Dmel\P{PZ}pum⁰¹⁶⁸⁸ Insertion

General Information
Symbol	Dmel\P{PZ}pum⁰¹⁶⁸⁸	Species	D. melanogaster
Name		FlyBase ID	FBti0005438
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, pum	Viability / fertility
Causes allele(s)	Ecol\lacZ^pum-01688, pum⁰¹⁶⁸⁸	Stock availability	1 publicly available
LINE ID	fs(3)01688 l(3)01688
Genomic Location
Chromosomal location	3R ( 85D1 )	Sequence location	3R:5,052,560..5,052,560 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{PZ}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue. Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	3R:5,052,560..5,052,560 [+]
Orientation
Cytological location (computed by FlyBase)	85D1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	85D2-85D3 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	G00616 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999, Vosshall, 1998.8.10, Russell et al., 1998) pum (Lin and Spradling, 1997)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^pum-01688 (BDGP Project Members, 1994-1999, Vosshall, 1998.8.10, Russell et al., 1998) pum⁰¹⁶⁸⁸ (Lin and Spradling, 1997, Spradling, 1999.12.9, BDGP Project Members, 1994-1999, Parisi and Lin, 1999, Spradling et al., 1999, Schweers et al., 2002)

Lethality References lethal \| recessive (Spradling et al., 1999) partially lethal - majority die
Sterility References female sterile (Lin and Spradling, 1997)
Phenotype Manifest In
	female germline stem cell \| larval stage (Parisi and Lin, 1999) macrochaeta \| ectopic (Kim et al., 2012) metathoracic tergum (Kim et al., 2012) motor neuron (Kraut et al., 2001) nurse cell (Parisi and Lin, 1999) oocyte (Parisi and Lin, 1999) ovariole \| germline clone (Parisi and Lin, 1999) ovary (Parisi and Lin, 1999, Lin and Spradling, 1997) wing vein \| ectopic (Kim et al., 2012)
Detailed Description
Statement Reference Homozygous pum[01688] escapers have rare extra wing veins. Trans-heterozygous pum[7]/pum[01688] flies also display extra wing veins. Trans-heterozygotes of pum[1]/pum[01688] display extra wing veins. Trans-heterozygotes of pum[3]/pum[01688] display extra wing veins and extra macrochaetae on their notum. The penetrance of the notum phenotype is incomplete. Trans-heterozygotes of pum[Msc]/pum[01688] display extra wing veins and extra macrochaetae on their notum. The penetrance of the notum phenotype is incomplete. The notum of pum[7]/pum[01688] escapers display extra macrochaetae. The penetrance of the notum phenotype is incomplete. The notum of homozygous pum[01688] escapers display extra macrochaetae. The penetrance of the notum phenotype is incomplete. (Kim et al., 2012) Heterozygous pum[01688] greatly enhances the ectopic macrochaeta phenotype induced by Egfr[Scer\UAS.cUa] overexpression in the notum under the control of Scer\GAL4[sca-537.4]. (Kim et al., 2012) Complementation tested against pum^bem female sterility phenotype and gravitaxis and flight defects. (Schweers et al., 2002) Mutants have a motor axon pathfinding phenotype. (Kraut et al., 2001) Homozygous females do not lay eggs. Most homozygous larvae survive the pupal stage to eclosion. 40% of homozygous ovaries from third instar larvae have a normal number of germline cells and 60% have an overproliferated germline. By the adult stage, 9.5% of homozygous females have germlineless ovaries, 62% have "differentiated ovaries" (containing recognisable egg chambers and/or germline cells in at least one ovariole) and 28.5% of females have one germlineless and one differentiated ovary. Developing mutant egg chambers sometimes contain few nurse cells but no oocytes. Homozygous female germline clones induced at the third larval instar stage produce "differentiated" ovarioles that contain on average 2.8 +/- 1.7 mature egg chambers. Ovarioles containing a single mature egg and a rudimentary germarium are also seen. Females containing homozygous female germline clones achieve an oviposition efficiency of 85% (for absolute number of eggs laid by the females). These eggs are (derived from females containing homozygous germline clones mated to homozygous males) sometimes capable of developing into adulthood. (Parisi and Lin, 1999) Partially complements the embryonic lethality of the maternal effect alleles pum¹, pum^Msc and pum⁷, resulting in viable yet sterile progeny. Partially complements pum⁰²⁰⁰³ and pum⁰³²⁰³ for oogenic defects and embryonic lethality. (Parisi and Lin, 1999) Small ovary phenotype. (Lin and Spradling, 1997)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference wandering third instar larval stage imaginal disc (Meister and Braun, 1995.10) adult stage third segment of antenna (Vosshall, 1998.8.10)
Additional Information
Statement Reference In wandering third instar larva, expression in imaginal discs (which discs not specified). (Meister and Braun, 1995.10)

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	fs(3)01688 (Gene Disruption Project members, 2001-) l(3)01688 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	11544 P{PZ}pum⁰¹⁶⁸⁸ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹
Linkouts
Comments
	This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu). (FlyBase, 1992-)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	fs(3)01688 (FlyBase, 1992-) P{PZ}l(3)01688⁰¹⁶⁸⁸ (FlyBase, 1992-) P{PZ}pum⁰¹⁶⁸⁸ (Lin and Spradling, 1997, FlyBase, 1992-, ) P{ry^+t7.2=PZ}l(3)01688⁰¹⁶⁸⁸ (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000501
References ( 16 )
Research paper	Kim et al., 2012, PLoS ONE 7(4): e34016 Negative Regulation of EGFR/MAPK Pathway by Pumilio in Drosophila melanogaster. [FBrf0218126] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Schweers et al., 2002, Genetics 161(3): 1177--1185 The Drosophila melanogaster translational repressor pumilio regulates neuronal excitability. [FBrf0151333] Parisi and Lin, 1999, Genetics 153(1): 235--250 The Drosophila pumilio gene encodes two functional protein isoforms that play multiple roles in germline development, gonadogenesis, oogenesis and embryogenesis. [FBrf0111454] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Russell et al., 1998, Genome 41(1): 7--13 Lethal P-lacZ insertion lines expressed during pattern respecification in the imaginal discs of Drosophila. [FBrf0102000] Lin and Spradling, 1997, Development 124(12): 2463--2476 A novel group of pumilio mutations affects the asymmetric division of germline stem cells in the Drosophila ovary. [FBrf0095585] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Supplementary material	Kraut et al., 2001, Curr. Biol. 11(6): A gain-of-function screen for genes controlling motor axon guidance and synaptogenesis in Drosophila. [FBrf0135968]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] Spradling, 1999.12.9, Helping FlyBase with P-element paper. Helping FlyBase with P-element paper. [FBrf0125024] Vosshall, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103142] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{PZ}pum01688 Insertion

Dmel\P{PZ}pum⁰¹⁶⁸⁸ Insertion