Dmel\P{PZ}Hmgs⁰⁶²¹⁴ Insertion

General Information
Symbol	Dmel\P{PZ}Hmgs⁰⁶²¹⁴	Species	D. melanogaster
Name		FlyBase ID	FBti0005301
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, Hmgs	Viability / fertility
Causes allele(s)	Ecol\lacZ^Hmgs-06214, Hmgs⁰⁶²¹⁴	Stock availability	1 publicly available
LINE ID	l(2)06214
Genomic Location
Chromosomal location	2R ( 53C1 )	Sequence location	2R:12,224,283..12,224,283 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:12,224,283..12,224,283 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	53C1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	53C1-53C2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ025645 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999, Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10) Hmgs (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^Hmgs-06214 (BDGP Project Members, 1994-1999, Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10) Hmgs⁰⁶²¹⁴ (BDGP Project Members, 1994-1999, Spradling et al., 1999)

Lethality References lethal \| embryonic stage \| maternal effect \| poor (Perrimon et al., 1996) lethal \| pupal stage \| recessive (Perrimon et al., 1996) lethal \| recessive (Spradling et al., 1999)
Sterility References
Phenotype Manifest In
	abdomen \| dorsal \| maternal effect \| posterior (Perrimon et al., 1996) embryonic/first instar larval cuticle \| maternal effect (Perrimon et al., 1996)
Detailed Description
Statement Reference Germline clones produce eggs with patterning defects: tail on dorsal side. (Perrimon et al., 1996)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage anterior embryonic/larval midgut (Jackle and Janning, 1998.8.10) organism \| segmentally repeated (Jackle and Janning, 1998.8.10) presumptive embryonic/larval peripheral nervous system (Jackle and Janning, 1998.8.10)
Additional Information
Statement Reference In embryo, expression in PNS uncertain. (Jackle and Janning, 1998.8.10) In wandering third instar larva, no detectable β-gal staining. (Meister and Braun, 1995.10)

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)06214 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	11469 cn¹ P{PZ}Hmgs⁰⁶²¹⁴/CyO; ry⁵⁰⁶
Linkouts
Comments
	Location 2R:11851641-11851642 confirmed by FlyBase alignment of dbGSS accession AQ025645 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P1469 (Meister and Braun, 1995.10) P{PZ}Hmgs⁰⁶²¹⁴ (FlyBase, 2005, FlyBase, 1992-) P{PZ}l(2)06214⁰⁶²¹⁴ (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{ry^+t7.2=PZ}l(2)06214⁰⁶²¹⁴ (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000386
References ( 11 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Perrimon et al., 1996, Genetics 144(4): 1681--1692 Zygotic lethal mutations with maternal effect phenotypes in Drosophila melanogaster. [FBrf0091142] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase	Jackle and Janning, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103143] Vosshall, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103142] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{PZ}Hmgs06214 Insertion

Dmel\P{PZ}Hmgs⁰⁶²¹⁴ Insertion