.10)

ring gland

(Harvie et al., 1998)

Malpighian tubule

(Meister and Braun, 1995.10)

adult stage

adult central complex | restricted

(Vosshall, 1998.8.10)

adult brain | restricted

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In the adult, expressed in a discrete region of the central brain.

(Vosshall, 1998.8.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)05836

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11451

P{PZ}Cka⁰⁵⁸³⁶ cn¹/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Comments

Location 2L:8031103-8031104 determined by FlyBase alignment of dbGSS accession AQ025643 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

P{PZ}Cka⁰⁵⁸³⁶

(FlyBase, 2005, FlyBase, 1992-)

P{PZ}WD-40-family-member⁰⁵⁸³⁶

(FlyBase, 1992-)

P{PZ}l(2)05836⁰⁵⁸³⁶

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)05836⁰⁵⁸³⁶

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000384

References (17)

Report Sections

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