A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{PZ}srp01549 Insertion

General Information
Symbol Dmel\P{PZ}srp01549 Species D. melanogaster
Name FlyBase ID FBti0005433
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, srp Viability / fertility
Causes allele(s) Ecol\lacZsrp-01549, srp01549 Stock availability 1 publicly available
LINE ID l(3)01549
Genomic Location
Chromosomal location 3R ( 89A11 ) Sequence location 3R:11,820,303..11,820,303 [+]
Map ( GBrowse ) detailed view FBti0062243 FBti0005046 FBti0049482 FBti0050752 FBti0053989 FBti0057060 FBti0056991 FBti0005433
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:11,820,303..11,820,303 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
89A11 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026172
(FlyBase, 1992-,  
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999, Lamka and Lipshitz, 1999, Artero et al., 2003, Vosshall, 1998.8.10)
srp
(Fattah, 1996.4.29)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZsrp-01549
(BDGP Project Members, 1994-1999, Lamka and Lipshitz, 1999, Artero et al., 2003, Vosshall, 1998.8.10)
srp01549
(Fattah, 1996.4.29, BDGP Project Members, 1994-1999, Lamka and Lipshitz, 1999, Rehorn et al., 1996, Spradling et al., 1999)
Lethality
References
lethal | embryonic stage | recessive
(Perrimon et al., 1996)
lethal | recessive
(Rehorn et al., 1996, Spradling et al., 1999)
Sterility
References
hide Phenotype Manifest In
abdomen | posterior
(Perrimon et al., 1996)
crystal cell
(Milchanowski et al., 2004)
embryonic/first instar larval cuticle
(Perrimon et al., 1996)
plasmatocyte
(Milchanowski et al., 2004)
hide Detailed Description
Statement
Reference
The average number of crystal cells per embryo is reduced (to 1) in homozygous stage 13-14 embryos compared to wild type (36 +/- 2.2 cells on average). The number of plasmatocytes is also significantly reduced compared to wild-type embryos.
(Milchanowski et al., 2004)
Germline clones produce eggs with patterning defects: tail region remains on dorsal side.
(Perrimon et al., 1996)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
In wandering third instar larva, no detectable β-gal staining.
(Meister and Braun, 1995.10)
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)01549
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11538
ry506 P{PZ}srp01549/TM3, ryRK Sb1 Ser1
hide Linkouts
hide Comments
Location 3R:11820303-11820304 confirmed by FlyBase alignment of dbGSS accession AQ026172 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P1538
(Meister and Braun, 1995.10)
P{PZ}l(3)0154901549
(FlyBase, 1992-)
P{PZ}srp01549
(FlyBase, 2005, Fattah, 1996.4.29, FlyBase, 1992-)
P{ry+t7.2=PZ}srp01549
(FlyBase, 1992-)
serpent-lacZ
(Artero et al., 2003)
Secondary FlyBase IDs
  • FBti0000738
hide References ( 15 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Milchanowski et al., 2004, Genetics 168(1): 325--339
Identification and characterization of genes involved in embryonic crystal cell formation during Drosophila hematopoiesis. [FBrf0180297]
Artero et al., 2003, Development 130(25): 6257--6272
Notch and Ras signaling pathway effector genes expressed in fusion competent and founder cells during Drosophila myogenesis. [FBrf0167513]
Lamka and Lipshitz, 1999, Dev. Biol. 214(1): 102--112
Role of the amnioserosa in germ band retraction of the Drosophila melanogaster embryo. [FBrf0111406]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Perrimon et al., 1996, Genetics 144(4): 1681--1692
Zygotic lethal mutations with maternal effect phenotypes in Drosophila melanogaster. [FBrf0091142]
Rehorn et al., 1996, Development 122(12): 4023--4031
A molecular aspect of hematopoiesis and endoderm development common to vertebrates and Drosophila. [FBrf0091301]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase
Vosshall, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103142]
Fattah, 1996.4.29, [title not yet available]
[title not yet available] [FBrf0086767]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]