A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{PZ}Atx206490 Insertion

General Information
Symbol Dmel\P{PZ}Atx206490 Species D. melanogaster
Name FlyBase ID FBti0005559
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) Atx2, Ecol\lacZ Viability / fertility
Causes allele(s) Atx206490, Ecol\lacZAtx2-06490 Stock availability 1 publicly available
LINE ID l(3)06490
Genomic Location
Chromosomal location 3R ( 88F4 ) Sequence location 3R:11,234,606..11,234,606 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0131589 FBti0075496 FBti0104681 FBti0011343 FBti0113320 FBti0110939 FBti0104547 FBti0007959 FBti0005559 FBti0074734 FBti0126484 FBti0046383
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Spradling et al., 1999, Bellen et al., 2004)
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Description
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What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:11,234,606..11,234,606 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
88F4 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026218
(FlyBase, 1992-,  
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Atx2
(BDGP Project Members, 1994-1999, Sofola et al., 2007)
Ecol\lacZ
(BDGP Project Members, 1994-1999, Russell et al., 1998, Vosshall, 1998.8.10)
hide Alleles and Phenotypes
Causes alleles
Atx206490
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Satterfield et al., 2002, Sofola et al., 2007)
Ecol\lacZAtx2-06490
(BDGP Project Members, 1994-1999, Russell et al., 1998, Vosshall, 1998.8.10)
Lethality
References
lethal | recessive
(Satterfield et al., 2002, BDGP Project Members, 1994-1999, Spradling et al., 1999)
Sterility
References
female sterile | germline clone
(Satterfield et al., 2002)
hide Phenotype Manifest In
eye | somatic clone
(Satterfield et al., 2002)
nurse cell & actin filament | germ-line clone
(Satterfield et al., 2002)
nurse cell & nucleus | germ-line clone
(Satterfield et al., 2002)
nurse cell | germline clone
(Satterfield et al., 2002)
ommatidium | somatic clone
(Satterfield et al., 2002)
oocyte | germline clone
(Satterfield et al., 2002)
hide Detailed Description
Statement
Reference
Homozygous clones in the retina result in a mildly disorganised ommatidial pattern and a slight reduction in eye size. Females carrying homozygous germ line clones are completely sterile and fail to lay eggs. Most egg chambers in these females can proceed normally through the early and middle stages of oogenesis, but arrest at the stage when the nurse cells rapidly transfer their contents to the oocyte. The arrested egg chambers have enlarged nurse cells and a small poorly developed oocyte. The actin filaments that normally tether the nurse cell nuclei mostly fail to form. The nurse cell nuclei are inappropriately positioned close to the ring canals and are sometimes seen to stretch partially through the ring canals.
(Satterfield et al., 2002)
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage  
adult brain | restricted  
(Vosshall, 1998.8.10)
adult central complex  
(Vosshall, 1998.8.10)
adult antennal lobe  
(Vosshall, 1998.8.10)
adult mushroom body  
(Vosshall, 1998.8.10)
Additional Information
Statement
Reference
In wandering third instar larva, no detectable β-gal staining.
(Meister and Braun, 1995.10)
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)06490
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11688
ry506 P{PZ}Atx206490/TM3, ryRK Sb1 Ser1
hide Linkouts
hide Comments
Location 3R:11234606-11234607 confirmed by FlyBase alignment of dbGSS accession AQ026218 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{PZ}Atx206490
(FlyBase, 2005, FlyBase, 1992-, )
P{PZ}l(3)0649006490
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{ry+t7.2=PZ}l(3)0649006490
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0000613
hide References ( 13 )
Research paper
Sofola et al., 2007, Neuron 55(4): 565--571
RNA-Binding proteins hnRNP A2/B1 and CUGBP1 suppress fragile X CGG premutation repeat-induced neurodegeneration in a Drosophila model of FXTAS. [FBrf0200897]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Satterfield et al., 2002, Genetics 162(4): 1687--1702
A Drosophila Homolog of the Polyglutamine Disease Gene SCA2 Is a Dosage-Sensitive Regulator of Actin Filament Formation. [FBrf0155519]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Russell et al., 1998, Genome 41(1): 7--13
Lethal P-lacZ insertion lines expressed during pattern respecification in the imaginal discs of Drosophila. [FBrf0102000]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
Vosshall, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103142]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]