A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{PZ}Hmgcr04684 Insertion

General Information
Symbol Dmel\P{PZ}Hmgcr04684 Species D. melanogaster
Name FlyBase ID FBti0005515
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, Hmgcr Viability / fertility
Causes allele(s) Ecol\lacZ04684, Hmgcr04684 Stock availability 1 publicly available
LINE ID l(3)04684
Genomic Location
Chromosomal location 3R ( 95B1 ) Sequence location 3R:19,583,339..19,583,339 [+]
Map ( GBrowse ) detailed view FBti0056063 FBti0108351 FBti0099960 FBti0100949 FBti0024733 FBti0040121 FBti0005505 FBti0077962 FBti0065083 FBti0005515 FBti0028332 FBti0143549 FBti0044075 FBti0044102 FBti0103996 FBti0025151 FBti0126234_1 FBti0126234_2 FBti0042183 FBti0045487 FBti0130096 FBti0115909 FBti0059002 FBti0102696 FBti0102695 FBti0107252 FBti0025017 FBti0074890
hide Recent Updates
Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
Update Feed
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
atom feed
FB2012_01
Genes
Hmgcr
References
Belgacem and Martin, 2007, PLoS ONE 2:
FB2011_10
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:19,583,339..19,583,339 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
95B1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
95B1-95B3 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026202
(FlyBase, 1992-,  
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999, Vosshall, 1998.8.10)
Hmgcr
(BDGP Project Members, 1994-1999, Belgacem and Martin, 2007)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZ04684
(BDGP Project Members, 1994-1999, Vosshall, 1998.8.10)
Hmgcr04684
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Belgacem and Martin, 2007)
Lethality
References
lethal
(Belgacem and Martin, 2007)
lethal | recessive
(Spradling et al., 1999)
semi-lethal
 
semi-lethal | recessive
(Berkeley Drosophila Genome Project, 1993.6.1)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
Expression of Hmgcr[Scer\UAS.cvDa] under the control of Scer\GAL4[elav-C155] or Scer\GAL4[to.PD] fails to rescue the lethality found in Hmgcr[04684] homozygotes. Expression of Hmgcr[Scer\UAS.cvDa] under the control of Scer\GAL4[Scer\FRT.Act5C] completely rescues lethality, but does not rescue the sexual dimorphism. Expression of Hmgcr[Scer\UAS.cvDa] under the control of Scer\GAL4[da.G32] is sufficient to rescue both the lethality (partly in males - 75%, and completely in females) and the start/stop number (indicating locomotor activity). Targeted expression of Hmgcr[Scer\UAS.cvDa] under the control of Scer\GAL4[Scer\FRT.Act5C] and Scer\GAL4[Hmgcr.B] is sufficient to rescue the sexual dimorphism.
(Belgacem and Martin, 2007)
Hmgcr[04684] homozygotes are lethal when raised at 24[o]C. However, when reared at 19[o]C, 5 and 10% of males and females, respectively, which are developmentally delayed, attempt to survive until adulthood. Quantification of the locomotor activity of these flies indicates that the start/stop number for both sexes is the same. Hmgcr[04684] heterozygotes exhibit a wild-type phenotype.
(Belgacem and Martin, 2007)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
In wandering third instar larva, no detectable β-gal staining.
(Meister and Braun, 1995.10)
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)04684
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11635
ry506 P{PZ}Hmgcr04684/TM3, ryRK Sb1 Ser1
hide Linkouts
hide Comments
Location 3R:19583339-19583340 confirmed by FlyBase alignment of dbGSS accession AQ026202 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
hmgcr11635
(Belgacem and Martin, 2007)
P1635
(Meister and Braun, 1995.10)
P{PZ}Hmgcr04684
 
P{PZ}l(3)0468404684
(FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{ry+t7.2=PZ}l(3)0468404684
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0000569
hide References ( 14 )
Research paper
Belgacem and Martin, 2007, PLoS ONE 2:
Hmgcr in the Corpus Allatum Controls Sexual Dimorphism of Locomotor Activity and Body Size via the Insulin Pathway in Drosophila. [FBrf0192310]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Keller et al., 1998, Dev. Biol. 202(2): 157--171
A role for nautilus in the differentiation of muscle precursors. [FBrf0105257]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Supplementary material
Parks et al., 2004, Nature Genetics 36(3):
Supplementary Table 1: Deletions generated at Bloomington between trans-heterozygous P element insertions. [FBrf0179070]
Personal communication to FlyBase
Andrade and Cook, 2004.1.19, Isolation and Characterization of Df(3R)BSC48.
Isolation and Characterization of Df(3R)BSC48. [FBrf0173261]
Vosshall, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103142]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
Berkeley Drosophila Genome Project, 1993.6.1, Insertion alleles.
Insertion alleles. [FBrf0122635]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]