FlyBase Insertion Report: Dmel\P{PZ}nsl1[06536]

General Information

Symbol

Dmel\P{PZ}nsl1⁰⁶⁵³⁶

Species

D. melanogaster

Name

FlyBase ID

FBti0005562

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(3)06536

Affected gene(s)

Ecol\lacZ, nsl1

Viability / fertility

Associated allele(s)

Ecol\lacZ⁰⁶⁵³⁶, nsl1⁰⁶⁵³⁶

Stock availability

None publicly available

Genomic Location

Chromosomal Location

3R ( 89A5 )

Sequence Location

3R:15,794,033..15,794,033 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3R:15,794,033..15,794,033 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

89A5 (inferred by FlyBase from sequence location)

Cytological location (reported)

89A4-89A5 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ034070

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

nsl1

(Lone et al., 2010, Yu et al., 2010, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ⁰⁶⁵³⁶

Phenotypic Class Phenotype Manifest in Detailed Description

nsl1⁰⁶⁵³⁶

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

larval brain

(Meister and Braun, 1995.10)

embryonic/larval fat body

(Meister and Braun, 1995.10)

adult stage

adult brain | restricted

(Vosshall, 1998.8.10)

adult fat body

(Vosshall, 1998.8.10)

adult central complex

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In wandering third instar larva, moderate levels of expression in brain and imaginal discs (which discs not specified); very low levels in fat body.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

nsl1

(Meister and Braun, 1995.10)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)06536

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Comments

Location 3R:11619755-11619756 confirmed by FlyBase alignment of dbGSS accession AQ034070 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

P (06536)

(Yu et al., 2010)

P1691

(Meister and Braun, 1995.10)

P{PZ}l(3)06536

(Lone et al., 2010)

P{PZ}l(3)06536⁰⁶⁵³⁶

(FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{PZ}nsl1⁰⁶⁵³⁶

P{PZ}wah⁰⁶⁵³⁶

P{ry^+t7.2=PZ}l(3)06536⁰⁶⁵³⁶

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000616

References (11)

Report Sections

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