Dmel\P{PZ}lid¹⁰⁴²⁴ Insertion

General Information
Symbol	Dmel\P{PZ}lid¹⁰⁴²⁴	Species	D. melanogaster
Name		FlyBase ID	FBti0005346
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, lid	Viability / fertility
Causes allele(s)	Ecol\lacZ^lid-10424, lid¹⁰⁴²⁴	Stock availability	1 publicly available
LINE ID	l(2)10424
Genomic Location
Chromosomal location	2L ( 26B2 )	Sequence location	2L:5,998,038..5,998,038 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{PZ}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue. Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:5,998,038..5,998,038 [+]
Orientation
Cytological location (computed by FlyBase)	26B2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	26A8-26A9 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	G01406 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Meister and Braun, 1995.10, BDGP Project Members, 1994-1999, Vosshall, 1998.8.10, Jackle and Janning, 1998.8.10) lid (BDGP Project Members, 1994-1999, DiTacchio et al., 2011)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^lid-10424 (Meister and Braun, 1995.10, BDGP Project Members, 1994-1999, Vosshall, 1998.8.10, Jackle and Janning, 1998.8.10) lid¹⁰⁴²⁴ (BDGP Project Members, 1994-1999, Spradling et al., 1999, Gildea et al., 2000, DiTacchio et al., 2011)

Lethality References lethal (Gildea et al., 2000) lethal \| recessive (Spradling et al., 1999, Gildea et al., 2000, Li et al., 2010) partially lethal - majority die (Secombe et al., 2007, )
Sterility References
Phenotype Manifest In
	embryonic/larval optic lobe (Gildea et al., 2000) imaginal disc (Gildea et al., 2000)
Detailed Description
Statement Reference lid[10424]/+ enhances the variegation at the w locus which is seen in the In(1)w[m4h] chromosome and enhances the variegation at the mutant Sb locus which is seen in the T(2;3)Sb[V] chromosome. (Di Stefano et al., 2011) lid[10424]/+ partially suppresses the held-out wing phenotype and largely suppresses the extra wing vein phenotype which is seen in Su(var)3-3[ΔN] homozygotes. lid[10424]/+ partially suppresses the follicle cell defects seen in Su(var)3-3[ΔN] females. Su(var)3-9[1]/+ suppresses the ability of lid[10424]/+ to enhance the variegation at the w locus which is seen in the In(1)w[m4] chromosome. The wing vein truncation phenotype which is seen in N[Ax-16]/+ flies is enhanced by lid[10424]/+. (Di Stefano et al., 2011) Expression of lid[+mDa] rescues the locomotor rhythm defects seen in homozygous lid[10424] mutant flies. Expression of lid[gJmjC.mut] partially rescues the locomotor rhythm defects seen in homozygous lid[10424] mutant flies. Under LD (12 hours of light:12 hours of dark) and DD conditions (constant darkness) flies exhibit attenuated day:night differences in activity, with flies showing increased activity around midday and reduced dark anticipation. (DiTacchio et al., 2011) Heterozygous lid[10424] mutants show normal locomotor activity under a 12 hours of light:12 hours of dark (LD) cycle and total activity is equivalent to that of wild type. Under constant darkness (DD) lid[10424]/+ flies exhibit disrupted circadian activity rhythms, with the majority of flies showing either no or weak circadian activity rhythms. In flies that do show detectable rhythms, the period is significantly shortened. (DiTacchio et al., 2011) The small, rough eye phenotype caused by expression of asf1[Scer\UAS.cMa] under the control of Scer\GAL4[ey.PH] is significantly suppressed by lid[10424]/+. (Moshkin et al., 2009) Heterozygous flies show strong enhancement of position effect variegation of the w gene caused by In(1)w[m4h]. (Lloret-Llinares et al., 2008) lid^k06801/lid¹⁰⁴²⁴ animals eclose at approximately half the expected frequency. (Secombe et al., 2007) The dm^P0 ; lid^k06801/lid¹⁰⁴²⁴ combination results in almost complete lethality. (Secombe et al., 2007) lid¹⁰⁴²⁴ ash1²² double heterozygotes show T3 to T2 transformations. (Gildea et al., 2000) Some homozygotes and lid^k06801/lid¹⁰⁴²⁴ flies appear to die before hatching, although no obvious defects in the larval cuticle are seen. Most homozygotes appear to die at an early pupal stage. Third instar homozygotes often have a small optic brain lobe and small imaginal discs. (Gildea et al., 2000)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage organism \| anterior (Jackle and Janning, 1998.8.10) embryonic/larval brain \| presumptive (Jackle and Janning, 1998.8.10) presumptive embryonic/larval peripheral nervous system \| segmentally repeated (Jackle and Janning, 1998.8.10) presumptive embryonic/larval central nervous system (Jackle and Janning, 1998.8.10) hypopharyngeal lobe (Jackle and Janning, 1998.8.10) cephalic furrow \| anterior to (Jackle and Janning, 1998.8.10) wandering third instar larval stage embryonic/larval brain (Meister and Braun, 1995.10)
Additional Information
Statement Reference In embryo, expression in hypopharyngeal lobe not certain. (Jackle and Janning, 1998.8.10) In wandering third instar, weak β-gal staining in the larval brain. (Meister and Braun, 1995.10)

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)10424 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	12367 cn¹ P{PZ}lid¹⁰⁴²⁴/CyO; ry⁵⁰⁶
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P2367 (Meister and Braun, 1995.10) P{PZ}l(2)10424¹⁰⁴²⁴ (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{PZ}lid¹⁰⁴²⁴ (FlyBase, 1992-, ) P{ry^+t7.2=PZ}l(2)10424¹⁰⁴²⁴ (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000432
References ( 17 )
Research paper	Di Stefano et al., 2011, Genes Dev. 25(1): 17--28 Functional antagonism between histone H3K4 demethylases in vivo. [FBrf0212709] DiTacchio et al., 2011, Science 333(6051): 1881--1885 Histone lysine demethylase JARID1a activates CLOCK-BMAL1 and influences the circadian clock. [FBrf0216307] Li et al., 2010, PLoS Genet. 6(11): e1001221 Essential functions of the histone demethylase lid. [FBrf0212448] Moshkin et al., 2009, Mol. Cell 35(6): 782--793 Histone chaperones ASF1 and NAP1 differentially modulate removal of active histone marks by LID-RPD3 complexes during NOTCH silencing. [FBrf0209055] Lloret-Llinares et al., 2008, Nucleic Acids Res. 36(9): 2852--2863 Characterization of Drosophila melanogaster JmjC+N histone demethylases. [FBrf0205147] Secombe et al., 2007, Genes Dev. 21(5): 537--551 The Trithorax group protein Lid is a trimethyl histone H3K4 demethylase required for dMyc-induced cell growth. [FBrf0193047] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Gildea et al., 2000, Genetics 156(2): 645--663 A screen for new trithorax group genes identified little imaginal discs, the Drosophila melanogaster homologue of human retinoblastoma binding protein 2. [FBrf0129826] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] Jackle and Janning, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103143] Vosshall, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103142] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{PZ}lid10424 Insertion

Dmel\P{PZ}lid¹⁰⁴²⁴ Insertion