Dmel\P{PZ}Vha13⁰⁵¹¹³ Insertion

General Information
Symbol	Dmel\P{PZ}Vha13⁰⁵¹¹³	Species	D. melanogaster
Name		FlyBase ID	FBti0005527
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, Vha13	Viability / fertility
Causes allele(s)	Ecol\lacZ^Vha13-05113, Vha13⁰⁵¹¹³	Stock availability	1 publicly available
LINE ID	l(3)05113
Genomic Location
Chromosomal location	3R ( 92A11 )	Sequence location	3R:15,469,740..15,470,048 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{PZ}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue. Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3R:15,469,740..15,470,048 [-] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	92A11 ( inferred by FlyBase from sequence location )
Cytological location (reported)	92A13-92A14 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ073324 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999, Vosshall, 1998.8.10) Vha13 (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^Vha13-05113 (BDGP Project Members, 1994-1999, Vosshall, 1998.8.10) Vha13⁰⁵¹¹³ (BDGP Project Members, 1994-1999, Spradling et al., 1999)

Lethality References lethal \| embryonic stage \| rescuable maternal effect (Perrimon et al., 1996) lethal \| recessive (Perrimon et al., 1996, Spradling et al., 1999)
Sterility References
Phenotype Manifest In
	embryonic/first instar larval cuticle (Perrimon et al., 1996) head (Perrimon et al., 1996)
Detailed Description
Statement Reference Germline clones produce eggs with patterning defects: head defects. Lethality occurs during the embryonic and larval stages. (Perrimon et al., 1996)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference wandering third instar larval stage imaginal disc (Meister and Braun, 1995.10) adult stage lamina (Vosshall, 1998.8.10)
Additional Information
Statement Reference In wandering third instar larva, very low levels of β-gal signal in imaginal discs (which discs not specified). (Meister and Braun, 1995.10)

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)05113 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	11647 ry⁵⁰⁶ P{PZ}Vha13⁰⁵¹¹³/CxD, ry^BW
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P1647 (Meister and Braun, 1995.10) P{PZ}l(3)05113⁰⁵¹¹³ (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{PZ}Vha13⁰⁵¹¹³ (FlyBase, 1992-, ) P{ry^+t7.2=PZ}l(3)05113⁰⁵¹¹³ (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000581
References ( 10 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Perrimon et al., 1996, Genetics 144(4): 1681--1692 Zygotic lethal mutations with maternal effect phenotypes in Drosophila melanogaster. [FBrf0091142] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] Vosshall, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103142] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{PZ}Vha1305113 Insertion

Dmel\P{PZ}Vha13⁰⁵¹¹³ Insertion