A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{PZ}lqfR03685 Insertion

General Information
Symbol Dmel\P{PZ}lqfR03685 Species D. melanogaster
Name FlyBase ID FBti0005483
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, lqfR Viability / fertility
Causes allele(s) Ecol\lacZlqfR-03685, lqfR03685 Stock availability 1 publicly available
LINE ID l(3)03685
Genomic Location
Chromosomal location 3R ( 94A12 ) Sequence location 3R:18,237,073..18,237,073 [-]
Map ( GBrowse ) detailed view FBti0048817 FBti0045900 FBti0066251 FBti0049475 FBti0130152 FBti0005483 FBti0056177 FBti0126454 FBti0128245 FBti0126211_1 FBti0126211_2 FBti0126212 FBti0128353
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Description
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This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:18,237,073..18,237,073 [-]
(FlyBase, 2005)
Orientation
?
(Lloyd et al., 2000)
Cytological location
(computed by FlyBase)
94A12 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
94A8--94A12 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026188
(FlyBase, 1992-,  
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999)
lqfR
(BDGP Project Members, 1994-1999, Lee et al., 2009)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZlqfR-03685
(BDGP Project Members, 1994-1999)
lqfR03685
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Lee et al., 2009)
Lethality
References
lethal | pupal stage
(Lee et al., 2009)
lethal | recessive
(Spradling et al., 1999)
semi-lethal
 
semi-lethal | pharate adult stage | recessive
(Lee et al., 2009)
semi-lethal | recessive
(Berkeley Drosophila Genome Project, 1993.6.1)
Sterility
References
hide Phenotype Manifest In
anterior scutellar bristle | ectopic
(Lee et al., 2009)
eye
(Lee et al., 2009)
eye disc
(Lee et al., 2009)
ommatidium
(Lee et al., 2009)
wing
(Lee et al., 2009)
wing hair
(Lee et al., 2009)
hide Detailed Description
Statement
Reference
At 18[o]C, homozygous eclose and appear morphologically normal. Homozygotes show semi-lethality at 25[o], with most homozygotes dying fully formed in their pupal cases. Adult escapers often have obvious morphological defects; the eyes are kidney-shaped and rough, the wings are notched and the wing hair patterns may suggest planar cell polarity defects. Duplications of the anterior scutellar bristles are also seen. The ommatidia near the morphogenetic furrow are abnormally mature in mutant larval eye discs, and the rows of developing ommatidia are V-shaped, rather than straight as in wild-type discs. In adult flies, the eyes are rough along the equator, and the ommatidia in this region have a wide variety of defects, including ommatidia with too many or too few photoreceptors, irregular polarity and ommatidial fusions. Away from the equator, the ommatidia are normal.
(Lee et al., 2009)
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage  
embryonic/larval salivary gland  
(Meister and Braun, 1995.10)
embryonic/larval midgut  
(Meister and Braun, 1995.10)
embryonic/larval proventriculus  
(Meister and Braun, 1995.10)
Additional Information
Statement
Reference
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)03685
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11601
ry506 P{PZ}lqfR03685/TM3, ryRK Sb1 Ser1
hide Linkouts
hide Comments
Location 3R:18237072-18237073 determined by FlyBase alignment of dbGSS accession AQ026188 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P1601
(Meister and Braun, 1995.10)
P{PZ}epsin-like03685
(FlyBase, 1992-)
P{PZ}l(3)0368503685
(FlyBase, 1992-, FlyBase, 2005, BDGP Project Members, 1994-1999)
P{PZ}lqfR03685
 
P{ry+t7.2=PZ}l(3)0368503685
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0000540
hide References ( 11 )
Research paper
Lee et al., 2009, Dev. Biol. 331(1): 1--13
Drosophila liquid facets-Related encodes Golgi epsin and is an essential gene required for cell proliferation, growth, and patterning. [FBrf0208139]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Lloyd et al., 2000, Neuron 26(1): 45--50
A genome-wide search for synaptic vesicle cycle proteins in Drosophila. [FBrf0127207]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
Berkeley Drosophila Genome Project, 1993.6.1, Insertion alleles.
Insertion alleles. [FBrf0122635]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]