Dmel\P{PZ}Sdc¹⁰⁶⁰⁸ Insertion

General Information
Symbol	Dmel\P{PZ}Sdc¹⁰⁶⁰⁸	Species	D. melanogaster
Name		FlyBase ID	FBti0005354
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, Sdc	Viability / fertility
Causes allele(s)	Ecol\lacZ^Sdc-10608, Sdc¹⁰⁶⁰⁸	Stock availability	none publicly available
LINE ID	l(2)10608
Genomic Location
Chromosomal location	2R ( 57E5 )	Sequence location	2R:17,364,231..17,364,231 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{PZ}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue. Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02	Controlled Vocabulary Terms larval stage NMJ bouton
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:17,364,231..17,364,231 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	57E5 ( inferred by FlyBase from sequence location )
Cytological location (reported)	57E1-57E2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ034166 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999, Vosshall, 1998.8.10) Sdc (BDGP Project Members, 1994-1999, Ren et al., 2009)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^Sdc-10608 (BDGP Project Members, 1994-1999, Vosshall, 1998.8.10) Sdc¹⁰⁶⁰⁸ (Rawson, 2005, BDGP Project Members, 1994-1999, Rawson et al., 2005, Fox and Zinn, 2005, Fox and Zinn, 2005, Lincecum et al., 2000, Spradling et al., 1999, Ren et al., 2009)

Lethality References lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Sterility References
Phenotype Manifest In
	eye photoreceptor cell & axon (Rawson et al., 2005) lamina plexus (Rawson et al., 2005) medulla (Rawson et al., 2005) NMJ bouton \| larval stage (Johnson et al., 2006) presumptive embryonic/larval central nervous system (Johnson et al., 2004)
Detailed Description
Statement Reference Expression of Sdc[Scer\UAS.cJa] under the control of Scer\GAL4[elav.PU] almost completely rescues the reduction in bouton number at the neuromuscular junction which is seen in Sdc[10608]/Df(2R)48 larvae (carrying Sara[Ubi.PJ] to provide Sara function). Expression of Sdc[Scer\UAS.cJa] under the control of Scer\GAL4[how-24B] provides limited rescue of the reduction in bouton number at the neuromuscular junction which is seen in homozygous Sdc[10608] larvae. (Johnson et al., 2006) Homozygous larvae show a significant reduction in bouton number at the neuromuscular junction compared to wild type. Sdc[10608]/Df(2R)48 larvae (carrying Sara[Ubi.PJ] to provide Sara function) show a significant reduction in bouton number at the neuromuscular junction compared to wild type. Mean active zone area and number of active zones per bouton are normal. The amplitude of the evoked excitatory junctional potential (EJP) is normal at the neuromuscular junction of Sdc[10608]/Df(2R)48 larvae (carrying Sara[Ubi.PJ] to provide Sara function). The amplitude and frequency of miniature EJPs are not significantly different from normal. (Johnson et al., 2006) Expression of dlp[Scer\UAS.cBa] under the control of Scer\GAL4[how-24B] does not rescue the reduction in bouton number at the neuromuscular junction seen in homozygous Sdc[10608] larvae. (Johnson et al., 2006) Sdc¹⁰⁶⁰⁸ increases the penetrance of the Lar^bypass ISNb bypass phenotype by more than 2-fold. Sdc⁴⁸/Sdc¹⁰⁶⁰⁸; Lar^13.2/Lar^5.5 (in which Sara has been rescued by expression of the Sara^Ubi.PJ transgene) mutants display an increased penetrance of the bypass phenotype (43% vs. 28%) relative to the corresponding Lar^13.2/Lar^5.5 transheterozygote. (Fox and Zinn, 2005) Sdc¹⁰⁶⁰⁸ mutants display ISNb bypass at a similar rate to wild-type embryos. (Fox and Zinn, 2005) Expression of dlp^{Scer\UAS.T:Ivir\HA1}, under the control of Scer\GAL4^elav-C155, rescues the medulla patterning defects of Sdc¹⁰⁶⁰⁸/Df(2R)48 mutants (in which Sara function has been rescued by Sara^Ubi.PJ). However, this expression fails to rescue the ERG defects. (Rawson et al., 2005) Half of Df(2R)48/Sdc¹⁰⁶⁰⁸ larvae (in which Sara function has been rescued by Sara^Ubi.PJ) show photoreceptor projection and lamina-plexus defects. At the pupal level, these mutants show crossover of R7 axons between medullary cartridges and defective axon pathfinding to the medulla, with a low penetrance of R7/R8 terminal disruption. Electrophysiological analysis shows that as adults, these mutants have grossly abnormal ERGs, with defective photoreceptor polarization and complete absence of on- and off-transients. (Rawson et al., 2005) Df(2R)48/Sdc¹⁰⁶⁰⁸ mutants (in which Sara function has been rescued by Sara^Ubi.PJ) have no rough eye phenotype and show no defects in the specification of photoreceptors, glia or lamina neurons. (Rawson, 2005) Expression of Sdc^Scer\UAS.cSa, under the control of Scer\GAL4^elav-C155, rescues the medulla cartridge crossover and R7/R8 termini disruption pupal phenotypes of Df(2R)48/Sdc¹⁰⁶⁰⁸ mutants (in which Sara function has been rescued by Sara^Ubi.PJ). However, this expression fails to rescue photoreceptor projection defects to the larval lamina or ERG abnormalities in adults. (Rawson et al., 2005) Sdc¹⁰⁶⁰⁸ embryos show a high frequency of axons crossing the midline in the central nervous system. (Johnson et al., 2004)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference wandering third instar larval stage imaginal disc (Meister and Braun, 1995.10) embryonic/larval brain (Meister and Braun, 1995.10) embryonic/larval midgut \| restricted (Meister and Braun, 1995.10) ring gland (Meister and Braun, 1995.10) embryonic/larval fat body (Meister and Braun, 1995.10) embryonic/larval hindgut \| restricted (Meister and Braun, 1995.10) adult stage adult brain (Vosshall, 1998.8.10)
Additional Information
Statement Reference In adult, expression throughout brain. (Vosshall, 1998.8.10) In wandering third instar larva, high levels of expression in ring gland and fat body; moderate levels in imaginal discs (which discs not specified) and brain; weak expression in gut. (Meister and Braun, 1995.10)

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)10608 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	Location 2R:16984308-16984309 confirmed by FlyBase alignment of dbGSS accession AQ034166 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P2377 (Meister and Braun, 1995.10) P{PZ}l(2)10608¹⁰⁶⁰⁸ (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{PZ}Sdc¹⁰⁶⁰⁸ (FlyBase, 2005, FlyBase, 1992-, ) P{ry^+t7.2=PZ}l(2)10608¹⁰⁶⁰⁸ (FlyBase, 1992-) sdc¹⁰⁶⁰⁸ (Ren et al., 2009)
Secondary FlyBase IDs
	FBti0000441
References ( 18 )
Research paper	Ren et al., 2009, J. Neurosci. 29(26): 8539--8550 Cell type-specific requirements for heparan sulfate biosynthesis at the Drosophila neuromuscular junction: effects on synapse function, membrane trafficking, and mitochondrial localization. [FBrf0208233] Johnson et al., 2006, Neuron 49(4): 517--531 The HSPGs Syndecan and Dallylike bind the receptor phosphatase LAR and exert distinct effects on synaptic development. [FBrf0191320] Fox and Zinn, 2005, Curr. Biol. 15(19): 1701--1711 The heparan sulfate proteoglycan syndecan is an in vivo ligand for the Drosophila LAR receptor tyrosine phosphatase. [FBrf0190011] Rawson et al., 2005, Curr. Biol. 15(9): 833--838 The heparan sulfate proteoglycans daily-like and syndecan have distinct functions in axon guidance and visual-system assembly in Drosophila. [FBrf0187328] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Johnson et al., 2004, Curr. Biol. 14(6): 499--504 Axonal heparan sulfate proteoglycans regulate the distribution and efficiency of the repellent slit during midline axon guidance. [FBrf0174485] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Supplementary material	Fox and Zinn, 2005, Curr. Biol. 15(19): Supplemental data. [FBrf0191745] Rawson, 2005, Curr. Biol. 15(9): Document S1. Experimental Procedures and Four Figures. [FBrf0191734]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] Vosshall, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103142] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
Abstract	Lincecum et al., 2000, Mol. Biol. Cell 11(Suppl.): 145a--146a Conservation of function in syndecans: Drosophila syndecan and murine syndecan-1 cytoplasmic domains are required for epithelial morphogenesis. [FBrf0138476]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{PZ}Sdc10608 Insertion

Dmel\P{PZ}Sdc¹⁰⁶⁰⁸ Insertion