A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{PZ}Sec61β07214 Insertion

General Information
Symbol Dmel\P{PZ}Sec61β07214 Species D. melanogaster
Name FlyBase ID FBti0005327
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, Sec61β Viability / fertility
Causes allele(s) Ecol\lacZSec61β-07214, Sec61β07214 Stock availability 1 publicly available
LINE ID l(2)07214
Genomic Location
Chromosomal location 2R ( 51B6 ) Sequence location
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Description
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location
(computed by FlyBase)
51B6 ( near gene of known cytology )
Cytological location
(reported)
51B7-51B10 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
G00586
(FlyBase, 1992-,  
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(Meister and Braun, 1995.10, BDGP Project Members, 1994-1999, Vosshall, 1998.8.10, Russell et al., 1998, McClure and Schubiger, 2008)
Sec61β
(BDGP Project Members, 1994-1999, McClure and Schubiger, 2008)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZSec61β-07214
(Meister and Braun, 1995.10, BDGP Project Members, 1994-1999, Vosshall, 1998.8.10, Russell et al., 1998, McClure and Schubiger, 2008)
Sec61β07214
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Valcarcel et al., 1999, McClure and Schubiger, 2008)
Lethality
References
lethal | embryonic stage | partially | rescuable maternal effect
(Perrimon et al., 1996)
lethal | pupal stage | recessive
(Perrimon et al., 1996)
lethal | recessive
(Spradling et al., 1999, Valcarcel et al., 1999)
Sterility
References
hide Phenotype Manifest In
abdomen | germline clone | posterior
(Perrimon et al., 1996)
embryonic/first instar larval cuticle
(Valcarcel et al., 1999)
embryonic/first instar larval cuticle | germline clone
(Valcarcel et al., 1999, Perrimon et al., 1996)
head | germline clone
(Perrimon et al., 1996)
leg
(Valcarcel et al., 1999)
hide Detailed Description
Statement
Reference
The frequency of leg-to-wing transdetermination caused by expression of wg[Act5C.PS] in clones during the mid-second larval instar is dominantly suppressed by Sec61β[07214].
(McClure and Schubiger, 2008)
Homozygous embryos have similar cuticle defects to Sec61β14448 homozygotes. Embryos derived from homozygous germ-line clones show poor cuticle development. Sec61β07214/Sec61βk07836 escapers show defects in leg development.
(Valcarcel et al., 1999)
Lethality is rescued by Sec61βTub.PV.
(Valcarcel et al., 1999)
Germline clones produce eggs with poor cuticle development. Paternally rescued eggs exhibit head and tail defects.
(Perrimon et al., 1996)
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage  
embryonic/larval brain  
(Meister and Braun, 1995.10)
Additional Information
Statement
Reference
In wandering third instar, weak β-gal staining in the larval brain.
(Meister and Braun, 1995.10)
Marker for
Reflects
expression of
Sec61β
(Meister and Braun, 1995.10)
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)07214
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
12339
P{PZ}Sec61β07214 cn1/CyO; ry506
hide Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{PZ}l(2)0721407214
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{PZ}Sec61β07214
(FlyBase, 1992-)
P{ry+t7.2=PZ}l(2)0721407214
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0000413
hide References ( 13 )
Research paper
McClure and Schubiger, 2008, Mech. Dev. 125(1-2): 67--80
A screen for genes that function in leg disc regeneration in Drosophila melanogaster. [FBrf0203146]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Shaffer et al., 2002, Proc. Natl. Acad. Sci. USA 99(22): 14332--14337
Heterochromatin protein 2 (HP2), a partner of HP1 in Drosophila heterochromatin. [FBrf0152321]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Valcarcel et al., 1999, J. Cell Sci. 112(23): 4389--4396
Sec61(), a subunit of the protein translocation channel, is required during Drosophila development. [FBrf0112063]
Russell et al., 1998, Genome 41(1): 7--13
Lethal P-lacZ insertion lines expressed during pattern respecification in the imaginal discs of Drosophila. [FBrf0102000]
Perrimon et al., 1996, Genetics 144(4): 1681--1692
Zygotic lethal mutations with maternal effect phenotypes in Drosophila melanogaster. [FBrf0091142]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase
Vosshall, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103142]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]