A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{PZ}twr05614 Insertion

General Information
Symbol Dmel\P{PZ}twr05614 Species D. melanogaster
Name FlyBase ID FBti0005538
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, twr Viability / fertility
Causes allele(s) Ecol\lacZtwr-05614, twr05614 Stock availability 1 publicly available
LINE ID l(3)05614
Genomic Location
Chromosomal location 3R ( 84A1 ) Sequence location 3R:2,483,680..2,483,680
Map ( GBrowse )
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Description
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:2,483,680..2,483,680
(Wang et al., 2010)
3R:2,483,689..2,483,689 [+]
Orientation
Cytological location
(computed by FlyBase)
84A1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
84A4-84A5 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
G01166
(FlyBase, 1992-,  
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999, Vosshall, 1998.8.10, Russell et al., 1998)
twr
(BDGP Project Members, 1994-1999, Wang et al., 2010)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZtwr-05614
(BDGP Project Members, 1994-1999, Vosshall, 1998.8.10, Russell et al., 1998)
twr05614
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Beaton, 2000.1.31, Wang et al., 2010)
Lethality
References
lethal | recessive
(Spradling et al., 1999, Beaton, 2000.1.31, BDGP Project Members, 1994-1999)
Sterility
References
hide Phenotype Manifest In
crystal cell
(Milchanowski et al., 2004)
hide Detailed Description
Statement
Reference
The average number of crystal cells per embryo is reduced (to 14.1) in homozygous stage 13-14 embryos compared to wild type (36 +/- 2.2 cells on average). Mutant stage 14 embryos show cell cycle defects in the head region.
(Milchanowski et al., 2004)
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage  
imaginal disc  
(Meister and Braun, 1995.10)
embryonic/larval salivary gland  
(Meister and Braun, 1995.10)
gonad  
(Meister and Braun, 1995.10)
embryonic/larval proventriculus  
(Meister and Braun, 1995.10)
Additional Information
Statement
Reference
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)05614
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11663
P{PZ}twr05614 ry506/TM3, ryRK Sb1 Ser1
hide Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{PZ}l(3)0561405614
(FlyBase, 1992-)
P{PZ}twr05614
(BDGP Project Members, 1994-1999, FlyBase, 1992-, )
P{ry+t7.2=PZ}twr05614
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0000748
hide References ( 12 )
Research paper
Wang et al., 2010, Genome Res. 20(7): 981--988
Rapid identification of heterozygous mutations in Drosophila melanogaster using genomic capture sequencing. [FBrf0211197]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Milchanowski et al., 2004, Genetics 168(1): 325--339
Identification and characterization of genes involved in embryonic crystal cell formation during Drosophila hematopoiesis. [FBrf0180297]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Russell et al., 1998, Genome 41(1): 7--13
Lethal P-lacZ insertion lines expressed during pattern respecification in the imaginal discs of Drosophila. [FBrf0102000]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase
Beaton, 2000.1.31, BDGP P element Update.
BDGP P element Update. [FBrf0126832]
Vosshall, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103142]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]