A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{PZ}RpLP001544 Insertion

General Information
Symbol Dmel\P{PZ}RpLP001544 Species D. melanogaster
Name FlyBase ID FBti0005432
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, RpLP0 Viability / fertility
Causes allele(s) Ecol\lacZRpLP0-01544, RpLP001544 Stock availability 1 publicly available
LINE ID l(3)01544
Genomic Location
Chromosomal location 3L ( 79B2 ) Sequence location 3L:22,069,233..22,069,233 [+]
Map ( GBrowse ) detailed view FBti0060231 FBti0060241 FBti0060232 FBti0020200 FBti0125858_2 FBti0125858_1 FBti0102317 FBti0056161 FBti0071002 FBti0103348 FBti0044761 FBti0033714 FBti0027375 FBti0052491 FBti0125859_2 FBti0125859_1 FBti0109711 FBti0056892 FBti0034820 FBti0112562 FBti0074794 FBti0029129 FBti0005432 FBti0045592 FBti0049237
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Description
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:22,069,233..22,069,233 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
79B2 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026171
(FlyBase, 1992-,  
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999, Meister and Braun, 1995.10, Vosshall, 1998.8.10)
RpLP0
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZRpLP0-01544
(BDGP Project Members, 1994-1999, Meister and Braun, 1995.10, Vosshall, 1998.8.10)
RpLP001544
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Frolov and Birchler, 1998)
Lethality
References
lethal | recessive
(Spradling et al., 1999, Frolov and Birchler, 1998)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage  
embryonic/larval brain  
(Meister and Braun, 1995.10)
gonad  
(Meister and Braun, 1995.10)
adult stage  
third segment of antenna  
(Vosshall, 1998.8.10)
optic lobe  
(Vosshall, 1998.8.10)
adult fat body  
(Vosshall, 1998.8.10)
Additional Information
Statement
Reference
In wandering third instar, weak β-gal staining in the larval brain and gonad.
(Meister and Braun, 1995.10)
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)01544
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11537
P{PZ}RpLP001544 ry506/TM3, ryRK Sb1 Ser1
hide Linkouts
hide Comments
Location 3L:22009974-22009975 confirmed by FlyBase alignment of dbGSS accession AQ026171 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P1537
(Meister and Braun, 1995.10)
P{PZ}l(3)0154401544
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{PZ}RpLP001544
(FlyBase, 2005, FlyBase, 1992-)
P{PZ}RpP001544
(FlyBase, 1992-)
P{ry+t7.2=PZ}l(3)0154401544
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0000495
hide References ( 10 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Frolov and Birchler, 1998, Genetics 150(4): 1487--1495
Mutation in P0, a dual function ribosomal Protein/Apurinic/Apyrimidinic endonuclease, modifies gene expression and position effect variegation in Drosophila. [FBrf0105827]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase
Vosshall, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103142]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]