Dmel\P{PZ}rept⁰⁶⁹⁴⁵ Insertion

General Information
Symbol	Dmel\P{PZ}rept⁰⁶⁹⁴⁵	Species	D. melanogaster
Name		FlyBase ID	FBti0005573
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, rept	Viability / fertility
Causes allele(s)	Ecol\lacZ^rept-06945, rept⁰⁶⁹⁴⁵	Stock availability	1 publicly available
LINE ID	l(3)06945
Genomic Location
Chromosomal location	3L ( 76A3 )	Sequence location	3L:19,248,597..19,248,597 [-]
Map ( GBrowse )
Recent Updates
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:19,248,597..19,248,597 [-] (FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)	76A3 ( inferred by FlyBase from sequence location )
Cytological location (reported)	76A3-76A4 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ026224 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999, Vosshall, 1998.8.10) rept (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^rept-06945 (BDGP Project Members, 1994-1999, Vosshall, 1998.8.10) rept⁰⁶⁹⁴⁵ (BDGP Project Members, 1994-1999, Spradling et al., 1999, Bauer et al., 2000, Qi et al., 2006)

Lethality References lethal \| recessive (Spradling et al., 1999)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference Heterozygous males do not have ectopic sex combs on the second and third legs. Females containing homozygous germline clones do not produce embryos. (Qi et al., 2006) rept⁰⁶⁹⁴⁵/+ enhances the Df(3L)Pc/+ phenotype; the frequency of second and third legs having an ectopic sex comb is increased (to 41% and 25% respectively) in the double heterozygous males. rept⁰⁶⁹⁴⁵ does not show an ectopic sex comb phenotype in either the second or third legs in double heterozygous combination with either esc¹ or Psc^k07834. 47% of rept⁰⁶⁹⁴⁵/+ ; Pcl¹¹/+ males have ectopic sex combs on the second leg and 4% have ectopic sex combs on the third leg. rept⁰⁶⁹⁴⁵ Pc¹¹ double heterozygous males have ectopic sex combs on the second and third legs (47% and 5% of flies respectively). rept⁰⁶⁹⁴⁵ Psc¹ double heterozygous males have ectopic sex combs on the second and third legs (40% and 2% of flies respectively). rept⁰⁶⁹⁴⁵ shows little or no interaction (in terms of ectopic sex comb phenotype) when in double heterozygous combination with one of Psc^vgD, esc²¹ or Pcl⁷. rept⁰⁶⁹⁴⁵/+ enhances the ph-p⁴¹⁰/Y ectopic sex comb phenotype; 100% of the double mutant flies have ectopic sex combs on both the second and third legs. (Qi et al., 2006)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference wandering third instar larval stage imaginal disc (Meister and Braun, 1995.10) embryonic/larval brain (Meister and Braun, 1995.10) gonad (Meister and Braun, 1995.10)
Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)06945 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	11706 P{PZ}rept⁰⁶⁹⁴⁵ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹
Linkouts
Comments
	Location 3L:19220755-19220756 determined by FlyBase alignment of dbGSS accession AQ026224 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{PZ}l(3)06945⁰⁶⁹⁴⁵ (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{PZ}rept⁰⁶⁹⁴⁵ (FlyBase, 2005, FlyBase, 1992-) P{ry^+t7.2=PZ}l(3)06945⁰⁶⁹⁴⁵ (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0000627
References ( 11 )
Research paper	Qi et al., 2006, Genetics 174(1): 241--251 Drosophila Reptin and other TIP60 complex components promote generation of silent chromatin. [FBrf0193464] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Bauer et al., 2000, EMBO J. 19(22): 6121--6130 Pontin52 and Reptin52 function as antagonistic regulators of -catenin signalling activity. [FBrf0132031] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase	Vosshall, 1998.8.10, Patterns of lacZ expression. Patterns of lacZ expression. [FBrf0103142] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{PZ}rept06945 Insertion

Dmel\P{PZ}rept⁰⁶⁹⁴⁵ Insertion