Dmel\P{PZ}mub⁰⁴⁰⁹³ Insertion

General Information
Symbol	Dmel\P{PZ}mub⁰⁴⁰⁹³	Species	D. melanogaster
Name		FlyBase ID	FBti0005504
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data
Affected gene(s)	Ecol\lacZ, mub	Viability / fertility
Causes allele(s)	Ecol\lacZ^mub-04093, mub⁰⁴⁰⁹³	Stock availability	1 publicly available
LINE ID	l(3)04093
Genomic Location
Chromosomal location	3L ( 79A2 )	Sequence location	3L:21,873,158..21,873,158 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:21,873,158..21,873,158 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	79A2 ( inferred by FlyBase from sequence location )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence	AQ034062 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999, Vosshall, 1998.8.10) mub (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^mub-04093 (BDGP Project Members, 1994-1999, Vosshall, 1998.8.10) mub⁰⁴⁰⁹³ (BDGP Project Members, 1994-1999, Spradling et al., 1999, Fernandez-Funez et al., 2000)

Lethality References lethal \| recessive (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference mub⁰⁴⁰⁹³ flies, in trans to Df(3L)Exel6137 flies very rarely eclose. Those that do exhibit defective locomotive behaviour (they are flightless and stick to the growth medium) and die within several days. The few eggs that are laid from these flies are phenotypically normal. Flies with mub⁰⁴⁰⁹³ in trans to Df(3L)ED4978 (which leaves the mub coding region intact but deletes part of the first mub intron, all of the first mub exon, and all 5\' flanking sequences) can eclose, although at a low frequency. They exhibit locomotive defects and live for up to 7 days. The few eggs that are laid from these flies are phenotypically normal. (Geng and MacDonald, 2007) A heterozygous mub⁰⁴⁰⁹³ background moderately enhances the dorsalisation phenotype seen when Imp^Scer\UAS.cGa is expressed under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (on the second). (Geng and MacDonald, 2007)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)04093 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	11624 P{PZ}mub⁰⁴⁰⁹³ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹
Linkouts
Comments
	Location 3L:21813899-21813900 confirmed by FlyBase alignment of dbGSS accession AQ034062 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{PZ}l(3)04093⁰⁴⁰⁹³ (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{PZ}mub⁰⁴⁰⁹³ (FlyBase, 2005, FlyBase, 1992-) P{ry^+t7.2=PZ}l(3)04093⁰⁴⁰⁹³ (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000559
References ( 10 )
Research paper	Geng and MacDonald, 2007, Fly 1(5): 259--267 Identification of genes that influence gurken expression. [FBrf0205043] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Fernandez-Funez et al., 2000, Nature 408(6808): 101--106 Identification of genes that modify ataxin-1-induced neurodegeneration. [FBrf0131287] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase	Vosshall, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103142]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{PZ}mub04093 Insertion

Dmel\P{PZ}mub⁰⁴⁰⁹³ Insertion