Dmel\P{PZ}Hph⁰²²⁵⁵ Insertion

General Information
Symbol	Dmel\P{PZ}Hph⁰²²⁵⁵	Species	D. melanogaster
Name		FlyBase ID	FBti0005451
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, Hph	Viability / fertility
Causes allele(s)	Ecol\lacZ^Hph-02255, Hph⁰²²⁵⁵	Stock availability	1 publicly available
LINE ID	l(3)02255
Genomic Location
Chromosomal location	3R ( 82F8 )	Sequence location	3R:1,090,925..1,090,925 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{PZ}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue. Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3R:1,090,925..1,090,925 [-] (FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)	82F8 ( inferred by FlyBase from sequence location )
Cytological location (reported)	82F4-82F7 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ026177 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999, French et al., 2003, Vosshall, 1998.8.10) Hph (Centanin et al., 2005, BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^Hph-02255 (BDGP Project Members, 1994-1999, French et al., 2003, Vosshall, 1998.8.10) Hph⁰²²⁵⁵ (BDGP Project Members, 1994-1999, Lavista-Llanos et al., 2002, Frei and Edgar, 2004, Centanin et al., 2005, Spradling et al., 1999)

Lethality References lethal (Lavista-Llanos et al., 2002) lethal \| first instar larval stage (Centanin et al., 2005) lethal \| larval stage (Frei and Edgar, 2004) lethal \| recessive (Lavista-Llanos et al., 2002, BDGP Project Members, 1994-1999, Spradling et al., 1999) partially lethal - majority die \| embryonic stage (Frei and Edgar, 2004) partially lethal - majority die \| larval stage (Frei and Edgar, 2004) partially lethal - majority die \| pupal stage (Frei and Edgar, 2004)
Sterility References
Phenotype Manifest In
	embryonic/larval fat body \| somatic clone (Frei and Edgar, 2004)
Detailed Description
Statement Reference Clones of cells in the wing disc co-expressing Mmus\Ccnd1[Scer\UAS.cDa] and Hsap\CDK4[Scer\UAS.cDa] under the control of Scer\GAL4[Act5C.PP] show a 60-70% increase in median clone size compared to wild-type controls. Hph[02255]/+ suppresses this phenotype. (Datar et al., 2006) Hph⁰²²⁵⁵/+ partially suppresses the increase in size of ommatidia and interommatidial bristles seen in seen in the eyes of Cdk4^Scer\UAS.cMa; CycD^Scer\UAS.cDa; Scer\GAL4^GMR.PF flies. FACS analysis of dissociated eye discs from Cdk4^Scer\UAS.cMa; CycD^Scer\UAS.cDa; Scer\GAL4^GMR.PF indicates that Scer\GAL4 expressing cells are larger than non-expressing and a decreased proportion are in S-phase. The increase in cell size, but not the reduction in S-phase, is partially suppressed by Hph⁰²²⁵⁵/+. FACS analysis of dissociated eye discs from Cdk4^Scer\UAS.cMa; CycD^Scer\UAS.cDa; Scer\GAL4^GMR.PF indicates that Scer\GAL4 expressing cells are larger than non-expressing. This increase in cell size is partially suppressed by Hph⁰²²⁵⁵/+. (Frei and Edgar, 2004) Most Hph⁰²²⁵⁵/Hph⁰²²⁵⁵ or Hph⁰²²⁵⁵/Df(3R)3-4 animals maily die during embryogenesis, but a few hatch into larva with severe growth defects that die die within 2 to 3 days. Hph^S030304/Hph⁰²²⁵⁵ animals develop normally until pupariation, but very few escaper adults eclose. These escapers are smaller than their heterozygous siblings (18% lighter), but have normal body proportions. Cells in Hph⁰²²⁵⁵ homozygous somatic clones (induced during embryogenesis) in the fat body of late third instar larvae are smaller and contain less DNA than heterozygous neighboring cells. (Frei and Edgar, 2004) P{UAS-Hph.F}II.1; P{UAS-Hph.F}II.2; Scer\GAL4^hs.PB rescues the lethality of Hph⁰²²⁵⁵/Hph^S030304 even in the absence of heat shock. Hph^Scer\UAS.cFa rescues the increase in ommatidial size in Cdk4^Scer\UAS.cMa; CycD^Scer\UAS.cDa; Scer\GAL4^GMR.PF suppressed by Hph⁰²²⁵⁵/+. (Frei and Edgar, 2004)
Expression Data
Reporter Expression

Additional Information
Statement Reference In wandering third instar larva, no detectable β-gal staining. (Meister and Braun, 1995.10)

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)02255 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	11561 P{PZ}Hph⁰²²⁵⁵ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹
Linkouts
Comments
	Location 3R:1090924-1090925 determined by FlyBase alignment of dbGSS accession AQ026177 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P1561 (Meister and Braun, 1995.10) P{PZ}Hph⁰²²⁵⁵ (FlyBase, 2005, FlyBase, 1992-, ) P{PZ}l(3)02255 (Ostrowski et al., 2002) P{PZ}l(3)02255⁰²²⁵⁵ (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{ry^+t7.2=PZ}l(3)02255⁰²²⁵⁵ (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000512
References ( 16 )
Research paper	Datar et al., 2006, Cell Cycle 5(6): 647--652 Mammalian cyclin D1/Cdk4 complexes induce cell growth in Drosophila. [FBrf0189936] Centanin et al., 2005, EMBO Rep. 6(11): 1070--1075 Reversion of lethality and growth defects in Fatiga oxygen-sensor mutant flies by loss of Hypoxia-Inducible Factor-alpha/Sima. [FBrf0190371] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Frei and Edgar, 2004, Dev. Cell 6(2): 241--251 Drosophila cyclin D/Cdk4 requires Hif-1 prolyl hydroxylase to drive cell growth. [FBrf0174538] French et al., 2003, Dev. Biol. 253(1): 18--35 The Drosophila female sterile mutation twin peaks is a novel allele of tramtrack and reveals a requirement for Ttk69 in epithelial morphogenesis. [FBrf0155483] Lavista-Llanos et al., 2002, Mol. Cell. Biol. 22(19): 6842--6853 Control of the hypoxic response in Drosophila melanogaster by the basic helix-loop-helix PAS protein Similar. [FBrf0152214] Ostrowski et al., 2002, Genetics 161(1): 171--182 Genetic control of cuticle formation during embryonic development of Drosophila melanogaster. [FBrf0149011] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] Vosshall, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103142] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{PZ}Hph02255 Insertion

Dmel\P{PZ}Hph⁰²²⁵⁵ Insertion