Dmel\P{PZ}Baldspot⁰²²⁸¹ Insertion

General Information
Symbol	Dmel\P{PZ}Baldspot⁰²²⁸¹	Species	D. melanogaster
Name		FlyBase ID	FBti0005453
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Baldspot, Ecol\lacZ	Viability / fertility
Causes allele(s)	Baldspot⁰²²⁸¹, Ecol\lacZ^{Baldspot-02281}	Stock availability	1 publicly available
LINE ID	l(3)02281
Genomic Location
Chromosomal location	3L ( 73B5 )	Sequence location	3L:16,654,211..16,654,211
Map ( GBrowse )
Recent Updates
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FB2011_10
FB2012_01	Unknown in situ Controlled Vocabulary Terms larval stage testis terminal epithelium of testis embryonic/larval central nervous system spermatogenesis apical head cyst cell germarium region 1 spermatogenesis germarium region 3 tail cyst cell female male adult stage
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:16,654,211..16,654,211 (Jung et al., 2007) 3L:16,654,382..16,654,382 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	73B5 ( inferred by FlyBase from sequence location )
Cytological location (reported)	73B5-73B6 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location	The insertion site is based on the reported location at 360bp into the first Baldspot intron. The orientation of the insertion was not reported. (Jung et al., 2007)
Sequence Data
Flanking sequence	AQ026179 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Baldspot (BDGP Project Members, 1994-1999, Jung et al., 2007) Ecol\lacZ (BDGP Project Members, 1994-1999, Russell et al., 1998, Vosshall, 1998.8.10)
Alleles and Phenotypes
Causes alleles	Baldspot⁰²²⁸¹ (BDGP Project Members, 1994-1999, Spradling et al., 1999, Misra, 2000.11.18, Jung et al., 2007) Ecol\lacZ^{Baldspot-02281} (BDGP Project Members, 1994-1999, Russell et al., 1998, Vosshall, 1998.8.10)

Lethality References lethal \| recessive (Spradling et al., 1999, Jung et al., 2007) semi-lethal \| recessive (Berkeley Drosophila Genome Project, 1993.6.1, )
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference Body colour: dark. (Berkeley Drosophila Genome Project, 1993.6.1)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference larval stage \| male testis \| apical (Jung et al., 2007) Comment:expression observed in a small number of cells in the apical part of the testis embryonic/larval fat body (Jung et al., 2007) wandering third instar larval stage imaginal disc (Meister and Braun, 1995.10) embryonic/larval fat body (Meister and Braun, 1995.10) embryonic/larval midgut (Meister and Braun, 1995.10) embryonic/larval central nervous system (Jung et al., 2007) adult stage \| male terminal epithelium of testis (Jung et al., 2007) adult stage \| male & spermatogenesis head cyst cell (Jung et al., 2007) Comment:postmeiotic cyst cells tail cyst cell (Jung et al., 2007) Comment:postmeiotic cyst cells in situ Stage Tissue/Position (including subcellular localization) Reference adult stage \| female germarium region 1 -- germarium region 3 (Jung et al., 2007)
Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)02281 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	11563 P{PZ}Baldspot⁰²²⁸¹ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹
Linkouts
Comments
	Location 3L:16626541-16626542 confirmed by FlyBase alignment of dbGSS accession AQ026179 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{PZ}Baldspot⁰²²⁸¹ (FlyBase, 2005, FlyBase, 1992-, ) P{PZ}l(3)02281⁰²²⁸¹ (FlyBase, 1992-) P{PZ}l(3)neo22⁰²²⁸¹ (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{ry^+t7.2=PZ}l(3)neo22⁰²²⁸¹ (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000668
References ( 13 )
Research paper	Jung et al., 2007, J. Cell Sci. 120(16): 2924--2934 The fatty acid elongase NOA is necessary for viability and has a somatic role in Drosophila sperm development. [FBrf0200976] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Russell et al., 1998, Genome 41(1): 7--13 Lethal P-lacZ insertion lines expressed during pattern respecification in the imaginal discs of Drosophila. [FBrf0102000] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase	Misra, 2000.11.18, nc5 report, third installment. nc5 report, third installment. [FBrf0132131] Vosshall, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103142] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] Berkeley Drosophila Genome Project, 1993.6.1, Insertion alleles. Insertion alleles. [FBrf0122635]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{PZ}Baldspot02281 Insertion

Dmel\P{PZ}Baldspot⁰²²⁸¹ Insertion